Gut microbiota modulate CD8+ T cell immunity in gastric cancer through Butyrate/GPR109A/HOPX
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Quality control
- Retracted paper
- Contamination issues suspected
- Batch effect issues suspected
- Uncontrolled confounding suspected
- Results are suspect (various reasons)
- Tags applied
Experiment 1
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Gastric cancer Ca body - stomach,ca greater curvature of stomach,Ca lesser curvature - stomach,cancer of stomach,gastric cancer,gastric cancer, intestinal,gastric neoplasm,malignant gastric neoplasm,malignant gastric tumor,malignant neoplasm of body of stomach,malignant neoplasm of lesser curve of stomach,malignant neoplasm of stomach,malignant neoplasm of the stomach,malignant stomach neoplasm,malignant tumor of body of stomach,malignant tumor of greater curve of stomach,malignant tumor of lesser curve of stomach,malignant tumor of stomach,malignant tumor of the stomach,stomach cancer,Gastric cancer
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Normal Individuals (N)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Gastric Cancer patients (GC)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Patients admitted to the hospital with Gastric cancer.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 20
- Group 1 sample size Number of subjects in the case (exposed) group
- 20
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- 3 months
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- Not specified
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- LEfSe
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 2
Alpha Diversity
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- decreased
Signature 1
Source: Figure 1A, Supplementary Figure 2A and Supplementary Figure 3A
Description: Differential analysis of microbial community composition between Gastric Cancer-patients (GC) and Normal Individuals (N).
Abundance in Group 1: increased abundance in Gastric Cancer patients (GC)
Revision editor(s): MyleeeA
Signature 2
Source: Figure 1A, Supplementary Figure 2A and Supplementary Figure 3A
Description: Differential analysis of microbial community composition between Gastric Cancer-patients (GC) and Normal Individuals (N).
Abundance in Group 1: decreased abundance in Gastric Cancer patients (GC)
Revision editor(s): MyleeeA
Experiment 2
Subjects
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Mus musculus
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Response to transplant Response to transplant,response to transplant
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Normal-Fecal Microbiota Transplant (F-N)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Gastric Cancer-Fecal Microbiota Transplant (F-CA)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Mice given Antibiotic (ABX) and transplanted with Gastric Cancer microbiota (F-CA) from human.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 6
- Group 1 sample size Number of subjects in the case (exposed) group
- 6
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- Not specified
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- decreased
Signature 1
Source: Figure 2H and Supplementary Figure 9A
Description: Differential analysis of microbial community composition between Gastric Cancer-Fecal Microbiota Transplant (F-CA) and Normal-Fecal Microbiota Transplant (F-N).
Abundance in Group 1: increased abundance in Gastric Cancer-Fecal Microbiota Transplant (F-CA)
Revision editor(s): MyleeeA, Anne-mariesharp
Signature 2
Source: Figure 2H and Supplementary Figure 9A
Description: Differential analysis of microbial community composition between Gastric Cancer-Fecal Microbiota Transplant (F-CA) and Normal-Fecal Microbiota Transplant (F-N).
Abundance in Group 1: decreased abundance in Gastric Cancer-Fecal Microbiota Transplant (F-CA)
Revision editor(s): MyleeeA, Anne-mariesharp
Experiment 3
Subjects
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Gastric juice Stomach secretion,Succus gastricus,Gastric juice,gastric juice
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Normal mouse (N)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Gastric Cancer Mouse (Ca)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Gastric cancer mouse- 20 g C57BL/6J mice at 6 weeks of age infected with Helicobacter pylori and given N-methyl-N-nitrosourea (MNU) plus abnormal feeding cycles, to induce gastric cancer.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 4
- Group 1 sample size Number of subjects in the case (exposed) group
- 5
Lab analysis
Statistical Analysis
Signature 1
Source: Supplementary Figure 4A
Description: Differential analysis of microbial community composition of gastric fluids between Gastric Cancer mouse and Normal mouse.
Abundance in Group 1: increased abundance in Gastric Cancer Mouse (Ca)
Revision editor(s): MyleeeA
Signature 2
Source: Supplementary Figure 4A
Description: Differential analysis of microbial community composition of gastric fluids between Gastric Cancer mouse and Normal mouse.
Abundance in Group 1: decreased abundance in Gastric Cancer Mouse (Ca)
NCBI | Quality Control | Links |
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Dubosiella | ||
Ligilactobacillus | ||
Methyloversatilis | ||
Pseudomonas | ||
Vibrio |
Revision editor(s): MyleeeA