Short-term pectin-enriched smoothie consumption has beneficial effects on the gut microbiota of low-fiber consumers/Experiment 2

From BugSigDB


Needs review

Curated date: 2025/04/28

Curator: Shulamite

Revision editor(s): Shulamite

Subjects

Location of subjects
Estonia
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Diet measurement Diet measurement,diet measurement
Group 0 name Corresponds to the control (unexposed) group for case-control studies
High-fiber intake group (HF)
Group 1 name Corresponds to the case (exposed) group for case-control studies
Low-fiber intake group (LF)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Participants who regularly consumed (<23g) less than 23g of dietary fiber per day.
Group 0 sample size Number of subjects in the control (unexposed) group
9
Group 1 sample size Number of subjects in the case (exposed) group
22
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
3 Months.

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
Mann-Whitney (Wilcoxon)
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05


Signature 1

Needs review

Curated date: 2025/04/28

Curator: Shulamite

Revision editor(s): Shulamite

Source: Fig 3B

Description: At lower levels of abundance, four genera show the difference between LF and HF intake groups in the base period.

Abundance in Group 1: decreased abundance in Low-fiber intake group (LF)

NCBI Quality ControlLinks
Coprococcus
Lactococcus
Akkermansia
Cloacibacillus

Revision editor(s): Shulamite

Signature 2

Needs review

Curated date: 2025/04/28

Curator: Shulamite

Revision editor(s): Shulamite

Source: Fig. 4A, Supplementary Table S3 and S4

Description: Altered genera in LF intake group. The pairwise comparison was performed using the Wilcoxon signed-rank test for statistical analysis to compare LPect and HPect effects on base period microbiota at the genus level (*P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, ns—not significant).

Abundance in Group 1: increased abundance in Low-fiber intake group (LF)

NCBI Quality ControlLinks
Lachnospira
Bacteroides

Revision editor(s): Shulamite