Short-term pectin-enriched smoothie consumption has beneficial effects on the gut microbiota of low-fiber consumers/Experiment 3

From BugSigDB


Needs review

Curated date: 2025/04/28

Curator: Shulamite

Revision editor(s): Shulamite, Anne-mariesharp

Subjects

Location of subjects
Estonia
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Diet measurement Diet measurement,diet measurement
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Base (LF intake group)
Group 1 name Corresponds to the case (exposed) group for case-control studies
HPect (LF intake group)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Participants consuming a low-fiber diet (<23 g/day, LF intake group), who received the high-pectin (HPect) smoothie during the intervention period.
Group 1 sample size Number of subjects in the case (exposed) group
22
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
3 months

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
Mann-Whitney (Wilcoxon)
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05


Signature 1

Needs review

Curated date: 2025/04/28

Curator: Shulamite

Revision editor(s): Shulamite, Anne-mariesharp

Source: Figure 4A, 6A, Table S3

Description: Altered genera in LF intake group (Base vs HPect). The pairwise comparison was performed using the Wilcoxon signed-rank test for statistical analysis to compare LPect and HPect effects on base period microbiota at the genus level

Abundance in Group 1: increased abundance in HPect (LF intake group)

NCBI Quality ControlLinks
Bacteroides
Lachnospira
Paraprevotella

Revision editor(s): Shulamite, Anne-mariesharp

Signature 2

Needs review

Curated date: 2025/05/01

Curator: Shulamite

Revision editor(s): Shulamite, Anne-mariesharp

Source: Figure 4A, 4C, 6A, Table S3

Description: Altered genera in LF intake group (Base vs HPect). The pairwise comparison was performed using the Wilcoxon signed-rank test for statistical analysis to compare LPect and HPect effects on base period microbiota at the genus level.

Abundance in Group 1: decreased abundance in HPect (LF intake group)

NCBI Quality ControlLinks
Coprococcus
Dorea
Enterococcus
Eubacterium ventriosum
Faecalicatena fissicatena
Holdemanella
Streptococcus
Subdoligranulum
[Ruminococcus] torques
Lactococcus
Evtepia

Revision editor(s): Shulamite, Anne-mariesharp