Short-term pectin-enriched smoothie consumption has beneficial effects on the gut microbiota of low-fiber consumers/Experiment 4
Subjects
- Location of subjects
- Estonia
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Diet measurement Diet measurement,diet measurement
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Low Fiber intake (LF baseline)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Low Fiber intake (LF LPect)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Participants who consumed smoothies with a low amount of dietary fiber. Especially pectin.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 14
- Group 1 sample size Number of subjects in the case (exposed) group
- 14
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- 3 Months.
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- Mann-Whitney (Wilcoxon)
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
Signature 1
Source: Fig. 4B, 4C, Supplementary Table S3 and S4
Description: Altered genera in LF intake group. The pairwise comparison was performed using the Wilcoxon signed-rank test for statistical analysis to compare LPect and HPect effects on base period microbiota at the genus level.
Abundance in Group 1: increased abundance in Low Fiber intake (LF LPect)
| NCBI | Quality Control | Links |
|---|---|---|
| Clostridium sp. CAG:352 | ||
| Colidextribacter | ||
| Oxalobacter |
Revision editor(s): Shulamite
Signature 2
Source: Fig. 4B, 4C, Supplementary Table S3 and S4
Description: Altered genera in LF intake group. The pairwise comparison was performed using the Wilcoxon signed-rank test for statistical analysis to compare LPect and HPect effects on base period microbiota at the genus level
Abundance in Group 1: decreased abundance in Low Fiber intake (LF LPect)
| NCBI | Quality Control | Links |
|---|---|---|
| Holdemania | ||
| Merdibacter | ||
| Peptococcus | ||
| Streptococcus | ||
| Turicibacter | ||
| Defluviitaleaceae_UCG-011Defluviitaleaceae_UCG-011 | ||
| UCG-009UCG-009 |
Revision editor(s): Shulamite
