Gastrointestinal Characteristics of Constipation from the Perspectives of Microbiome and Metabolome

Study information

Needs review

study design

laboratory experiment

Citation

PMID PubMed identifier for scientific articles.

38446304

DOI Digital object identifier for electronic documents.

10.1007/s10620-024-08334-8

URI

Authors

Wang H, Zhao D, Wang S, Liu H, Zhao S, Li Z, Qin X, Liu X

Journal

Digestive diseases and sciences

Year

2024

Keywords:

Constipation, Correlation networks, Diagnose, Fecal metabolomics, Intestinal microbiota

BACKGROUND: Constipation is one of the most common gastrointestinal complaints. Yet, the underlying mechanisms of constipation remain to be explored deeply. Integration of microbiome and metabolome is powerful and promising to demonstrate characteristics of constipation. AIM OF STUDY: This study aimed to characterize intestinal microbiome and metabolome of constipation. In addition, this study revealed the correlations among behaviors, intestinal microbiota, and metabolites interrupted by constipation. METHODS: Firstly, the constipation model was successfully applied. At the macro level, the ability of learning, memory, locomotor activity, and the defecation index of rats with constipation-like phenotype were characterized. At the micro-level, 16S rRNA sequencing was applied to analyze the intestinal microbiota in rats with constipation-like phenotype. 1H nuclear magnetic resonance (NMR)-based metabolomics was employed to investigate the metabolic phenotype of constipation. In addition, we constructed a correlation network, intuitively showing the correlations among behaviors, intestinal microbiota, and metabolites. RESULTS: Constipation significantly attenuated the locomotor activity, memory recognition, and frequency of defecation of rats, while increased the time of defecation. Constipation significantly changed the diversity of intestinal microbial communities, which correspondingly involved in 5 functional pathways. Besides, 28 fecal metabolites were found to be associated with constipation, among which 14 metabolites were further screened that can be used to diagnose constipation. On top of this, associated networks intuitively showed the correlations among behaviors, intestinal microbiota, and metabolites. CONCLUSIONS: The current findings are significant in terms of not only laying a foundation for understanding characteristics of constipation, but also providing accurate diagnosis and treatments of constipation clinically.

Experiment 1

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Curated date: 2025/03/20

Curator: Thelee98

Revision editor(s): Thelee98

Subjects

Location of subjects: China

Host species Species from which microbiome was sampled. Contact us to have more species added.: Rattus norvegicus

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Constipation Constipation,Costiveness,Dyschezia,constipation

Group 0 name Corresponds to the control (unexposed) group for case-control studies: Negative Control

Group 1 name Corresponds to the case (exposed) group for case-control studies: Constipation Group

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: The Group 1 (constipation Group) were defined as Sprague–Dawley male rats that had a constipation-like phenotype induced by intragastrical administration of white vinegar and activated carbon-cold water for 5 weeks. The diagnostic Criteria are: 1. Longer gastrointestinal transit time: Rats with constipation-like phenotype took significantly longer to complete gastrointestinal propulsion compared to controls. 2. Reduced small intestinal propulsion rate: The propulsion rate of the small intestine was significantly lower in constipated rats. 3. Decreased defecation frequency and volume: The frequency and amount of defecation were significantly reduced, mimicking clinical constipation symptoms in humans.

Group 0 sample size Number of subjects in the control (unexposed) group: 6

Group 1 sample size Number of subjects in the case (exposed) group: 6

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V3-V4

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).: relative abundances

Statistical test: LEfSe

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: No

LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool: 2.0

Confounders controlled for Confounding factors that have been accounted for by stratification or model adjustment: body weight, Confounders controlled for: "food" is not in the list (abnormal glucose tolerance, acetaldehyde, acute graft vs. host disease, acute lymphoblastic leukemia, acute myeloid leukemia, adenoma, age, AIDS, alcohol consumption measurement, alcohol drinking, ...) of allowed values.food

Alpha Diversity

Pielou Quantifies how equal the community is numerically: increased

Shannon Estimator of species richness and species evenness: more weight on species richness: increased

Chao1 Abundance-based estimator of species richness: increased

Simpson Estimator of species richness and species evenness: more weight on species evenness: increased

Richness Number of species: increased

Signature 1

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Curated date: 2025/03/20

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Source: Figures 2B and 2D

Description: Represents the LDA Effect Size (LEfSe) analysis results comparing the NC and CG groups.

Abundance in Group 1: increased abundance in Constipation Group

NCBI	Quality Control	Links
Actinomycetota
Adlercreutzia
Anaerofustis
Bacilli
Bilophila
Brevundimonas
Caulobacteraceae
Caulobacterales
Clostridium
Coriobacteriaceae
Coriobacteriales
Coriobacteriia
Eubacteriaceae
Lactobacillaceae
Lactobacillales
Lactobacillus
Peptostreptococcaceae
Phascolarctobacterium
Ruminococcus
Turicibacter
Turicibacteraceae
rc4-4rc4-4
TM7-3TM7-3
TuricibacteralesTuricibacterales
CW040CW040
F16F16
SMB53SMB53
peptstreptoccacea clostridiumpeptstreptoccacea clostridium
saccharibacteriasaccharibacteria