Gastrointestinal Characteristics of Constipation from the Perspectives of Microbiome and Metabolome
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Study information
-
Quality control
- Retracted paper
- Contamination issues suspected
- Batch effect issues suspected
- Uncontrolled confounding suspected
- Results are suspect (various reasons)
- Tags applied
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI Uniform resource identifier for web resources.
Authors
Wang H, Zhao D, Wang S, Liu H, Zhao S, Li Z, Qin X, Liu X
Journal
Digestive diseases and sciences
Year
2024
Keywords:
Constipation, Correlation networks, Diagnose, Fecal metabolomics, Intestinal microbiota
BACKGROUND: Constipation is one of the most common gastrointestinal complaints. Yet, the underlying mechanisms of constipation remain to be explored deeply. Integration of microbiome and metabolome is powerful and promising to demonstrate characteristics of constipation. AIM OF STUDY: This study aimed to characterize intestinal microbiome and metabolome of constipation. In addition, this study revealed the correlations among behaviors, intestinal microbiota, and metabolites interrupted by constipation. METHODS: Firstly, the constipation model was successfully applied. At the macro level, the ability of learning, memory, locomotor activity, and the defecation index of rats with constipation-like phenotype were characterized. At the micro-level, 16S rRNA sequencing was applied to analyze the intestinal microbiota in rats with constipation-like phenotype. 1H nuclear magnetic resonance (NMR)-based metabolomics was employed to investigate the metabolic phenotype of constipation. In addition, we constructed a correlation network, intuitively showing the correlations among behaviors, intestinal microbiota, and metabolites. RESULTS: Constipation significantly attenuated the locomotor activity, memory recognition, and frequency of defecation of rats, while increased the time of defecation. Constipation significantly changed the diversity of intestinal microbial communities, which correspondingly involved in 5 functional pathways. Besides, 28 fecal metabolites were found to be associated with constipation, among which 14 metabolites were further screened that can be used to diagnose constipation. On top of this, associated networks intuitively showed the correlations among behaviors, intestinal microbiota, and metabolites. CONCLUSIONS: The current findings are significant in terms of not only laying a foundation for understanding characteristics of constipation, but also providing accurate diagnosis and treatments of constipation clinically.
Experiment 1
Reviewed Marked as Reviewed by KateRasheed on 2025-4-29
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Rattus norvegicus
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Constipation Constipation,Costiveness,Dyschezia,constipation
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Negative Control
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Constipation Group
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- The Constipation Group were Sprague–Dawley male rats that had a constipation-like phenotype induced by intragastrical administration of white vinegar and activated carbon-cold water for 5 weeks. The diagnostic Criteria are: 1. Longer gastrointestinal transit time: Rats with constipation-like phenotype took significantly longer to complete gastrointestinal propulsion compared to controls. 2. Reduced small intestinal propulsion rate: The propulsion rate of the small intestine was significantly lower in constipated rats. 3. Decreased defecation frequency and volume: The frequency and amount of defecation were significantly reduced, mimicking clinical constipation symptoms in humans.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 4
- Group 1 sample size Number of subjects in the case (exposed) group
- 4
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- LEfSe
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 2
Alpha Diversity
- Pielou Quantifies how equal the community is numerically
- increased
- Shannon Estimator of species richness and species evenness: more weight on species richness
- increased
- Chao1 Abundance-based estimator of species richness
- increased
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- increased
- Richness Number of species
- increased
Signature 1
Reviewed Marked as Reviewed by KateRasheed on 2025-4-29
Source: Figures 2B and 2D
Description: Represents the LDA Effect Size (LEfSe) analysis results comparing the Negative Control and Constipation Group.
Abundance in Group 1: increased abundance in Constipation Group
| NCBI | Quality Control | Links |
|---|---|---|
| Adlercreutzia | ||
| Bilophila | ||
| Lachnospiraceae | ||
| Phascolarctobacterium | ||
| Ruminococcus | ||
| Veillonellaceae | ||
| RC4-4RC4-4 |
Signature 2
Reviewed Marked as Reviewed by KateRasheed on 2025-4-29
Source: Figures 2B and 2D
Description: Represents the LDA Effect Size (LEfSe) analysis results comparing the Negative Control and Constipation Group.
Abundance in Group 1: decreased abundance in Constipation Group
Revision editor(s): Thelee98, MyleeeA, KateRasheed
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