Oral microbiome dysbiosis among cigarette smokers and smokeless tobacco users compared to non-users/Experiment 13
From BugSigDB
Reviewed Marked as Reviewed by KateRasheed on 2025-7-31
Subjects
- Location of subjects
- United States of America
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Saliva , Buccal mucosa Sailva normalis,Saliva atomaris,Saliva molecularis,Salivary gland secretion,Saliva,saliva,Buccal mucosa,buccal mucosa
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Specimen Specimen,specimen
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Non-users (NU - Buccal)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Non-users (NU - Saliva)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Saliva samples of participants who had either never used tobacco products or had smoked less than 20 cigarettes or used smokeless tobacco less than 20 times in their lifetime.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 43
- Group 1 sample size Number of subjects in the case (exposed) group
- 43
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- 6 months
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- Kruskall-Wallis
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
Signature 1
Reviewed Marked as Reviewed by KateRasheed on 2025-7-31
Source: Supplementary Figure 9
Description: Box plots of relative abundance of the top 14 bacterial genera by sample type (buccal swab (red), saliva (blue) across each user group: cigarette users (CG), smokeless tobacco users (ST), and non-users (NU).
Abundance in Group 1: increased abundance in Non-users (NU - Saliva)
| NCBI | Quality Control | Links |
|---|---|---|
| Leptotrichia | ||
| Neisseria | ||
| Porphyromonas | ||
| Actinomyces | ||
| Prevotella |
Signature 2
Reviewed Marked as Reviewed by KateRasheed on 2025-7-31
Source: Supplementary Figure 9
Description: Box plots of relative abundance of the top 14 bacterial genera by sample type (buccal swab (red), saliva (blue) across each user group: cigarette users (CG), smokeless tobacco users (ST), and non-users (NU).
Abundance in Group 1: decreased abundance in Non-users (NU - Saliva)
| NCBI | Quality Control | Links |
|---|---|---|
| Lactobacillus | ||
| Veillonella | ||
| Streptococcus | ||
| Rothia | ||
| Pseudomonas | ||
| Granulicatella |
