Oral microbiome dysbiosis among cigarette smokers and smokeless tobacco users compared to non-users/Experiment 6

From BugSigDB


Needs review

Curated date: 2025/04/30

Curator: Shulamite

Revision editor(s): Shulamite

Subjects

Location of subjects
United States of America
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Buccal epithelium Buccal epithelium,buccal epithelium
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Smoking behaviour measurement Smoking behaviour measurement,smoking behaviour measurement
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Smokeless tobacco user (ST).
Group 1 name Corresponds to the case (exposed) group for case-control studies
Non-tobacco users (NU)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Users who had never used a tobacco product in their lifetime.
Group 0 sample size Number of subjects in the control (unexposed) group
18
Group 1 sample size Number of subjects in the case (exposed) group
43
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
6 Months

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
Kruskall-Wallis
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
Matched on Factors on which subjects have been matched on in a case-control study
age, race, sex

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
decreased

Signature 1

Needs review

Curated date: 2025/04/30

Curator: Shulamite

Revision editor(s): Shulamite

Source: Fig 5B

Description: Relative abundance of bacterial OTUs in buccal swab samples that were statistically significantly different (α = 0.001) between (a) non-users (NU) and cigarette users (CG) and (b) non-users (NU) and smokeless tobacco users (ST).

Abundance in Group 1: increased abundance in Non-tobacco users (NU)

NCBI Quality ControlLinks
uncultured Streptococcus sp.
uncultured Actinomyces sp.
uncultured Mogibacteriaceaeuncultured Mogibacteriaceae
uncultured Oribacterium sp.
uncultured Peptostreptococcus sp.

Revision editor(s): Shulamite

Signature 2

Needs review

Curated date: 2025/04/30

Curator: Shulamite

Revision editor(s): Shulamite

Source: Fig 5B

Description: Relative abundance of bacterial OTUs in buccal swab samples that were statistically significantly different (α = 0.001) between (a) non-users (NU) and cigarette users (CG) and (b) non-users (NU) and smokeless tobacco users (ST).

Abundance in Group 1: decreased abundance in Non-tobacco users (NU)

NCBI Quality ControlLinks
uncultured Neisseria sp.
uncultured Achromobacter sp.
Neisseria subflava
Haemophilus parainfluenzae

Revision editor(s): Shulamite