Oral microbiota of patients with phenylketonuria: A nation-based cross-sectional study/Experiment 8

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Reviewed Marked as Reviewed by Svetlana up on 2025-1-15

Curated date: 2024/07/31

Curator: MyleeeA

Revision editor(s): MyleeeA, Tosin, WikiWorks

Subjects

Location of subjects

Germany

Host species Species from which microbiome was sampled. Contact us to have more species added.

Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology

SalivaSaliva

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology

PhenylketonuriaPhenylketonuria

Group 0 name Corresponds to the control (unexposed) group for case-control studies

ChCtrlRel-S [related control children saliva (Healthy siblings of PKU patients)]

Group 1 name Corresponds to the case (exposed) group for case-control studies

ChPKU-S (PKU children saliva) patients

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group

Children diagnosed with Phenylketonuria (PKU) and who stimulated Saliva samples were gotten from.

Group 0 sample size Number of subjects in the control (unexposed) group

12

Group 1 sample size Number of subjects in the case (exposed) group

59

Lab analysis

Sequencing type

16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene

V1-V2

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance

Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).

relative abundances

Statistical test

Mann-Whitney (Wilcoxon)

Significance threshold p-value or FDR threshold used for differential abundance testing (if any)

0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?

Yes

Matched on Factors on which subjects have been matched on in a case-control study

age

Alpha Diversity

Pielou Quantifies how equal the community is numerically

unchanged

Shannon Estimator of species richness and species evenness: more weight on species richness

unchanged

Richness Number of species

unchanged

Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample

unchanged