The Role of Gut Microbiota in Male Erectile Dysfunction of Rats
From BugSigDB
Jump to:navigation, search
Study information
-
Quality control
- Retracted paper
- Contamination issues suspected
- Batch effect issues suspected
- Uncontrolled confounding suspected
- Results are suspect (various reasons)
- Tags applied
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
Xu Z, Wang S, Liu C, Kang J, Pan Y, Zhang Z, Zhou H, Xu M, Li X, Wang H, Niu S, Liu L, Sun D, Liu X
Journal
The world journal of men's health
Year
2025
Keywords:
Diet, high-fat, Erectile dysfunction, Gastrointestinal microbiome, Inflammation, Metabolomics
PURPOSE: Erectile dysfunction (ED) is a common male sexual dysfunction. Gut microbiota plays an important role in various diseases. To investigate the effects and mechanisms of intestinal flora dysregulation induced by high-fat diet (HFD) on erectile function. MATERIALS AND METHODS: Male Sprague-Dawley rats aged 8 weeks were randomly divided into the normal diet (ND) and HFD groups. After 24 weeks, a measurement of erectile function was performed. We performed 16S rRNA sequencing of stool samples. Then, we established fecal microbiota transplantation (FMT) rat models by transplanting fecal microbiota from rats of ND group and HFD group to two new groups of rats respectively. After 24 weeks, erectile function of the rats was evaluated and 16S rRNA sequencing was performed, and serum samples were collected for the untargeted metabolomics detection. RESULTS: The erectile function of rats and the species diversity of intestinal microbiota in the HFD group was significantly lower, and the characteristics of the intestinal microbiota community structure were also significantly different between the two groups. The erectile function of rats in the HFD-FMT group was significantly lower than that of rats in the ND-FMT group. The characteristics of the intestinal microbiota community structure were significantly different. In the HFD-FMT group, 27 metabolites were significantly different and they were mainly involved in the several inflammation-related pathways. CONCLUSIONS: Intestinal microbiota disorders induced by HFD can damage the intestinal barrier of rats, change the serum metabolic profile, induce low-grade inflammation and apoptosis in the corpus cavernosum of the penis, and lead to ED.
Experiment 1
Reviewed Marked as Reviewed by Svetlana up on 2025-3-24
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Rattus norvegicus
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Response to diet Response to diet,response to diet
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Normal Diet (ND)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Long-term high-fat diet (HFD)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- The mice in the high-fat diet (HFD) groups were fed a HFD (carbohydrate, 20%; protein, 20%; fat, 60%; Research Diets). The treatment lasted for 24 weeks
- Group 0 sample size Number of subjects in the control (unexposed) group
- 6
- Group 1 sample size Number of subjects in the case (exposed) group
- 6
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- Mann-Whitney (Wilcoxon)
- T-Test
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Chao1 Abundance-based estimator of species richness
- decreased
- Richness Number of species
- decreased
- Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
- decreased
Signature 1
Reviewed Marked as Reviewed by Svetlana up on 2025-3-24
Source: Figure 2K
Description: Comparison of genus abundance between Normal Diet and High Fat Diet groups.
Abundance in Group 1: increased abundance in Long-term high-fat diet (HFD)
| NCBI | Quality Control | Links |
|---|---|---|
| Desulfovibrio |
Revision editor(s): MyleeeA
Signature 4
Reviewed Marked as Reviewed by Svetlana up on 2025-3-24
Source: Figure 2 (H and J)
Description: Comparison of genus abundance between Normal Diet and High Fat Diet groups.
Abundance in Group 1: decreased abundance in Long-term high-fat diet (HFD)
| NCBI | Quality Control | Links |
|---|---|---|
| Bacteroidota | ||
| Lactobacillus |
Revision editor(s): MyleeeA
Experiment 3
Reviewed Marked as Reviewed by Svetlana up on 2025-3-24
Differences from previous experiment shown
Subjects
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Response to transplant Response to transplant,response to transplant
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Normal Diet-Fecal Microbiota Transplantation (ND-FMT)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- High-fat diet-Fecal Microbiota Transplantation (HFD-FMT)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- The mice in the High Fat Diet Fecal Microbiota Transplantation (HFD-FMT) group were fed fecal microbiota liquid prepared from the feces of rats on HFD.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 5
- Group 1 sample size Number of subjects in the case (exposed) group
- 5
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Chao1 Abundance-based estimator of species richness
- unchanged
- Richness Number of species
- unchanged
- Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
- unchanged
Signature 1
Reviewed Marked as Reviewed by Svetlana up on 2025-3-24
Source: Figure 4 G, H and I
Description: Genus analysis of intestinal microbiota in Normal Diet FMT and High Fat Diet FMT.
Abundance in Group 1: increased abundance in High-fat diet-Fecal Microbiota Transplantation (HFD-FMT)
| NCBI | Quality Control | Links |
|---|---|---|
| Clostridium | ||
| Desulfovibrio | ||
| Mucispirillum |
Revision editor(s): MyleeeA
Signature 2
Reviewed Marked as Reviewed by Svetlana up on 2025-3-24
Source: Figure 4 J, K, L and M
Description: Genus analysis of intestinal microbiota in Normal Diet FMT and High Fat Diet FMT.
Abundance in Group 1: decreased abundance in High-fat diet-Fecal Microbiota Transplantation (HFD-FMT)
| NCBI | Quality Control | Links |
|---|---|---|
| Lactobacillus | ||
| Pediococcus | ||
| Odoribacter | ||
| Helicobacter |
Revision editor(s): MyleeeA
Experiment 4
Reviewed Marked as Reviewed by Svetlana up on 2025-3-24
Differences from previous experiment shown
Subjects
Lab analysis
Statistical Analysis
- Statistical test
- LEfSe
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Chao1 Abundance-based estimator of species richness
- unchanged
- Richness Number of species
- unchanged
- Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
- unchanged
Signature 1
Reviewed Marked as Reviewed by Svetlana up on 2025-3-24
Source: Figure 4F
Description: Differential analysis of intestinal microbiota in Normal Diet FMT and High Fat Diet FMT.
Abundance in Group 1: increased abundance in High-fat diet-Fecal Microbiota Transplantation (HFD-FMT)
Revision editor(s): MyleeeA
Signature 2
Reviewed Marked as Reviewed by Svetlana up on 2025-3-24
Source: Figure 4F
Description: Differential analysis of intestinal microbiota in Normal Diet FMT and High Fat Diet FMT.
Abundance in Group 1: decreased abundance in High-fat diet-Fecal Microbiota Transplantation (HFD-FMT)
| NCBI | Quality Control | Links |
|---|---|---|
| Lactobacillales | ||
| Lactobacillus | ||
| Bacilli | ||
| Lactobacillaceae | ||
| Pediococcus | ||
| Helicobacter | ||
| Odoribacter | ||
| Odoribacteraceae |
Revision editor(s): MyleeeA
Retrieved from "https://bugsigdb.org/w/index.php?title=38772541&oldid=190830"
