The impact of small intestinal bacterial overgrowth on the efficacy of fecal microbiota transplantation in patients with chronic constipation/Experiment 1

From BugSigDB


Reviewed Marked as Reviewed by Svetlana up on 2025-3-24

Curated date: 2025/03/21

Curator: Miss Lulu

Revision editor(s): Miss Lulu

Subjects

Location of subjects
China
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Response to transplant Response to transplant,response to transplant
Group 0 name Corresponds to the control (unexposed) group for case-control studies
PosF0 - Fecal sample in Small intestinal bacterial overgrowth group (SIBO group) at pre-FMT
Group 1 name Corresponds to the case (exposed) group for case-control studies
PosF7 - Fecal sample in Small intestinal bacterial overgrowth group (SIBO group) seven days after FMT
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Small intestinal bacterial overgrowth (SIBO) group seven days after FMT
Group 0 sample size Number of subjects in the control (unexposed) group
88
Group 1 sample size Number of subjects in the case (exposed) group
88
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
Extensive antibiotic use

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
-V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
LEfSe
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
2

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged

Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2025-3-24

Curated date: 2025/03/21

Curator: Miss Lulu

Revision editor(s): Miss Lulu

Source: Figure 3A

Description: Represents the LEfSe analysis of the colon microbiota and small intestinal microbiota for two groups of patients.

Abundance in Group 1: increased abundance in PosF7 - Fecal sample in Small intestinal bacterial overgrowth group (SIBO group) seven days after FMT

NCBI Quality ControlLinks
Bacteroidaceae
Bacteroidales
Bacteroidia
Bacteroidota
Bacteroides

Revision editor(s): Miss Lulu

Signature 2

Reviewed Marked as Reviewed by Svetlana up on 2025-3-24

Curated date: 2025/03/21

Curator: Miss Lulu

Revision editor(s): Miss Lulu

Source: Figure 3A

Description: Represents the LEfSe analysis of the colon microbiota and small intestinal microbiota for two groups of patients.

Abundance in Group 1: decreased abundance in PosF7 - Fecal sample in Small intestinal bacterial overgrowth group (SIBO group) seven days after FMT

NCBI Quality ControlLinks
Enterobacterales
Enterobacteriaceae
Gammaproteobacteria
Pseudomonadota
Escherichia/Shigella sp.

Revision editor(s): Miss Lulu