The respiratory microbiome is linked to the severity of RSV infections and the persistence of symptoms in children/Experiment 1
From BugSigDB
Reviewed Marked as Reviewed by Svetlana up on 2025-4-23
Subjects
- Location of subjects
- Belgium
- Finland
- Netherlands
- Spain
- United Kingdom
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Nasopharynx Epipharynx,Nasal part of pharynx,Nasenrachenraum,Pars nasalis pharyngis,Rhinopharynx,Nasopharynx,nasopharynx
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Respiratory Syncytial Virus Infection Human respiratory syncytial virus infection,infections, Human respiratory syncytial virus,respiratory syncytial virus infection,respiratory syncytial virus infectious disease,Respiratory Syncytial Virus Infection,respiratory Syncytial Virus Infection
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Healthy Controls
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- RSV (Respiratory Syncytial Virus) Infection
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Infants with RSV (Respiratory Syncytial Virus) infection during the first year of life.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 52
- Group 1 sample size Number of subjects in the case (exposed) group
- 374
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- log transformation
- Statistical test
- MaAsLin2
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
- Confounders controlled for Confounding factors that have been accounted for by stratification or model adjustment
- age, Confounders controlled for: "gender" is not in the list (abnormal glucose tolerance, acetaldehyde, acute graft vs. host disease, acute lymphoblastic leukemia, acute myeloid leukemia, adenoma, age, AIDS, alcohol consumption measurement, alcohol drinking, ...) of allowed values.gender, Confounders controlled for: "study site" is not in the list (abnormal glucose tolerance, acetaldehyde, acute graft vs. host disease, acute lymphoblastic leukemia, acute myeloid leukemia, adenoma, age, AIDS, alcohol consumption measurement, alcohol drinking, ...) of allowed values.study site
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
Signature 1
Reviewed Marked as Reviewed by Svetlana up on 2025-4-23
Source: Fig 2E-F
Description: Taxonomic differences of nasopharyngeal microbiota in infants with RSV infection versus healthy controls.
Abundance in Group 1: increased abundance in RSV (Respiratory Syncytial Virus) Infection
| NCBI | Quality Control | Links |
|---|---|---|
| Haemophilus | ||
| Moraxella |
Revision editor(s): AmyUchay, PreciousChijioke
Signature 2
Reviewed Marked as Reviewed by Svetlana up on 2025-4-23
Source: Fig 2E-F
Description: Taxonomic differences of nasopharyngeal microbiota in infants with RSV infection versus healthy controls.
Abundance in Group 1: decreased abundance in RSV (Respiratory Syncytial Virus) Infection
| NCBI | Quality Control | Links |
|---|---|---|
| Corynebacterium | ||
| Dolosigranulum | ||
| Dolosigranulum pigrum |
Revision editor(s): AmyUchay, PreciousChijioke
