Dysbiosis of infant gut microbiota is related to the altered fatty acid composition of human milk from mothers with gestational diabetes mellitus: a prospective cohort study/Experiment 4

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Reviewed Marked as Reviewed by Svetlana up on 2025-3-26

Curated date: 2025/03/26

Curator: Tosin

Revision editor(s): Tosin

Subjects

Location of subjects
China
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Milk Mammary gland milk,Milk,milk
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Gestational diabetes diabetes in pregnancy,GDM,gestational diabetes,gestational diabetes mellitus,maternal gestational diabetes mellitus,Gestational diabetes
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Normal group 1 (1 month postpartum)
Group 1 name Corresponds to the case (exposed) group for case-control studies
Gestational diabetes mellitus group 1 (GDM) (1 month postpartum)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Pregnant women with GDM (gestational diabetes mellitus) 1 month postpartum
Group 0 sample size Number of subjects in the control (unexposed) group
40
Group 1 sample size Number of subjects in the case (exposed) group
40
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
3 months

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
Linear Regression
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
Yes
Confounders controlled for Confounding factors that have been accounted for by stratification or model adjustment
birth weight, body mass index, Confounders controlled for: "birth length" is not in the list (abnormal glucose tolerance, acetaldehyde, acute graft vs. host disease, acute lymphoblastic leukemia, acute myeloid leukemia, adenoma, age, AIDS, alcohol consumption measurement, alcohol drinking, ...) of allowed values.birth length, Confounders controlled for: "maternal dietary carbohydrate" is not in the list (abnormal glucose tolerance, acetaldehyde, acute graft vs. host disease, acute lymphoblastic leukemia, acute myeloid leukemia, adenoma, age, AIDS, alcohol consumption measurement, alcohol drinking, ...) of allowed values.maternal dietary carbohydrate, Confounders controlled for: "dietary fibre intake" is not in the list (abnormal glucose tolerance, acetaldehyde, acute graft vs. host disease, acute lymphoblastic leukemia, acute myeloid leukemia, adenoma, age, AIDS, alcohol consumption measurement, alcohol drinking, ...) of allowed values.dietary fibre intake

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged
Chao1 Abundance-based estimator of species richness
increased
Simpson Estimator of species richness and species evenness: more weight on species evenness
unchanged
Richness Number of species
increased

Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2025-3-26

Curated date: 2025/03/26

Curator: Tosin

Revision editor(s): Tosin

Source: Table 3

Description: Differential bacteria between GDM and normal groups with a p-time×group interaction < 0.05.

Abundance in Group 1: increased abundance in Gestational diabetes mellitus group 1 (GDM) (1 month postpartum)

NCBI Quality ControlLinks
Candidatus Altimarinota
Sphingomonas
unclassified Sphingobacteriaceae
Allorhizobium_Neorhizobium_Pararhizobium_RhizobiumAllorhizobium_Neorhizobium_Pararhizobium_Rhizobium
unclassified Candidatus Saccharimonadota
Unclassified LWQ8Unclassified LWQ8
unclassified Paracoccaceae

Revision editor(s): Tosin