The difference of oropharyngeal microbiome during acute respiratory viral infections in infants and children/Experiment 1
From BugSigDB
Reviewed Marked as Reviewed by KateRasheed on 2025-6-13
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Oropharynx Mesopharynx,Oral part of pharynx,Pars oralis pharyngis,Oropharynx,oropharynx
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Respiratory tract infectious disease infection, respiratory tract,infections, respiratory,infections, respiratory tract,infections, upper respiratory,infections, upper respiratory tract,respiratory infection, upper,respiratory infections,respiratory tract infection,upper respiratory infections,upper respiratory tract infections,Respiratory tract infectious disease,respiratory tract infectious disease
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Inpatients
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Outpatients
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Children with Acute respiratory infections (ARI) who visited the hospital but did not require hospitalization.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 49
- Group 1 sample size Number of subjects in the case (exposed) group
- 60
Lab analysis
- Sequencing type
- WMS
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- Not specified
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- LEfSe
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 2
- Matched on Factors on which subjects have been matched on in a case-control study
- age, sex
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Richness Number of species
- unchanged
Signature 1
Reviewed Marked as Reviewed by KateRasheed on 2025-6-13
Source: Fig. 2F and G
Description: LDA score and relative abundance of 15 significantly different taxa calculated by LEfSe analysis.
Abundance in Group 1: increased abundance in Outpatients
Revision editor(s): Shulamite
