Effects of vaginal microbiota on in vitro fertilization outcomes in women with different infertility causes/Experiment 4

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Reviewed Marked as Reviewed by Svetlana up on 2025-4-8

Curated date: 2025/03/31

Curator: Victoria

Revision editor(s): Victoria

Subjects

Location of subjects
China
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Vaginal fluid Vaginal discharge,Vaginal secretion,Vaginal fluid,vaginal fluid
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Female infertility female infertility,female reproductive system infertility,female reproductive system infertility disorder,female sterility,female sub-fertility,female subfertility,infertility disorder of female reproductive system,postpartum sterility,sterility, female,sterility, postpartum,sub fertility, female,sub-fertility, female,subfertility, female,Female infertility
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Polycystic Ovary Syndrome (PCOS) group
Group 1 name Corresponds to the case (exposed) group for case-control studies
Tubal factor infertility (TFI) group
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
This group consists of women with tubal factor infertility.
Group 0 sample size Number of subjects in the control (unexposed) group
32
Group 1 sample size Number of subjects in the case (exposed) group
45

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
T-Test
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged
Simpson Estimator of species richness and species evenness: more weight on species evenness
unchanged

Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2025-4-8

Curated date: 2025/03/31

Curator: Victoria

Revision editor(s): Victoria

Source: Figure 4B

Description: t-test analysis of different microbes at the species and genus levels.

Abundance in Group 1: increased abundance in Tubal factor infertility (TFI) group

NCBI Quality ControlLinks
Ligilactobacillus
Lactobacillus iners

Revision editor(s): Victoria

Signature 2

Reviewed Marked as Reviewed by Svetlana up on 2025-4-8

Curated date: 2025/03/31

Curator: Victoria

Revision editor(s): Victoria

Source: Figure 4B

Description: t-test analysis of different microbes at the species and genus levels.

Abundance in Group 1: decreased abundance in Tubal factor infertility (TFI) group

NCBI Quality ControlLinks
Dialister

Revision editor(s): Victoria