Effects of vaginal microbiota on in vitro fertilization outcomes in women with different infertility causes/Experiment 6
From BugSigDB
Reviewed Marked as Reviewed by Svetlana up on 2025-4-8
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Vaginal fluid Vaginal discharge,Vaginal secretion,Vaginal fluid,vaginal fluid
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Female infertility female infertility,female reproductive system infertility,female reproductive system infertility disorder,female sterility,female sub-fertility,female subfertility,infertility disorder of female reproductive system,postpartum sterility,sterility, female,sterility, postpartum,sub fertility, female,sub-fertility, female,subfertility, female,Female infertility
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Tubal Factor Infertility.Pregnant (TFI.P) group
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Tubal Factor Infertility.Non-pregnant (TFI.NP) group
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- This group consists of infertile women with Tubal Factor Infertility who are not pregnant.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 21
- Group 1 sample size Number of subjects in the case (exposed) group
- 24
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- LEfSe
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 2
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- unchanged
Signature 1
Reviewed Marked as Reviewed by Svetlana up on 2025-4-8
Source: Figure 5E
Description: Histogram of taxa with differential abundance computed using LDA scores.
Abundance in Group 1: increased abundance in Tubal Factor Infertility.Non-pregnant (TFI.NP) group
| NCBI | Quality Control | Links |
|---|---|---|
| Pseudomonadaceae | ||
| Pseudomonas | ||
| Gemelliphila asaccharolytica | ||
| Ralstonia | ||
| Lysobacteraceae |
Revision editor(s): Victoria
Signature 2
Reviewed Marked as Reviewed by Svetlana up on 2025-4-8
Source: Figure 5E
Description: Histogram of taxa with differential abundance computed using LDA scores.
Abundance in Group 1: decreased abundance in Tubal Factor Infertility.Non-pregnant (TFI.NP) group
| NCBI | Quality Control | Links |
|---|---|---|
| Helicobacter typhlonius | ||
| Porphyromonas endodontalis | ||
| Actinomycetaceae | ||
| Ureaplasma parvum | ||
| Actinomyces | ||
| Megasphaera | ||
| Allobaculum | ||
| Scardovia | ||
| Scardovia wiggsiae | ||
| Blautia |
Revision editor(s): Victoria
