Different Efficacy of Five Soluble Dietary Fibers on Alleviating Loperamide-Induced Constipation in Mice: Influences of Different Structural Features/Experiment 4

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Reviewed Marked as Reviewed by KateRasheed on 2025-5-5

Curated date: 2025/04/24

Curator: Miss Lulu

Revision editor(s): Miss Lulu

Subjects

Location of subjects
China
Host species Species from which microbiome was sampled. Contact us to have more species added.
Mus musculus
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Caecum Blind intestine,Blindgut,Caeca,Caecum,Ceca,Cecum,Intestinum caecum,Intestinum crassum caecum,Intestinum crassum cecum,caecum
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Response to diet Response to diet,response to diet
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Model control group (MC)
Group 1 name Corresponds to the case (exposed) group for case-control studies
Positive treatment group (PT)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Male ICR mice administered to positive treatments, the corresponding soluble fibers at doses of 400 mg/kg bw/d
Group 0 sample size Number of subjects in the control (unexposed) group
12
Group 1 sample size Number of subjects in the case (exposed) group
12

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
Kruskall-Wallis
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged
Chao1 Abundance-based estimator of species richness
unchanged

Signature 1

Reviewed Marked as Reviewed by KateRasheed on 2025-5-5

Curated date: 2025/04/24

Curator: Miss Lulu

Revision editor(s): Miss Lulu

Source: Figures 4C,D,F,G,J

Description: Relative Abundance of genera in the gut microbiota of mice

Abundance in Group 1: increased abundance in Positive treatment group (PT)

NCBI Quality ControlLinks
unclassified Desulfovibrionaceae
Oscillibacter
Ruminococcus
unclassified Oscillospiraceae

Revision editor(s): Miss Lulu