Roseburia hominis improves host metabolism in diet-induced obesity/Experiment 1
From BugSigDB
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Mus musculus
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Obesity , High fat diet Adiposis,Adiposity,Obese,Obese (finding),obesity,Obesity (disorder),Obesity [Ambiguous],obesity disease,obesity disorder,Obesity NOS,Obesity, unspecified,Overweight and obesity,Obesity,HF - High fat diet,High fat diet (finding),High fat diet,high fat diet
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- High Fat Diet-Phosphate-buffered saline (HFD-PBS)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- High Fat Diet- Roseburia hominis (HFD-RH))
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Mice were fed high-fat diet (40% fat), and orally challenged with 10⁹ colony forming units of R. hominis
- Group 0 sample size Number of subjects in the control (unexposed) group
- 5
- Group 1 sample size Number of subjects in the case (exposed) group
- 5
Lab analysis
- Sequencing type
- WMS
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- Not specified
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- LEfSe
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 2
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
Signature 1
Source: Figure 4c and Figure 4d
Description: LDA effect size at the genus and species level between HFD-RH and HFD-PBS group by LEfSe
Abundance in Group 1: increased abundance in High Fat Diet- Roseburia hominis (HFD-RH))
Revision editor(s): Z.uk.a
Signature 2
Source: Figure 4c and Figure 4d
Description: LDA effect size at the genus and species level between HFD-RH and HFD-PBS group by LEfSe
Abundance in Group 1: decreased abundance in High Fat Diet- Roseburia hominis (HFD-RH))
Revision editor(s): Z.uk.a
