Smoking-related gut microbiota alteration is associated with obesity and obesity-related diseases: results from two cohorts with sibling comparison analyses

Study information

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Reviewed Marked as Reviewed by KateRasheed on 2025-6-20

study design

cross-sectional observational, not case-control

Citation

PMID PubMed identifier for scientific articles.

40059170

DOI Digital object identifier for electronic documents.

https://doi.org/10.1186/s12916-025-03969-4

URI Uniform resource identifier for web resources.

Authors

Duan Y, Xu C, Wang W, Wang X, Xu N, Zhong J, Gong W, Zheng W, Wu YH, Myers A, Chu L, Lu Y, Delzell E, Hsing AW, Yu M, He W, Zhu S

Journal

BMC medicine

Year

2025

Keywords:

Gut microbiota, Obesity, Obesity-related disease, Smoking

BACKGROUND: Individuals who smoke tend to have a lower body mass index (BMI) but face an increased risk of obesity-related diseases. This study investigates this paradox from the perspective of gut microbiota. METHODS: We conducted microbiome analyses to identify smoking-related microbial genera and created a smoking-related microbiota index (SMI) using 16S rRNA sequencing data from 4000 male participants in WELL-China cohort and Lanxi cohort. We employed logistic regression to explore the association between SMI and obesity indices derived from dual-energy X-ray absorptiometry. Cox regression analyses were conducted to explore the association of SMI with incident of obesity-related diseases. To further control for unmeasured familial confounders, sibling comparison analyses were conducted using between-within (BW) model. RESULTS: The smoking-related microbiota index (SMI) showed a positive association with BMI and other obesity indices. Further analyses revealed that SMI is linked to obesity-related diseases, with hazard ratios (95% confidence intervals) of 1.97 (1.41-2.75) for incident diabetes, 1.31 (1.01-1.71) for major adverse cardiovascular events, and 1.70 (1.05-2.75) for obesity-related cancers. Results from sibling comparison analyses reinforced these findings. CONCLUSIONS: While smoking may reduce weight through various mechanisms, alterations in gut microbiota related to smoking are associated with weight gain. Further research is required to determine if changes in the smoking-related microbiome contribute to weight gain following smoking cessation.

Experiment 1

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Reviewed Marked as Reviewed by KateRasheed on 2025-6-20

Curated date: 2025/04/23

Curator: PreciousChijioke

Revision editor(s): PreciousChijioke, Anne-mariesharp

Subjects

Location of subjects

China

Host species Species from which microbiome was sampled. Contact us to have more species added.

Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology

Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology

Body mass index BMI,Quetelet's Index,Body mass index,body mass index

Group 0 name Corresponds to the control (unexposed) group for case-control studies

Low BMI (Body Mass Index) (WELL-China cohort)

Group 1 name Corresponds to the case (exposed) group for case-control studies

High BMI (Body Mass Index) (WELL-China cohort)

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group

Individuals with high body mass index in the WELL-China cohort (the discovery cohort)

Lab analysis

Sequencing type

16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene

V3-V4

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance

Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).

arcsine square-root

Statistical test

MaAsLin2

Significance threshold p-value or FDR threshold used for differential abundance testing (if any)

0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?

Yes

Signature 1

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Reviewed Marked as Reviewed by KateRasheed on 2025-6-20

Curated date: 2025/04/23

Curator: PreciousChijioke

Revision editor(s): PreciousChijioke, Anne-mariesharp

Source: Fig. 1B (C2)

Description: Smoking-related microbial genera and obesity in the WELL-China cohort

Abundance in Group 1: decreased abundance in High BMI (Body Mass Index) (WELL-China cohort)

NCBI	Quality Control	Links
Bacteria incertae sedis
Lachnospiraceae bacterium NK4A136
Ruminococcaceae_UCG-013Ruminococcaceae_UCG-013

Revision editor(s): PreciousChijioke, Anne-mariesharp

Experiment 2

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Curated date: 2025/04/23

Curator: PreciousChijioke

Revision editor(s): PreciousChijioke, Anne-mariesharp

Differences from previous experiment shown

Subjects

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology

Body fat percentage Body fat percentage,body fat percentage

Group 0 name Corresponds to the control (unexposed) group for case-control studies

Low body fat (WELL-China cohort)

Group 1 name Corresponds to the case (exposed) group for case-control studies

High body fat (WELL-China cohort)

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group

Individuals with high body fat percentage in the WELL-China cohort (the discovery cohort)

Lab analysis

Statistical Analysis

Signature 1

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Curated date: 2025/04/23

Curator: PreciousChijioke

Revision editor(s): PreciousChijioke, Anne-mariesharp

Source: Fig. 1B (C2)

Description: Smoking-related microbial genera and obesity in the WELL-China cohort

Abundance in Group 1: decreased abundance in High body fat (WELL-China cohort)

NCBI	Quality Control	Links
Bacteria incertae sedis
Lachnospiraceae bacterium NK4A136
Ruminococcaceae_UCG-013Ruminococcaceae_UCG-013

Revision editor(s): PreciousChijioke, Anne-mariesharp

Experiment 3

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Reviewed Marked as Reviewed by KateRasheed on 2025-6-20

Curated date: 2025/04/24

Curator: PreciousChijioke

Revision editor(s): PreciousChijioke, Anne-mariesharp

Differences from previous experiment shown

Subjects

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology

Waist circumference Waist circumference,waist circumference

Group 0 name Corresponds to the control (unexposed) group for case-control studies

Low WC (Waist circumference) (WELL-China cohort)

Group 1 name Corresponds to the case (exposed) group for case-control studies

High WC (Waist circumference) (WELL-China cohort)

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group

Individuals with high waist circumference in the WELL-China cohort (the discovery cohort)

Lab analysis

Statistical Analysis

Signature 2

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Curated date: 2025/04/24

Curator: PreciousChijioke

Revision editor(s): PreciousChijioke, Anne-mariesharp

Source: Fig 1B (C2)

Description: Smoking-related microbial genera and obesity in the WELL-China cohort

Abundance in Group 1: decreased abundance in High WC (Waist circumference) (WELL-China cohort)

NCBI	Quality Control	Links
Ruminococcaceae_UCG-013Ruminococcaceae_UCG-013
Bacteria incertae sedis
Lachnospiraceae bacterium NK4A136

Revision editor(s): PreciousChijioke, Anne-mariesharp

Experiment 4

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Reviewed Marked as Reviewed by KateRasheed on 2025-6-20

Curated date: 2025/04/24

Curator: PreciousChijioke

Revision editor(s): PreciousChijioke, Anne-mariesharp

Differences from previous experiment shown

Subjects

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology

Waist-hip ratio Waist-hip ratio,waist-hip ratio

Group 0 name Corresponds to the control (unexposed) group for case-control studies

Low WHR(Waist-hip ratio) (WELL-China cohort)

Group 1 name Corresponds to the case (exposed) group for case-control studies

High WHR(Waist-hip ratio) (WELL-China cohort)

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group

Individuals with high waist-hip ratio in the WELL-China cohort (the discovery cohort)

Lab analysis

Statistical Analysis

Signature 1

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Reviewed Marked as Reviewed by KateRasheed on 2025-6-20

Curated date: 2025/04/24

Curator: PreciousChijioke

Revision editor(s): PreciousChijioke, Anne-mariesharp

Source: Fig 1B (C2)

Description: Smoking-related microbial genera and obesity in the WELL-China cohort

Abundance in Group 1: decreased abundance in High WHR(Waist-hip ratio) (WELL-China cohort)

NCBI	Quality Control	Links
Bacteria incertae sedis
Ruminococcaceae_UCG-013Ruminococcaceae_UCG-013
Lachnospiraceae bacterium NK4A136

Revision editor(s): PreciousChijioke, Anne-mariesharp

Experiment 5

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Curated date: 2025/04/24

Curator: PreciousChijioke

Revision editor(s): PreciousChijioke, Anne-mariesharp

Differences from previous experiment shown

Subjects

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology

Smoking status measurement Smoking status measurement,smoking status measurement

Group 0 name Corresponds to the control (unexposed) group for case-control studies

Never smokers of the WELL-China cohort

Group 1 name Corresponds to the case (exposed) group for case-control studies

Current smokers of the WELL-China cohort

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group

Current smokers of the WELL-China cohort (the discovery cohort)

Group 0 sample size Number of subjects in the control (unexposed) group

1126

Group 1 sample size Number of subjects in the case (exposed) group

1143

Lab analysis

Statistical Analysis

Matched on Factors on which subjects have been matched on in a case-control study

Matched on: "sibling pair" is not in the list (abnormal glucose tolerance, acetaldehyde, acute graft vs. host disease, acute lymphoblastic leukemia, acute myeloid leukemia, adenoma, age, AIDS, alcohol consumption measurement, alcohol drinking, ...) of allowed values.sibling pair

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness

unchanged

Simpson Estimator of species richness and species evenness: more weight on species evenness

unchanged

Richness Number of species

unchanged

Signature 1

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Curated date: 2025/04/24

Curator: PreciousChijioke

Revision editor(s): PreciousChijioke, Anne-mariesharp

Source: Fig. 1B (C1) & Table S2

Description: Smoking-related microbial genera identified in male participants of the WELL-China cohort.

Abundance in Group 1: increased abundance in Current smokers of the WELL-China cohort

NCBI	Quality Control	Links
Actinomyces
Actinomycetaceae
Actinomycetales
Actinomycetota
Atopobium
Bacillati
Bacilli
Clostridia
Coriobacteriaceae
Coriobacteriales
Coriobacteriia
Erysipelotrichaceae
Erysipelotrichales
Erysipelotrichia
Eubacteriales
Gammaproteobacteria
Gemella
Haemophilus
Lachnospiraceae
Oscillospiraceae
Pasteurellaceae
Pasteurellales
Pseudomonadota
Solobacterium
Tyzzerella
Ruminococcaceae_UCG.013Ruminococcaceae_UCG.013
Lachnospiraceae bacterium NK4A136
Bacteria incertae sedis

Revision editor(s): PreciousChijioke, Anne-mariesharp

Signature 2

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Curated date: 2025/04/27

Curator: PreciousChijioke

Revision editor(s): PreciousChijioke, Anne-mariesharp

Source: Fig. 1B (C1) & Table S2

Description: Smoking-related microbial genera identified in male participants of the WELL-China cohort.

Abundance in Group 1: decreased abundance in Current smokers of the WELL-China cohort

NCBI	Quality Control	Links
Achromobacter
Acidaminococcaceae
Acidaminococcus
Actinomycetota
Alcaligenaceae
Allisonella
Anaerosporobacter
Bacillati
Bacilli
Bacteria incertae sedis
Bacteroidales
Bacteroidia
Bacteroidota
Betaproteobacteria
Bilophila
Burkholderiales
Candidatus Saccharimonadota
Clostridia
Collinsella
Coprobacillus
Coriobacteriaceae
Coriobacteriales
Coriobacteriia
Deltaproteobacteria
Desulfovibrionaceae
Desulfovibrionales
Enterobacterales
Enterobacteriaceae
Erysipelotrichaceae
Erysipelotrichales
Erysipelotrichia
Escherichia
Eubacteriales
Eubacterium
Faecalitalea
Fusobacteriaceae
Fusobacteriia
Fusobacteriota
Fusobacterium
Gammaproteobacteria
Gemella
Haemophilus
Hungatella
Kluyvera
Lachnoclostridium
Lachnospira
Lachnospiraceae
Lachnospiraceae bacterium NK4A136
Lactobacillales
Lactobacillus
Megasphaera
Mollicutes
Mycoplasmatota
Negativicutes
Oscillospiraceae
Oxalobacteraceae
Parabacteroides
Parasutterella
Pasteurellaceae
Pasteurellales
Porphyromonadaceae
Pseudomonadota
Ruminococcus gauvreauii
Selenomonadales
Shigella
Slackia
Subdoligranulum
Sutterella
Veillonellaceae
Weissella
[Eubacterium] nodatum
Fusobacteriales CFT112H7Fusobacteriales CFT112H7
Ruminococcaceae_UCG.004Ruminococcaceae_UCG.004
Christensenellaceae_R.7_groupChristensenellaceae_R.7_group
Ruminococcaceae_UCG.014Ruminococcaceae_UCG.014
Lachnospiraceae_UCG.001Lachnospiraceae_UCG.001
Ruminococcaceae_UCG.003Ruminococcaceae_UCG.003
Lachnospiraceae_ND3007_groupLachnospiraceae_ND3007_group
Mollicutes_RF9Mollicutes_RF9
Ruminococcaceae_UCG.013Ruminococcaceae_UCG.013
Lactobacillaceae
Eubacterium ventriosum
Eubacterium ruminantium
Christensenellaceae
Coprococcus

Revision editor(s): PreciousChijioke, Anne-mariesharp

Experiment 6

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Curated date: 2025/04/24

Curator: PreciousChijioke

Revision editor(s): PreciousChijioke, Anne-mariesharp

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies

Never smokers of the Lanxi cohort

Group 1 name Corresponds to the case (exposed) group for case-control studies

Current smokers of the Lanxi cohort

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group

Current smokers of the Lanxi cohort (the replication cohort)

Group 0 sample size Number of subjects in the control (unexposed) group

646

Group 1 sample size Number of subjects in the case (exposed) group

471

Lab analysis

Statistical Analysis

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness

unchanged

Simpson Estimator of species richness and species evenness: more weight on species evenness

unchanged

Richness Number of species

unchanged

Signature 1

EditHistoryDelete

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Reviewed Marked as Reviewed by KateRasheed on 2025-6-20

Curated date: 2025/04/27

Curator: PreciousChijioke

Revision editor(s): PreciousChijioke, Anne-mariesharp

Source: Fig 1B (D1) & Table S2

Description: Smoking-related microbial genera identified in male participants of the Lanxi cohort.

Abundance in Group 1: increased abundance in Current smokers of the Lanxi cohort

NCBI	Quality Control	Links
Actinomyces
Actinomycetaceae
Actinomycetales
Actinomycetota
Atopobium
Bacilli
Clostridia
Coriobacteriaceae
Coriobacteriales
Coriobacteriia
Erysipelotrichaceae
Erysipelotrichales
Erysipelotrichia
Eubacteriales
Gammaproteobacteria
Haemophilus
Lachnospiraceae
Oscillospiraceae
Pasteurellaceae
Pasteurellales
Pseudomonadota
Solobacterium
Tyzzerella
Ruminococcaceae_UCG.013Ruminococcaceae_UCG.013
Bacteria incertae sedis
Lachnospiraceae bacterium NK4A136
Gemella

Revision editor(s): PreciousChijioke, Anne-mariesharp

Signature 2

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Reviewed Marked as Reviewed by KateRasheed on 2025-6-20

Curated date: 2025/04/27

Curator: PreciousChijioke

Revision editor(s): PreciousChijioke, Anne-mariesharp

Source: Fig 1B (D1) & Table S2

Description: Smoking-related microbial genera identified in male participants of the Lanxi cohort.

Abundance in Group 1: decreased abundance in Current smokers of the Lanxi cohort

NCBI	Quality Control	Links
Actinomycetota
Akkermansia
Bacteroidales
Bacteroidia
Bacteroidota
Barnesiella
Cetobacterium
Citrobacter
Clostridia
Coriobacteriaceae
Coriobacteriales
Coriobacteriia
Enterobacterales
Enterobacteriaceae
Erysipelotrichaceae
Erysipelotrichales
Erysipelotrichia
Eubacteriales
Flavonifractor
Fusobacteriaceae
Fusobacteriales
Fusobacteriia
Fusobacteriota
Gammaproteobacteria
Oscillospiraceae
Porphyromonadaceae
Prevotellaceae
Pseudomonadota
Slackia
Turicibacter
Verrucomicrobiaceae
Verrucomicrobiales
Verrucomicrobiia
Verrucomicrobiota
Gemella
Haemophilus
Ruminiclostridium
Prevotella

Revision editor(s): PreciousChijioke, Anne-mariesharp

Experiment 7

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Reviewed Marked as Reviewed by KateRasheed on 2025-6-20

Curated date: 2025/06/18

Curator: Anne-mariesharp

Revision editor(s): Anne-mariesharp

Differences from previous experiment shown

Subjects

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology

Body fat distribution body fat patterning,Body fat distribution,body fat distribution

Group 0 name Corresponds to the control (unexposed) group for case-control studies

Low Android (WELL-China cohort)

Group 1 name Corresponds to the case (exposed) group for case-control studies

High Android (WELL-China cohort)

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group

Individuals with high android fat mass in the WELL-China cohort (the discovery cohort)

Group 0 sample size Number of subjects in the control (unexposed) group

Not specified

Group 1 sample size Number of subjects in the case (exposed) group

Not specified

Lab analysis

Statistical Analysis

Matched on Factors on which subjects have been matched on in a case-control study

Not specified

Signature 1

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Reviewed Marked as Reviewed by KateRasheed on 2025-6-20

Curated date: 2025/06/18

Curator: Anne-mariesharp

Revision editor(s): Anne-mariesharp

Source: Fig. 1B (C2)

Description: Smoking-related microbial genera and obesity in the WELL-China cohort

Abundance in Group 1: decreased abundance in High Android (WELL-China cohort)

NCBI	Quality Control	Links
Ruminococcaceae_UCG-013Ruminococcaceae_UCG-013
Bacteria incertae sedis
Lachnospiraceae bacterium NK4A136

Revision editor(s): Anne-mariesharp

Experiment 8

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Curated date: 2025/06/18

Curator: Anne-mariesharp

Revision editor(s): Anne-mariesharp

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies

Low Gynoid (WELL-China cohort)

Group 1 name Corresponds to the case (exposed) group for case-control studies

High Gynoid (WELL-China cohort)

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group

Individuals with high gynoid fat mass in the WELL-China cohort (the discovery cohort)

Lab analysis

Statistical Analysis

Signature 1

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Reviewed Marked as Reviewed by KateRasheed on 2025-6-20

Curated date: 2025/06/18

Curator: Anne-mariesharp

Revision editor(s): Anne-mariesharp

Source: Fig 1B (C2)

Description: Smoking-related microbial genera and obesity in the WELL-China cohort

Abundance in Group 1: increased abundance in High Gynoid (WELL-China cohort)

NCBI	Quality Control	Links
Bacteria incertae sedis
Ruminococcaceae_UCG-013Ruminococcaceae_UCG-013
Lachnospiraceae bacterium NK4A136

Revision editor(s): Anne-mariesharp

Experiment 9

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Curated date: 2025/06/18

Curator: Anne-mariesharp

Revision editor(s): Anne-mariesharp

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies

Low AOI(Android-gynoid fat ratio) (WELL-China cohort)

Group 1 name Corresponds to the case (exposed) group for case-control studies

High AOI(Android-gynoid fat ratio) (WELL-China cohort)

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group

Individuals with high Android-gynoid fat ratio in the WELL-China cohort (the discovery cohort)

Lab analysis

Statistical Analysis

Signature 1

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Reviewed Marked as Reviewed by KateRasheed on 2025-6-20

Curated date: 2025/06/18

Curator: Anne-mariesharp

Revision editor(s): Anne-mariesharp

Source: Fig 1B (C2)

Description: Smoking-related microbial genera and obesity in the WELL-China cohort

Abundance in Group 1: decreased abundance in High AOI(Android-gynoid fat ratio) (WELL-China cohort)

NCBI	Quality Control	Links
Ruminococcaceae_UCG-013Ruminococcaceae_UCG-013
Bacteria incertae sedis
Lachnospiraceae bacterium NK4A136

Revision editor(s): Anne-mariesharp

Experiment 10

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Reviewed Marked as Reviewed by KateRasheed on 2025-6-20

Curated date: 2025/06/18

Curator: Anne-mariesharp

Revision editor(s): Anne-mariesharp

Differences from previous experiment shown

Subjects

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology

Body mass index BMI,Quetelet's Index,Body mass index,body mass index

Group 0 name Corresponds to the control (unexposed) group for case-control studies

Low BMI (Body Mass Index) (Lanxi cohort)

Group 1 name Corresponds to the case (exposed) group for case-control studies

High BMI (Body Mass Index) (Lanxi cohort)

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group

Individuals with high body mass index in the Lanxi cohort (the replication cohort)

Lab analysis

Statistical Analysis

Signature 1

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Reviewed Marked as Reviewed by KateRasheed on 2025-6-20

Curated date: 2025/06/18

Curator: Anne-mariesharp

Revision editor(s): Anne-mariesharp

Source: Fig 1B (D2)

Description: Smoking-related microbial genera and obesity in the Lanxi cohort

Abundance in Group 1: decreased abundance in High BMI (Body Mass Index) (Lanxi cohort)

NCBI	Quality Control	Links
Lachnospiraceae bacterium NK4A136

Revision editor(s): Anne-mariesharp

Experiment 11

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Reviewed Marked as Reviewed by KateRasheed on 2025-6-20

Curated date: 2025/06/18

Curator: Anne-mariesharp

Revision editor(s): Anne-mariesharp

Differences from previous experiment shown

Subjects

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology

Body fat percentage Body fat percentage,body fat percentage

Group 0 name Corresponds to the control (unexposed) group for case-control studies

Low body fat (Lanxi cohort)

Group 1 name Corresponds to the case (exposed) group for case-control studies

High body fat (Lanxi cohort)

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group

Individuals with high body fat percentage in the Lanxi cohort (the replication cohort)

Lab analysis

Statistical Analysis

Signature 1

EditHistoryDelete

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Reviewed Marked as Reviewed by KateRasheed on 2025-6-20

Curated date: 2025/06/18

Curator: Anne-mariesharp

Revision editor(s): Anne-mariesharp

Source: Fig 1B (D2)

Description: Smoking-related microbial genera and obesity in the Lanxi cohort

Abundance in Group 1: decreased abundance in High body fat (Lanxi cohort)

NCBI	Quality Control	Links
Bacteria incertae sedis
Ruminococcaceae_UCG-013Ruminococcaceae_UCG-013
Lachnospiraceae bacterium NK4A136

Revision editor(s): Anne-mariesharp

Experiment 12

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Reviewed Marked as Reviewed by KateRasheed on 2025-6-20

Curated date: 2025/06/18

Curator: Anne-mariesharp

Revision editor(s): Anne-mariesharp

Differences from previous experiment shown

Subjects

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology

Waist circumference Waist circumference,waist circumference

Group 0 name Corresponds to the control (unexposed) group for case-control studies

Low WC (Waist circumference) (Lanxi cohort)

Group 1 name Corresponds to the case (exposed) group for case-control studies

High WC (Waist circumference) (Lanxi cohort)

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group

Individuals with high waist circumference in the Lanxi cohort (the replication cohort)

Lab analysis

Statistical Analysis

Signature 1

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Reviewed Marked as Reviewed by KateRasheed on 2025-6-20

Curated date: 2025/06/18

Curator: Anne-mariesharp

Revision editor(s): Anne-mariesharp

Source: Fig 1B (D2)

Description: Smoking-related microbial genera and obesity in the Lanxi cohort

Abundance in Group 1: decreased abundance in High WC (Waist circumference) (Lanxi cohort)

NCBI	Quality Control	Links
Lachnospiraceae bacterium NK4A136

Revision editor(s): Anne-mariesharp

Experiment 13

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Reviewed Marked as Reviewed by KateRasheed on 2025-6-20

Curated date: 2025/06/18

Curator: Anne-mariesharp

Revision editor(s): Anne-mariesharp

Differences from previous experiment shown

Subjects

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology

Waist-hip ratio Waist-hip ratio,waist-hip ratio

Group 0 name Corresponds to the control (unexposed) group for case-control studies

Low WHR(Waist-hip ratio) (Lanxi cohort)

Group 1 name Corresponds to the case (exposed) group for case-control studies

High WHR(Waist-hip ratio) (Lanxi cohort)

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group

Individuals with high waist-hip ratio in the Lanxi cohort (the replication cohort)

Lab analysis

Statistical Analysis

Signature 1

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Reviewed Marked as Reviewed by KateRasheed on 2025-6-20

Curated date: 2025/06/18

Curator: Anne-mariesharp

Revision editor(s): Anne-mariesharp

Source: Fig 1B (D2)

Description: Smoking-related microbial genera and obesity in the Lanxi cohort

Abundance in Group 1: increased abundance in High WHR(Waist-hip ratio) (Lanxi cohort)

NCBI	Quality Control	Links
Atopobium
Actinomyces

Revision editor(s): Anne-mariesharp

Signature 2

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Your review change was submitted

Reviewed Marked as Reviewed by KateRasheed on 2025-6-20

Curated date: 2025/06/18

Curator: Anne-mariesharp

Revision editor(s): Anne-mariesharp

Source: Fig 1B (D2)

Description: Smoking-related microbial genera and obesity in the Lanxi cohort

Abundance in Group 1: decreased abundance in High WHR(Waist-hip ratio) (Lanxi cohort)

NCBI	Quality Control	Links
Lachnospiraceae bacterium NK4A136

Revision editor(s): Anne-mariesharp

Experiment 14

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Reviewed Marked as Reviewed by KateRasheed on 2025-6-20

Curated date: 2025/06/18

Curator: Anne-mariesharp

Revision editor(s): Anne-mariesharp

Differences from previous experiment shown

Subjects

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology

Body fat distribution body fat patterning,Body fat distribution,body fat distribution

Group 0 name Corresponds to the control (unexposed) group for case-control studies

Low Android (Lanxi cohort)

Group 1 name Corresponds to the case (exposed) group for case-control studies

High Android (Lanxi cohort)

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group

Individuals with high android fat mass in the Lanxi cohort (the replication cohort)

Lab analysis

Statistical Analysis

Signature 1

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Reviewed Marked as Reviewed by KateRasheed on 2025-6-20

Curated date: 2025/06/18

Curator: Anne-mariesharp

Revision editor(s): Anne-mariesharp

Source: Fig 1B (D2)

Description: Smoking-related microbial genera and obesity in the Lanxi cohort

Abundance in Group 1: decreased abundance in High Android (Lanxi cohort)

NCBI	Quality Control	Links
Haemophilus
Lachnospiraceae bacterium NK4A136
Ruminococcaceae_UCG-013Ruminococcaceae_UCG-013

Revision editor(s): Anne-mariesharp

Experiment 15

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Reviewed Marked as Reviewed by KateRasheed on 2025-6-20

Curated date: 2025/06/18

Curator: Anne-mariesharp

Revision editor(s): Anne-mariesharp

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies

Low Gynoid (Lanxi cohort)

Group 1 name Corresponds to the case (exposed) group for case-control studies

High Gynoid (Lanxi cohort)

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group

Individuals with high gynoid fat mass in the Lanxi cohort (the replication cohort)

Lab analysis

Statistical Analysis

Signature 1

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Reviewed Marked as Reviewed by KateRasheed on 2025-6-20

Curated date: 2025/06/18

Curator: Anne-mariesharp

Revision editor(s): Anne-mariesharp

Source: Fig 1B (D2)

Description: Smoking-related microbial genera and obesity in the Lanxi cohort

Abundance in Group 1: increased abundance in High Gynoid (Lanxi cohort)

NCBI	Quality Control	Links
Haemophilus
Lachnospiraceae bacterium NK4A136
Bacteria incertae sedis
Ruminococcaceae_UCG-013Ruminococcaceae_UCG-013

Revision editor(s): Anne-mariesharp

Experiment 16

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Reviewed Marked as Reviewed by KateRasheed on 2025-6-20

Curated date: 2025/06/18

Curator: Anne-mariesharp

Revision editor(s): Anne-mariesharp

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies

Low AOI(Android-gynoid fat ratio) (Lanxi cohort)

Group 1 name Corresponds to the case (exposed) group for case-control studies

High AOI(Android-gynoid fat ratio) (Lanxi cohort)

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group

Individuals with high Android-gynoid fat ratio in the Lanxi cohort (the replication cohort)

Lab analysis

Statistical Analysis

Signature 1

EditHistoryDelete

Flag for review

Your review change was submitted

Reviewed Marked as Reviewed by KateRasheed on 2025-6-20

Curated date: 2025/06/18

Curator: Anne-mariesharp

Revision editor(s): Anne-mariesharp

Source: Fig 1B (D2)

Description: Smoking-related microbial genera and obesity in the Lanxi cohort

Abundance in Group 1: decreased abundance in High AOI(Android-gynoid fat ratio) (Lanxi cohort)

NCBI	Quality Control	Links
Haemophilus
Lachnospiraceae bacterium NK4A136
Bacteria incertae sedis
Ruminococcaceae_UCG-013Ruminococcaceae_UCG-013

Revision editor(s): Anne-mariesharp