Smoking-related gut microbiota alteration is associated with obesity and obesity-related diseases: results from two cohorts with sibling comparison analyses/Experiment 1

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Reviewed Marked as Reviewed by KateRasheed on 2025-6-20

Curated date: 2025/04/23

Curator: PreciousChijioke

Revision editor(s): PreciousChijioke, Anne-mariesharp

Subjects

Location of subjects

China

Host species Species from which microbiome was sampled. Contact us to have more species added.

Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology

Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology

Body mass index BMI,Quetelet's Index,Body mass index,body mass index

Group 0 name Corresponds to the control (unexposed) group for case-control studies

Low BMI (Body Mass Index) (WELL-China cohort)

Group 1 name Corresponds to the case (exposed) group for case-control studies

High BMI (Body Mass Index) (WELL-China cohort)

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group

Individuals with high body mass index in the WELL-China cohort (the discovery cohort)

Lab analysis

Sequencing type

16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene

V3-V4

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance

Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).

arcsine square-root

Statistical test

MaAsLin2

Significance threshold p-value or FDR threshold used for differential abundance testing (if any)

0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?

Yes

Signature 1

EditHistoryDelete

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Your review change was submitted

Reviewed Marked as Reviewed by KateRasheed on 2025-6-20

Curated date: 2025/04/23

Curator: PreciousChijioke

Revision editor(s): PreciousChijioke, Anne-mariesharp

Source: Fig. 1B (C2)

Description: Smoking-related microbial genera and obesity in the WELL-China cohort

Abundance in Group 1: decreased abundance in High BMI (Body Mass Index) (WELL-China cohort)

NCBI	Quality Control	Links
Bacteria incertae sedis
Lachnospiraceae bacterium NK4A136
Ruminococcaceae_UCG-013Ruminococcaceae_UCG-013

Revision editor(s): PreciousChijioke, Anne-mariesharp