Seasonal variations in circulating endocannabinoidome mediators and gut microbiota composition in humans/Experiment 2
Subjects
- Location of subjects
- Canada
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Seasonality measurement Seasonality measurement,seasonality measurement
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Low Seasonal Changes
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- High Seasonal Changes
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Participants who were categorized to have been observed with higher composition of gut microbiota associated with seasonal changes in the circulating levels of NAEs (N-acyl-ethanolamines)
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- 3 months
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- LEfSe
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 1.5
Signature 1
Source: Figure 7D
Description: Linear discriminant analysis effect size (LEfSe) analysis identifying microbial taxa associated with seasonal changes in the dim 3 family groups and, consequently, with the circulating NAEs seasonal changes observed (Kruskal-Wallis tests p < 0.05 and LDA score >1.5)
Abundance in Group 1: increased abundance in High Seasonal Changes
Signature 2
Source: Figure 7D
Description: linear discriminant analysis effect size (LEfSe) analysis identifying microbial taxa associated with seasonal changes in the dim 3 family groups and, consequently, with the circulating NAEs seasonal changes observed (Kruskal-Wallis tests p < 0.05 and LDA score >1.5)
Abundance in Group 1: decreased abundance in High Seasonal Changes
| NCBI | Quality Control | Links |
|---|---|---|
| Acidaminococcus | ||
| Blautia | ||
| Dialister | ||
| Faecalibacterium | ||
| Lactobacillus | ||
| Ruminococcus_1Ruminococcus_1 |
Revision editor(s): Montana-D
