Integrative metagenomics and metabolomics reveal age-associated gut microbiota and metabolite alterations in a hamster model of COVID-19/Experiment 2

From BugSigDB


Needs review

Curated date: 2025/05/04

Curator: MyleeeA

Revision editor(s): MyleeeA

Subjects

Location of subjects
France
Host species Species from which microbiome was sampled. Contact us to have more species added.
Mesocricetus auratus
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Caecum Blind intestine,Blindgut,Caeca,Caecum,Ceca,Cecum,Intestinum caecum,Intestinum crassum caecum,Intestinum crassum cecum,caecum
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
SARS-CoV-2-related disease COVID-19-related disease,SARS-CoV-2-related disease,sARS-CoV-2-related disease
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Young and Aged D0 non-infected controls (Age effect)
Group 1 name Corresponds to the case (exposed) group for case-control studies
Young and Aged D7 Infected (Age effect)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Young (2 months old) and Aged (22 months old) SARS-CoV-2-infected hamsters at Day 7 with age effect.

Lab analysis

Sequencing type
WMS
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
Not specified
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
MaAsLin2
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
Yes

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged

Signature 1

Needs review

Curated date: 2025/05/04

Curator: MyleeeA

Revision editor(s): MyleeeA

Source: Figure 3C

Description: Differential abundance of taxa between Young and Aged D0 non-infected controls (Age effect) and Young and Aged D7 Infected (Age effect) hamsters analyzed with Maaslin2 (adjusted P-value < 0.05).

Abundance in Group 1: increased abundance in Young and Aged D7 Infected (Age effect)

NCBI Quality ControlLinks
Bacteroides
Bifidobacterium
Eggerthellaceae
Ligilactobacillus
Odoribacter
Rikenella
Streptococcus
NM07_P.09NM07_P.09
RIkenellaceae NOVRIkenellaceae NOV
Faecalibacterium

Revision editor(s): MyleeeA

Signature 2

Needs review

Curated date: 2025/05/04

Curator: MyleeeA

Revision editor(s): MyleeeA

Source: Figure 3C

Description: Differential abundance of taxa between Young and Aged D0 non-infected controls (Age effect) and Young and Aged D7 Infected (Age effect) hamsters analyzed with Maaslin2 (adjusted P-value < 0.05).

Abundance in Group 1: decreased abundance in Young and Aged D7 Infected (Age effect)

NCBI Quality ControlLinks
Acetatifactor
Anaerostipes
Bacilli
Blautia
Eisenbergiella
Eubacterium sp. CAG:115
Eubacterium sp. CAG:180
Firmicutes bacterium CAG:475
Firmicutes bacterium CAG:582
Firmicutes bacterium CAG:822
Frisingicoccus
Granulicatella
Kineothrix
Lachnospiraceae bacterium
Roseburia
Roseburia sp. CAG:303
Turicimonas
unclassified Lachnospiraceae
UMGS1071UMGS1071
Erysipelotrichaceae NOVErysipelotrichaceae NOV
Helicobacter DHelicobacter D
Ruminococcus CRuminococcus C
UMGS1217UMGS1217
UBA7057UBA7057
Ruminiclostridium ERuminiclostridium E
Helicobacter AHelicobacter A
Helicobacter CHelicobacter C
Butyrivibrio AButyrivibrio A
CAG:518CAG:518

Revision editor(s): MyleeeA