Multiple sclerosis and gut microbiota: Lachnospiraceae from the ileum of MS twins trigger MS-like disease in germfree transgenic mice-An unbiased functional study/Experiment 3
From BugSigDB
Reviewed Marked as Reviewed by KateRasheed on 2025-6-24
Subjects
- Location of subjects
- Germany
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Mus musculus
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Experimental autoimmune encephalomyelitis ALLERGIC ENCEPH,ALLERGIC ENCEPH EXPER,Allergic encephalomyelitis,Allergic encephalomyelitis (disorder),Allergic Encephalomyelitis, Experimental,AUTOIMMUNE ENCEPH EXPER,Autoimmune Encephalomyelitis, Experimental,AUTOIMMUNE EXPER ENCEPH,Autoimmune Experimental Encephalomyelitis,EAE,ENCEPH ALLERGIC,ENCEPH AUTOIMMUNE EXPER,ENCEPH EXPER AUTOIMMUNE,Encephalomyelitis, Allergic,Encephalomyelitis, Autoimmune Experimental,Encephalomyelitis, Autoimmune, Experimental,Encephalomyelitis, Experimental Allergic,Encephalomyelitis, Experimental Autoimmune,EXPER ALLERGIC ENCEPH,EXPER AUTOIMMUNE ENCEPH,Experimental Allergic Encephalomyelitides,Experimental Allergic Encephalomyelitis,Experimental allergic encephalomyelitis (disorder),experimental autoimmune encephalomyelitis,Experimental Encephalomyelitis, Autoimmune,Experimental autoimmune encephalomyelitis
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Healthy
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- EAE (Experimental Autoimmune Encephalomyelitis)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Gnotobiotic mice that developed Experimental Autoimmune Encephalomyelitis after being colonized with ileal microbiota from an MS (Multiple Sclerosis)-affected human twin (MS-Q).
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- T-Test
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- decreased
- Richness Number of species
- decreased
Signature 1
Reviewed Marked as Reviewed by KateRasheed on 2025-6-24
Source: Figure 3F
Description: Relative abundance of fecal microbiota in recipients of MS patient-derived ileal microbiota that showed a significant difference (unpaired t-test) between healthy and diseased animals.
Abundance in Group 1: increased abundance in EAE (Experimental Autoimmune Encephalomyelitis)
| NCBI | Quality Control | Links |
|---|---|---|
| Alcaligenes | ||
| Eisenbergiella |
Revision editor(s): Anne-mariesharp
Signature 2
Reviewed Marked as Reviewed by KateRasheed on 2025-6-24
Source: Figure 3F
Description: Relative abundance of fecal microbiota in recipients of MS patient-derived ileal microbiota that showed a significant difference (unpaired t-test) between healthy and diseased animals.
Abundance in Group 1: decreased abundance in EAE (Experimental Autoimmune Encephalomyelitis)
| NCBI | Quality Control | Links |
|---|---|---|
| Akkermansia | ||
| Thomasclavelia | ||
| unclassified Bacillota |
Revision editor(s): Anne-mariesharp
