The Urinary Microbiome in Women Using Single-Use Versus Reusable Catheters for Intermittent Catheterization: An Exploratory Substudy of the COMPaRE Trial/Experiment 1
Subjects
- Location of subjects
- Netherlands
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Lower urinary tract Lower urinary tract,lower urinary tract
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Urinary retention Increased post-void residual urine volume,Urinary retention,urinary retention
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Control arm / Single-use group
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Intervention arm / Reusable group
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Patients using reusable catheters (Cliny or PureCath brands) cleaned and stored in 1:80 dilution of 2% sodium hypochlorite, with each catheter used for 2 weeks
- Group 0 sample size Number of subjects in the control (unexposed) group
- 16
- Group 1 sample size Number of subjects in the case (exposed) group
- 12
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- Antibiotic treatment in the past 3 months (except for prophylaxis) Additional note: "Patients with missing samples or those receiving antibiotics during follow‐up were excluded" from certain analyses
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V1-V3
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- DESeq2
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.01
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
- Matched on Factors on which subjects have been matched on in a case-control study
- Matched on: "Randomized trial design" is not in the list (abnormal glucose tolerance, acetaldehyde, acute graft vs. host disease, acute lymphoblastic leukemia, acute myeloid leukemia, adenoma, age, AIDS, alcohol consumption measurement, alcohol drinking, ...) of allowed values.Randomized trial design, Matched on: "Baseline characteristics balanced between groups" is not in the list (abnormal glucose tolerance, acetaldehyde, acute graft vs. host disease, acute lymphoblastic leukemia, acute myeloid leukemia, adenoma, age, AIDS, alcohol consumption measurement, alcohol drinking, ...) of allowed values.Baseline characteristics balanced between groups, Matched on: "Paired samples (same patients at Week 0 and Week 6)" is not in the list (abnormal glucose tolerance, acetaldehyde, acute graft vs. host disease, acute lymphoblastic leukemia, acute myeloid leukemia, adenoma, age, AIDS, alcohol consumption measurement, alcohol drinking, ...) of allowed values.Paired samples (same patients at Week 0 and Week 6)
- Confounders controlled for Confounding factors that have been accounted for by stratification or model adjustment
- age, Confounders controlled for: "BMI (Body Mass Index)" is not in the list (abnormal glucose tolerance, acetaldehyde, acute graft vs. host disease, acute lymphoblastic leukemia, acute myeloid leukemia, adenoma, age, AIDS, alcohol consumption measurement, alcohol drinking, ...) of allowed values.BMI (Body Mass Index), Confounders controlled for: "Menopausal status" is not in the list (abnormal glucose tolerance, acetaldehyde, acute graft vs. host disease, acute lymphoblastic leukemia, acute myeloid leukemia, adenoma, age, AIDS, alcohol consumption measurement, alcohol drinking, ...) of allowed values.Menopausal status, Confounders controlled for: "CIC (Clean Intermittent Catheterization) frequency" is not in the list (abnormal glucose tolerance, acetaldehyde, acute graft vs. host disease, acute lymphoblastic leukemia, acute myeloid leukemia, adenoma, age, AIDS, alcohol consumption measurement, alcohol drinking, ...) of allowed values.CIC (Clean Intermittent Catheterization) frequency, Confounders controlled for: "Catheterization cause (neurogenic vs non-neurogenic)" is not in the list (abnormal glucose tolerance, acetaldehyde, acute graft vs. host disease, acute lymphoblastic leukemia, acute myeloid leukemia, adenoma, age, AIDS, alcohol consumption measurement, alcohol drinking, ...) of allowed values.Catheterization cause (neurogenic vs non-neurogenic)
Alpha Diversity
- Pielou Quantifies how equal the community is numerically
- unchanged
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Chao1 Abundance-based estimator of species richness
- unchanged
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- unchanged
- Inverse Simpson Modification of Simpsons index D as 1/D to obtain high values in datasets of high diversity and vice versa
- unchanged
- Richness Number of species
- unchanged
- Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
- unchanged
Signature 1
Source: Figure 5
Description: Opportunistic bacterial taxa significantly increased in women using reusable versus single-use catheters for clean intermittent self-catheterization. Veillonella, while found in healthy urinary microbiomes, has been associated with bladder cancer and stress urinary incontinence in other studies. Finegoldia magna, commonly detected in asymptomatic women, has been linked to worsening urinary symptoms before pelvic surgery. Study involved 28 female patients (67.9% neurogenic dysfunction) with 6-week follow-up, analyzed by 16S rRNA sequencing and DESeq2 differential abundance analysis (p<0.01).
Abundance in Group 1: increased abundance in Intervention arm / Reusable group
| NCBI | Quality Control | Links |
|---|---|---|
| Veillonella (p < 0.001)Veillonella (p < 0.001) | ||
| Finegoldia (p < 0.001)Finegoldia (p < 0.001) |
Revision editor(s): Martha KJ
Signature 2
Source: Figure 5
Description: Potential uropathogens significantly reduced in women transitioning to reusable catheters for clean intermittent self-catheterization. Escherichia-Shigella, the most abundant genus overall (36.3% prevalence), and Aerococcus both showed marked decreases, potentially indicating reduced urinary tract infection risk. However, the paper notes that E. coli presence may not be a reliable UTI predictor, as infections are influenced more by overall microbiome composition than specific pathogens. No significant difference in actual UTI rates was observed between groups during the study period. Study involved 28 female patients with neurogenic/non-neurogenic urinary retention, 6-week randomized controlled design.
Abundance in Group 1: decreased abundance in Intervention arm / Reusable group
| NCBI | Quality Control | Links |
|---|---|---|
| Aerococcus (p < 0.001)Aerococcus (p < 0.001) | ||
| Escherichia-Shigella (p < 0.001)Escherichia-Shigella (p < 0.001) |
Revision editor(s): Martha KJ
