Major urinary protein (Mup) gene family deletion drives sex-specific alterations on the house mouse gut microbiota/Experiment 1

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Curated date: 2024/03/19

Curator: Fetlework

Revision editor(s): Fetlework

Subjects

Location of subjects

United States of America

Host species Species from which microbiome was sampled. Contact us to have more species added.

Mus musculus

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology

Hindgut , Midgut , Foregut Metenteron,Hindgut,hindgut,Mesenteron,Midgut,midgut,Praeenteron,Proenteron,Foregut,foregut

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology

Gene deletion library gene knockdown library,Gene repression library,Gene silencing library,Gene deletion library,gene deletion library

Group 0 name Corresponds to the control (unexposed) group for case-control studies

male mice

Group 1 name Corresponds to the case (exposed) group for case-control studies

female mice

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group

female mice, including knock out and wild

Group 0 sample size Number of subjects in the control (unexposed) group

10

Group 1 sample size Number of subjects in the case (exposed) group

9

Lab analysis

Sequencing type

WMS

16S variable region One or more hypervariable region(s) of the bacterial 16S gene

Not specified

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance

MGISEQ-2000

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).

relative abundances

Statistical test

Mann-Whitney (Wilcoxon)

Mixed-Effects Regression

PERMANOVA

Significance threshold p-value or FDR threshold used for differential abundance testing (if any)

0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?

No

Alpha Diversity

Pielou Quantifies how equal the community is numerically

decreased

Shannon Estimator of species richness and species evenness: more weight on species richness

decreased

Richness Number of species

decreased