Current levels of microplastic pollution impact wild seabird gut microbiomes/Experiment 5

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Curated date: 2024/04/30

Curator: Bolanle

Revision editor(s): Bolanle

Subjects

Location of subjects

Canada

Host species Species from which microbiome was sampled. Contact us to have more species added.

Fulmarus glacialis

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology

Proventriculus , Cloaca Proventriculus,proventriculus,Cloacal chamber,Vent,Cloaca,cloaca

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology

Exposure Exposure,exposure

Group 0 name Corresponds to the control (unexposed) group for case-control studies

abundance of microplastic mass in proventriculus

Group 1 name Corresponds to the case (exposed) group for case-control studies

abundance of microplastic mass in cloaca

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group

effect of microplastic mass in f. glacialis

Group 0 sample size Number of subjects in the control (unexposed) group

85

Group 1 sample size Number of subjects in the case (exposed) group

84

Lab analysis

Sequencing type

16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene

V4

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance

Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).

centered log-ratio

Statistical test

ANCOM

Significance threshold p-value or FDR threshold used for differential abundance testing (if any)

0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?

Yes

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness

decreased

Richness Number of species

decreased

Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample

decreased