Current levels of microplastic pollution impact wild seabird gut microbiomes/Experiment 5
From BugSigDB
Subjects
- Location of subjects
- Canada
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Fulmarus glacialis
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Proventriculus , Cloaca Proventriculus,proventriculus,Cloacal chamber,Vent,Cloaca,cloaca
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Exposure Exposure,exposure
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- abundance of microplastic mass in proventriculus
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- abundance of microplastic mass in cloaca
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- effect of microplastic mass in f. glacialis
- Group 0 sample size Number of subjects in the control (unexposed) group
- 85
- Group 1 sample size Number of subjects in the case (exposed) group
- 84
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- centered log-ratio
- Statistical test
- ANCOM
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- decreased
- Richness Number of species
- decreased
- Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
- decreased