Maternal smoking during pregnancy increases the risk of gut microbiome-associated childhood overweight and obesity

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Reviewed Marked as Reviewed by Svetlana up on 2024-7-18
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
Peng Y, Tun HM, Ng SC, Wai HK, Zhang X, Parks J, Field CJ, Mandhane P, Moraes TJ, Simons E, Turvey SE, Subbarao P, Brook JR, Takaro TK, Scott JA, Chan FK, Kozyrskyj AL
Journal
Gut microbes
Year
2024
Keywords:
Maternal smoking, butyrate production, childhood obesity, gut microbiota
Childhood obesity is linked to maternal smoking during pregnancy. Gut microbiota may partially mediate this association and could be potential targets for intervention; however, its role is understudied. We included 1,592 infants from the Canadian Healthy Infants Longitudinal Development Cohort. Data on environmental exposure and lifestyle factors were collected prenatally and throughout the first three years. Weight outcomes were measured at one and three years of age. Stool samples collected at 3 and 12 months were analyzed by sequencing the V4 region of 16S rRNA to profile microbial compositions and magnetic resonance spectroscopy to quantify the metabolites. We showed that quitting smoking during pregnancy did not lower the risk of offspring being overweight. However, exclusive breastfeeding until the third month of age may alleviate these risks. We also reported that maternal smoking during pregnancy significantly increased Firmicutes abundance and diversity. We further revealed that Firmicutes diversity mediates the elevated risk of childhood overweight and obesity linked to maternal prenatal smoking. This effect possibly occurs through excessive microbial butyrate production. These findings add to the evidence that women should quit smoking before their pregnancies to prevent microbiome-mediated childhood overweight and obesity risk, and indicate the potential obesogenic role of excessive butyrate production in early life.

Experiment 1


Reviewed Marked as Reviewed by Svetlana up on 2024-7-18

Curated date: 2024/04/21

Curator: Omojokunoluwatomisin

Revision editor(s): Omojokunoluwatomisin, Scholastica

Subjects

Location of subjects
Canada
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Smoking behaviour measurement Smoking behaviour measurement,smoking behaviour measurement
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Early infants (3 months) with maternal history of 'NO' 'smoking
Group 1 name Corresponds to the case (exposed) group for case-control studies
Early infants (3 months) with maternal history of 'YES' 'smoking
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Infants (3 months old) whose mothers quit smoking during pregnancy, reduced the number of cigarettes, or had the same number of cigarettes (as before conception) during pregnancy.
Group 0 sample size Number of subjects in the control (unexposed) group
1458
Group 1 sample size Number of subjects in the case (exposed) group
134

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V4

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
LEfSe
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
Yes
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
2

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
increased
Chao1 Abundance-based estimator of species richness
increased
Simpson Estimator of species richness and species evenness: more weight on species evenness
increased
Richness Number of species
increased
Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
increased

Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2024-7-18

Curated date: 2024/04/21

Curator: Omojokunoluwatomisin

Revision editor(s): Omojokunoluwatomisin, Scholastica

Source: Figure 2B, Supplementary Table S8

Description: Differentially abundant taxa identified by LEfSe in smoke exposed versus non-smoke exposed early infants (3 months old)

Abundance in Group 1: increased abundance in Early infants (3 months) with maternal history of 'YES' 'smoking

NCBI Quality ControlLinks
Bacillota
Burkholderiales
Clostridia
Clostridioides
Eubacteriales
Flavonifractor
Lachnoclostridium
Lachnospiraceae
Lachnospirales
Mediterraneibacter gnavus
Megasphaera
Negativicutes
Oscillospiraceae
Oscillospiraceae incertae sedis
Peptostreptococcaceae
Peptostreptococcales
Selenomonadales
Sutterella
Sutterellaceae
Tissierellales
Veillonellaceae
Veillonellales
[Ruminococcus] torques
Ruminococcaceae UBA1819Ruminococcaceae UBA1819

Revision editor(s): Omojokunoluwatomisin, Scholastica

Signature 2

Reviewed Marked as Reviewed by Svetlana up on 2024-7-18

Curated date: 2024/04/21

Curator: Omojokunoluwatomisin

Revision editor(s): Omojokunoluwatomisin, Scholastica

Source: Figure 2B, Supplementary Table S8

Description: Differentially abundant taxa identified by LEfSe in smoke exposed versus non-smoke exposed early infants (3 months old)

Abundance in Group 1: decreased abundance in Early infants (3 months) with maternal history of 'YES' 'smoking

NCBI Quality ControlLinks
Blautia
Haemophilus
Micrococcaceae
Micrococcales
Oscillospiraceae
Pasteurellaceae
Pasteurellales
Rothia

Revision editor(s): Omojokunoluwatomisin, Scholastica

Experiment 2


Reviewed Marked as Reviewed by Svetlana up on 2024-7-18

Curated date: 2024/04/22

Curator: Omojokunoluwatomisin

Revision editor(s): Omojokunoluwatomisin, Scholastica

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
Late infants (12months) with maternal history of 'NO' 'smoking
Group 1 name Corresponds to the case (exposed) group for case-control studies
Late infants (12months) with maternal history of 'YES' 'smoking
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Infants (12 months old) whose mothers quit smoking during pregnancy, reduced the number of cigarettes, or had the same number of cigarettes (as before conception) during pregnancy.

Lab analysis

Statistical Analysis

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
increased
Chao1 Abundance-based estimator of species richness
increased
Simpson Estimator of species richness and species evenness: more weight on species evenness
increased
Richness Number of species
increased
Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
increased

Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2024-7-18

Curated date: 2024/04/22

Curator: Omojokunoluwatomisin

Revision editor(s): Omojokunoluwatomisin, Scholastica, MyleeeA

Source: Figure 2B, Supplementary Table S8

Description: Differentially abundant taxa identified by LEfSe in smoke exposed versus non-smoke exposed late infants (12 months old)

Abundance in Group 1: increased abundance in Late infants (12months) with maternal history of 'YES' 'smoking

NCBI Quality ControlLinks
Bilophila
Butyricicoccaceae
Butyricicoccus
Colidextribacter
Desulfovibrionaceae
Desulfovibrionales
Desulfovibrionia
Flavonifractor
Oscillospiraceae
Oscillospiraceae incertae sedis
Sutterella
Thermodesulfobacteriota
[Ruminococcus] torques
uncultured Lachnospiraceae bacterium
Ruminococcaceae UBA1819Ruminococcaceae UBA1819
Clostridia UCG 014Clostridia UCG 014
Coprobacillaceae

Revision editor(s): Omojokunoluwatomisin, Scholastica, MyleeeA

Signature 2

Reviewed Marked as Reviewed by Svetlana up on 2024-7-18

Curated date: 2024/04/22

Curator: Omojokunoluwatomisin

Revision editor(s): Omojokunoluwatomisin, Scholastica

Source: Figure 2B, Supplementary Table S8

Description: Differentially abundant taxa identified by LEfSe in smoke exposed versus non-smoke exposed late infants (12 months old)

Abundance in Group 1: decreased abundance in Late infants (12months) with maternal history of 'YES' 'smoking

NCBI Quality ControlLinks
Haemophilus
Lachnospira
Pasteurellaceae
Pasteurellales
Selenomonadales
Veillonella
Veillonellaceae

Revision editor(s): Omojokunoluwatomisin, Scholastica