Maternal smoking during pregnancy increases the risk of gut microbiome-associated childhood overweight and obesity
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Quality control
- Retracted paper
- Contamination issues suspected
- Batch effect issues suspected
- Uncontrolled confounding suspected
- Results are suspect (various reasons)
- Tags applied
Experiment 1
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Smoking behaviour measurement Smoking behaviour measurement,smoking behaviour measurement
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Early infants (3 months) with maternal history of 'NO' 'smoking
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Early infants (3 months) with maternal history of 'YES' 'smoking
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Infants with mothers who quit smoking during pregnancy reduced the number of cigarettes or had the same number of cigarettes (as before conception) during pregnancy.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 1458
- Group 1 sample size Number of subjects in the case (exposed) group
- 134
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Mass spectrometry
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- LEfSe
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- increased
- Chao1 Abundance-based estimator of species richness
- increased
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- increased
- Richness Number of species
- increased
- Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
- increased
Signature 1
Source: Figure 2b and Table S8
Description: Differentially abundant taxa identified by LEfSe. “Yes”: samples from infants whose mothers quit smoking during pregnancy, reduced the number of cigarettes or had the same number of cigarettes (as before conception) during pregnancy. “No”: samples from infants whose mothers quit smoking before conception, or never smoked.
Abundance in Group 1: increased abundance in Early infants (3 months) with maternal history of 'YES' 'smoking
Revision editor(s): Omojokunoluwatomisin
Signature 2
Source: Figure 2b and Table S8
Description: Differentially abundant taxa identified by LEfSe. “Yes”: samples from infants whose mothers quit smoking during pregnancy, reduced the number of cigarettes, or had the same number of cigarettes (as before conception) during pregnancy. “No”: samples from infants whose mothers quit smoking before conception, or never smoked.
Abundance in Group 1: decreased abundance in Early infants (3 months) with maternal history of 'YES' 'smoking
| NCBI | Quality Control | Links |
|---|---|---|
| Haemophilus | ||
| Micrococcaceae | ||
| Micrococcales | ||
| Oscillospiraceae | ||
| Pasteurellaceae | ||
| Pasteurellales | ||
| Rothia | ||
| Blautia |
Revision editor(s): Omojokunoluwatomisin
Experiment 2
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Late infants (12months) with maternal history of 'NO' 'smoking
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Late infants (12months) with maternal history of 'YES' 'smoking
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- increased
- Chao1 Abundance-based estimator of species richness
- increased
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- increased
- Richness Number of species
- increased
- Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
- increased
Signature 1
Source: Figure 2b and Table S8
Description: Differentially abundant taxa identified by LEfSe. “Yes”: samples from infants whose mothers quit smoking during pregnancy, reduced the number of cigarettes or had the same number of cigarettes (as before conception) during pregnancy. “No”: samples from infants whose mothers quit smoking before conception, or never smoked.
Abundance in Group 1: increased abundance in Late infants (12months) with maternal history of 'YES' 'smoking
Revision editor(s): Omojokunoluwatomisin
Signature 2
Source: Figure 2b and Table S8
Description: Differentially abundant taxa identified by LEfSe. “Yes”: samples from infants whose mothers quit smoking during pregnancy, reduced the number of cigarettes or had the same number of cigarettes (as before conception) during pregnancy. “No”: samples from infants whose mothers quit smoking before conception, or never smoked.
Abundance in Group 1: decreased abundance in Late infants (12months) with maternal history of 'YES' 'smoking
| NCBI | Quality Control | Links |
|---|---|---|
| Haemophilus | ||
| Pasteurellaceae | ||
| Pasteurellales | ||
| Veillonella | ||
| Veillonellaceae | ||
| Selenomonadales | ||
| Lachnospira |
Revision editor(s): Omojokunoluwatomisin
