Insights into estrogen impact in oral health & microbiome in COVID-19

From BugSigDB
Reviewed Marked as Reviewed by Svetlana up on 2024-6-21
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
Bayardo-González RA, Peña-Rodríguez M, Pereira-Suárez AL, Rubio-Sánchez AX, García-Chagollán M, Valenzuela-Orozco DN, Lizarazo-Taborda MDR, Mora-Mora J, Vega-Magaña N
Journal
BMC microbiology
Year
2024
Keywords:
COVID-19, Cytokines, Inflammation, Microbiome, Women-s health
BACKGROUND: COVID-19 emerged in late 2019 and has occasioned more than 765 millions cumulative cases and 6.9 millions of deaths globally. Notably, around 70% of patients with severe COVID-19 are men. Therefore, it is to be presumed that women have a hormonal protector factor in inflammation and ACE2 expression. On the other hand, oral health status, and local microbiome can be key factors to respiratory viral infections control. Nevertheless, it has been poorly investigated. In our study 20 premenopausal, 18 postmenopausal and 22 men with COVID-19 were included. Oral health status, viral load, lingual ACE2 expression, as well as microbiome, estrogens and cytokines in saliva were analyzed. RESULTS: Our results showed a lower expression of ACE2 in tongue cells of postmenopausal compared with premenopausal (p = 0.05), and a strong negative correlation between saliva estrogen and viral load (r = -0.76; p = 0.001). Respect to IFN-γ (p = 0.05), IL-1β, TNF-α, IL-18, and IL-23 levels were increased in postmenopausal. Oral microbiome signature of premenopausal was characterized by Prevotella melaninogenica (Log2 = 26.68; p = 1.34e-10), Haemophilus (Log2 = 23.99; p = 2.96e-9), and Alloprevotella (Log2 = 7.92; p = 0.0001). On the other hand, Leptotrichia (Log2 = -18.74; p = 0.001), Tanerella (Log2 = -17.08; p = 0.004), and Clostridiales (Log2 = -2.88; p = 0.04) represented the poor oral health group compared with the adequate group which was enriched with the commensal microorganism Neisseria perflava (Log2 = 26.70; p = 1.74e-7). Furthermore, the high viral load group was characterized by Prevotella nanceiensis (Log2 = 19.60; p = 6.06e-8), Prevotella melaninogenica (Log2 = 21.45; p = 9.59e-6), Alloprevotella (Log2 = 23.50; p = 2.70e-7) and bacteria from the red complex Porphyromonas endodentalis (Log2 = 21.97; p = 1.38e-7). CONCLUSIONS: Postmenopausal and men have a poor oral health status which could be related to a detrimental progression of COVID-19 also linked to a lower expression of ACE2, lower saliva estrogen levels and oral dysbiosis. Nevertheless, functional studies are required for a deeper knowledge.

Experiment 1


Reviewed Marked as Reviewed by Svetlana up on 2024-6-21

Curated date: 2024/03/20

Curator: EniolaAde

Revision editor(s): EniolaAde, Victoria, Scholastica

Subjects

Location of subjects
Mexico
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Saliva Sailva normalis,Saliva atomaris,Saliva molecularis,Salivary gland secretion,Saliva,saliva
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Menopause Change of Life, Female,Menopause,menopause
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Premenopausal women group
Group 1 name Corresponds to the case (exposed) group for case-control studies
Postmenopausal women group
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Postmenopausal women with COVID-19
Group 0 sample size Number of subjects in the control (unexposed) group
20
Group 1 sample size Number of subjects in the case (exposed) group
18
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
30 days

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
raw counts
Statistical test
DESeq2
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No

Alpha Diversity

Chao1 Abundance-based estimator of species richness
unchanged

Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2024-6-21

Curated date: 2024/03/20

Curator: EniolaAde

Revision editor(s): EniolaAde, Scholastica

Source: Figure 3c

Description: DeSeq2 deferentially abundant taxa in premenopausal women compared to postmenopausal women group

Abundance in Group 1: increased abundance in Postmenopausal women group

NCBI Quality ControlLinks
Alloprevotella
Haemophilus
Hoylesella nanceiensis
Neisseria
Prevotella melaninogenica

Revision editor(s): EniolaAde, Scholastica

Signature 2

Reviewed Marked as Reviewed by Svetlana up on 2024-6-21

Curated date: 2024/03/26

Curator: EniolaAde

Revision editor(s): EniolaAde, Scholastica

Source: Figure 3c

Description: DeSeq2 deferentially abundant taxa in premenopausal women compared to postmenopausal women group

Abundance in Group 1: decreased abundance in Postmenopausal women group

NCBI Quality ControlLinks
Neisseria

Revision editor(s): EniolaAde, Scholastica

Experiment 2


Reviewed Marked as Reviewed by Svetlana up on 2024-6-21

Curated date: 2024/03/22

Curator: EniolaAde

Revision editor(s): EniolaAde, Victoria, Scholastica

Differences from previous experiment shown

Subjects

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Microbiome measurement Microbiome measurement,microbiome measurement
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Adequate oral health group
Group 1 name Corresponds to the case (exposed) group for case-control studies
Poor oral health group
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
COVID-19 patients with poor oral health status. Patients were classified as having poor oral health when there were more than two caries, hygiene index higher than 2.5, gingival inflammation between 2–3 and bleeding when toothbrushing.
Group 0 sample size Number of subjects in the control (unexposed) group
Not specified
Group 1 sample size Number of subjects in the case (exposed) group
Not specified

Lab analysis

Statistical Analysis

Alpha Diversity

Chao1 Abundance-based estimator of species richness
unchanged

Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2024-6-21

Curated date: 2024/03/22

Curator: EniolaAde

Revision editor(s): EniolaAde, Scholastica

Source: Figure 3f

Description: DESeq2 differentially abundant taxa in poor oral health group compared to adequate oral health group

Abundance in Group 1: increased abundance in Poor oral health group

NCBI Quality ControlLinks
Neisseria perflava
unclassified Neisseria
Clostridia UCG-014Clostridia UCG-014

Revision editor(s): EniolaAde, Scholastica

Signature 2

Reviewed Marked as Reviewed by Svetlana up on 2024-6-21

Curated date: 2024/03/22

Curator: EniolaAde

Revision editor(s): EniolaAde, Scholastica

Source: Figure 3f

Description: DESeq2 differentially abundant taxa in poor oral health group compared to adequate oral health group

Abundance in Group 1: decreased abundance in Poor oral health group

NCBI Quality ControlLinks
Alloprevotella
Bacteroidetes incertae sedis
Clostridiales bacterium
Prevotella melaninogenica
unclassified Alloprevotella
unclassified Leptotrichia
unclassified Neisseria
uncultured Bacteroidota bacterium
unclassified Haemophilus
unclassified Tannerella

Revision editor(s): EniolaAde, Scholastica

Experiment 3


Reviewed Marked as Reviewed by Svetlana up on 2024-6-21

Curated date: 2024/03/22

Curator: EniolaAde

Revision editor(s): EniolaAde, Victoria, Scholastica

Differences from previous experiment shown

Subjects

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Viral load viral burden,viral titer,viral titre,Viral load,viral load
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Moderate SARS-CoV-2 viral load group
Group 1 name Corresponds to the case (exposed) group for case-control studies
High SARS-CoV-2 viral load group
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
COVID-19 patients with high SARS-CoV-2 viral load
Group 0 sample size Number of subjects in the control (unexposed) group
11
Group 1 sample size Number of subjects in the case (exposed) group
41

Lab analysis

Statistical Analysis

Alpha Diversity

Chao1 Abundance-based estimator of species richness
unchanged

Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2024-6-21

Curated date: 2024/03/22

Curator: EniolaAde

Revision editor(s): EniolaAde, Scholastica

Source: Figure 3i

Description: DESeq2 differentially abundant taxa in moderate compared to high SARS-CoV-2 viral load group

Abundance in Group 1: increased abundance in High SARS-CoV-2 viral load group

NCBI Quality ControlLinks
Alloprevotella
Haemophilus
Hoylesella nanceiensis
Leptotrichia
Neisseria
Porphyromonas endodontalis
Prevotella melaninogenica
uncultured Bacteroidota bacterium
Pasteurellaceae_HaemophilusPasteurellaceae_Haemophilus

Revision editor(s): EniolaAde, Scholastica