Maternal microbiota communicates with the fetus through microbiota-derived extracellular vesicles/Experiment 1
From BugSigDB
Needs review
Subjects
- Location of subjects
- Finland
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Amniotic fluid Acqua amnii,Liquor amnii,Amniotic fluid,amniotic fluid
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Pregnancy Gestation,PREGN,Pregnancies,Pregnancy,pregnancy
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Amniotic fluid (AM)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Amniotic fluid-derived extracellular vesicles (AM EVs)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Amniotic fluid EV group
- Group 0 sample size Number of subjects in the control (unexposed) group
- 10
- Group 1 sample size Number of subjects in the case (exposed) group
- 24
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V4-V5
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Ion Torrent
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- raw counts
- Statistical test
- ANCOM
- Kruskall-Wallis
- Mann-Whitney (Wilcoxon)
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- increased
- Richness Number of species
- increased
Signature 1
Needs review
Source: Additional file 1: Table 8
Description: Differential abundance taxa of AM and AM_EV pair
Abundance in Group 1: increased abundance in Amniotic fluid-derived extracellular vesicles (AM EVs)
Revision editor(s): Iman-Ngwepe
