Effects of OsomeFood Clean Label plant-based meals on the gut microbiome

Study information

Reviewed Marked as Reviewed by Svetlana up on 2024-7-24

study design

time series / longitudinal observational

Citation

PMID PubMed identifier for scientific articles.

36997838

DOI Digital object identifier for electronic documents.

10.1186/s12866-023-02822-z

URI

https://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-023-02822-z

Authors

Jacky D, Bibi C, Meng LMC, Jason F, Gwendoline T, Jeremy L, Wie CC

Journal

BMC microbiology

Year

2023

Keywords:

Intervention, Metagenomic, Microbiota, Nutrition

BACKGROUND: Plant-based diets offer more beneficial microbes and can modulate gut microbiomes to improve human health. We evaluated the effects of the plant-based OsomeFood Clean Label meal range ('AWE' diet), on the human gut microbiome. METHODS: Over 21 days, ten healthy participants consumed OsomeFood meals for five consecutive weekday lunches and dinners and resumed their regular diets for other days/meals. On follow-up days, participants completed questionnaires to record satiety, energy and health, and provided stool samples. To document microbiome variations and identify associations, species and functional pathway annotations were analyzed by shotgun sequencing. Shannon diversity and regular diet calorie intake subsets were also assessed. RESULTS: Overweight participants gained more species and functional pathway diversity than normal BMI participants. Nineteen disease-associated species were suppressed in moderate-responders without gaining diversity, and in strong-responders with diversity gains along with health-associated species. All participants reported improved short-chain fatty acids production, insulin and γ-aminobutyric acid signaling. Moreover, fullness correlated positively with Bacteroides eggerthii; energetic status with B. uniformis, B. longum, Phascolarctobacterium succinatutens, and Eubacterium eligens; healthy status with Faecalibacterium prausnitzii, Prevotella CAG 5226, Roseburia hominis, and Roseburia sp. CAG 182; and overall response with E. eligens and Corprococcus eutactus. Fiber consumption was negatively associated with pathogenic species. CONCLUSION: Although the AWE diet was consumed for only five days a week, all participants, especially overweight ones, experienced improved fullness, health status, energy and overall responses. The AWE diet benefits all individuals, especially those of higher BMI or low-fiber consumption.

Experiment 1

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Reviewed Marked as Reviewed by Svetlana up on 2024-7-24

Curated date: 2024/03/22

Curator: ModinatG

Revision editor(s): ModinatG, Scholastica

Subjects

Location of subjects: Singapore

Host species Species from which microbiome was sampled. Contact us to have more species added.: Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Diet Dietary,Diets,Diet,diet

Group 0 name Corresponds to the control (unexposed) group for case-control studies: Participants before the AWE diet - D0

Group 1 name Corresponds to the case (exposed) group for case-control studies: Participants after the AWE diet - D21

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: Participants who strictly adhered to OsomeFoods plant-based meal plans for five consecutive days (Monday through to Friday; 'AWE') for 3 weeks.

Group 0 sample size Number of subjects in the control (unexposed) group: 10

Group 1 sample size Number of subjects in the case (exposed) group: 10

Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any): 3 months

Lab analysis

Sequencing type: WMS

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: Not specified

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).: relative abundances

Statistical test: Mann-Whitney (Wilcoxon)

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: Yes

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: unchanged

Signature 1

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Reviewed Marked as Reviewed by Svetlana up on 2024-7-24

Curated date: 2024/03/25

Curator: ModinatG

Revision editor(s): ModinatG

Source: Fig 2c

Description: Species differentially abundant between baseline and day 21 (End of the intervention period)

Abundance in Group 1: increased abundance in Participants after the AWE diet - D21

NCBI	Quality Control	Links
Bacteroides thetaiotaomicron
Bacteroides xylanisolvens
Leuconostoc garlicum

Revision editor(s): ModinatG

Signature 2

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Reviewed Marked as Reviewed by Svetlana up on 2024-7-24

Curated date: 2024/03/25

Curator: ModinatG

Revision editor(s): ModinatG

Source: Fig 2c

Description: Species differentially abundant between baseline and day 21 (End of the intervention period)

Abundance in Group 1: decreased abundance in Participants after the AWE diet - D21

NCBI	Quality Control	Links
Bacteroides phage B124-14
Collinsella intestinalis
Romboutsia ilealis
Weissella confusa

Revision editor(s): ModinatG