Stachyose ameliorates obesity-related metabolic syndrome via improving intestinal barrier function and remodeling gut microbiota/Experiment 1
From BugSigDB
Reviewed Marked as Reviewed by Svetlana up on 2024-6-9
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Mus musculus
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- High fat diet HF - High fat diet,High fat diet (finding),High fat diet,high fat diet
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- High-Fat Diet fed Mice (HFD)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Stachyose-treated High-Fat Diet fed Mice (STA)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Male C57BL/6J mice (18–20g, 6 weeks old) which were fed a High-Fat Diet (D12492-HFD diet, #XTHF60-1) with 60% of energy from fat, for 12 weeks and treatment with stachyose in the last 8 weeks (200 mg/ kg/day).
- Group 0 sample size Number of subjects in the control (unexposed) group
- 7
- Group 1 sample size Number of subjects in the case (exposed) group
- 7
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- None
Lab analysis
- Sequencing type
- WMS
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- Not specified
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- DNBSEQ-T7
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- LEfSe
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 2
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
Signature 1
Reviewed Marked as Reviewed by Svetlana up on 2024-6-9
Source: Figure 5A
Description: Bacterial taxa identified by Linear discriminant analysis effect size (LEfSe) as differentially abundant in mice fed with Stachyose-treated High-Fat Diet compared to mice fed with High-Fat Diet.
Abundance in Group 1: increased abundance in Stachyose-treated High-Fat Diet fed Mice (STA)
| NCBI | Quality Control | Links |
|---|---|---|
| Collinsella stercoris | ||
| Allobaculum mucilyticum | ||
| Thermophilibacter immobilis | ||
| Parolsenella catena | ||
| Parafannyhessea umbonata |
Revision editor(s): Joan Chuks
Signature 2
Reviewed Marked as Reviewed by Svetlana up on 2024-6-9
Source: Figure 5A
Description: Bacterial taxa identified by Linear discriminant analysis effect size (LEfSe) as differentially abundant in mice fed with Stachyose-treated High-Fat Diet compared to mice fed with High-Fat Diet.
Abundance in Group 1: decreased abundance in Stachyose-treated High-Fat Diet fed Mice (STA)
| NCBI | Quality Control | Links |
|---|---|---|
| Escherichia marmotae |
Revision editor(s): Joan Chuks
