Weight gain in anorexia nervosa does not ameliorate the faecal microbiota, branched chain fatty acid profiles, and gastrointestinal complaints/Experiment 2
From BugSigDB
Reviewed Marked as Reviewed by Lwaldron on 2022/06/24
Subjects
- Location of subjects
- Germany
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Anorexia nervosa anorexia nervosa,Anorexia nervosa
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- HC
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- anorexia patients after weight gain
- Group 0 sample size Number of subjects in the control (unexposed) group
- 55
- Group 1 sample size Number of subjects in the case (exposed) group
- 44
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- 8 weeks
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- Mann-Whitney (Wilcoxon)
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.15
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
- Confounders controlled for Confounding factors that have been accounted for by stratification or model adjustment
- age, sex
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- increased
- Chao1 Abundance-based estimator of species richness
- unchanged
Signature 1
Reviewed Marked as Reviewed by Lwaldron on 2022/06/24
Source: figure 3
Description: gut microbiota of anorexia patients before and after weight gain compared to healthy controls
Abundance in Group 1: increased abundance in anorexia patients after weight gain
| NCBI | Quality Control | Links |
|---|---|---|
| Actinomycetota | ||
| Anaerostipes | ||
| Bifidobacterium | ||
| Bacillota | ||
| Lactobacillus | ||
| Lactococcus | ||
| Pseudoflavonifractor | ||
| Ruminococcus | ||
| Sporobacter | ||
| unclassified Clostridium |
Signature 2
Reviewed Marked as Reviewed by Lwaldron on 2022/06/24
Source: figure 3
Description: gut microbiota of anorexia patients before and after weight gain compared to healthy controls
Abundance in Group 1: decreased abundance in anorexia patients after weight gain
| NCBI | Quality Control | Links |
|---|---|---|
| Bacteroides | ||
| Bacteroidota | ||
| unclassified Clostridium |
