Analysis of the cervical microbiome and potential biomarkers from postpartum HIV-positive women displaying cervical intraepithelial lesions/Experiment 1

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Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks

Subjects

Location of subjects

Brazil

Host species Species from which microbiome was sampled. Contact us to have more species added.

Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology

Uterus Uterus,uterus

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology

Cervical glandular intraepithelial neoplasia Cervical glandular intraepithelial neoplasia,cervical glandular intraepithelial neoplasia

Group 0 name Corresponds to the control (unexposed) group for case-control studies

HIV pateints with normal tissue at 6 months

Group 1 name Corresponds to the case (exposed) group for case-control studies

HIV patients with lesion at 12 months

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group

Cases were identified by HIV rapid or ELISA test and subsequent Western blot following recommendations for HIV diagnosis by the Brazilian Ministry of Health.

Group 0 sample size Number of subjects in the control (unexposed) group

5

Group 1 sample size Number of subjects in the case (exposed) group

5

Lab analysis

Sequencing type

16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene

V3-V6

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance

Illumina

Statistical Analysis

Statistical test

Mann-Whitney (Wilcoxon)

Significance threshold p-value or FDR threshold used for differential abundance testing (if any)

0.1

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?

No

Signature 1

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Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

Curated date: 2021/01/10

Curator: Shaimaa Elsafoury

Revision editor(s): WikiWorks

Source: Figure 4

Description: Gardnerella vaginalis abundance longitudinal analysis at six and twelve months postpartum in paired samples

Abundance in Group 1: decreased abundance in HIV patients with lesion at 12 months

NCBI	Quality Control	Links
Gardnerella vaginalis

Revision editor(s): WikiWorks