The intensive physical activity causes changes in the composition of gut and oral microbiota
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Quality control
- Retracted paper
- Contamination issues suspected
- Batch effect issues suspected
- Uncontrolled confounding suspected
- Results are suspect (various reasons)
- Tags applied
Experiment 1
Subjects
- Location of subjects
- Poland
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Mouth Adult mouth,Cavital oralis,Cavitas oris,Cavum oris,Mouth cavity,Oral region,Oral vestibule,Regio oralis,Rima oris,Stoma,Stomatodaeum,Trophic apparatus,Vestibule of mouth,Vestibulum oris,Mouth,mouth
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Physical activity Activities, Locomotor,Activities, Motor,Activities, Physical,Activity, Locomotor,Activity, Motor,Activity, Physical,Locomotor Activities,Locomotor Activity,Motor Activities,Motor Activity,Physical Activities,Physical activity,physical activity
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Amateurs
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Professional football players
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Participants in group 1 are professional football players who had oral swabs taken.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 12
- Group 1 sample size Number of subjects in the case (exposed) group
- 20
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- 2 months
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- LEfSe
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 2
- Matched on Factors on which subjects have been matched on in a case-control study
- age, body mass index, health
Alpha Diversity
- Chao1 Abundance-based estimator of species richness
- unchanged
- Richness Number of species
- unchanged
Signature 1
Source: Figure 9a
Description: Linear discriminant analysis (LDA) scores of differentially abundant species among amateurs and professional football players.
Abundance in Group 1: increased abundance in Professional football players
Revision editor(s): Aleru Divine
Signature 2
Source: Figure 9a
Description: Linear discriminant analysis (LDA) scores of differentially abundant species among amateurs and professional football players.
Abundance in Group 1: decreased abundance in Professional football players
| NCBI | Quality Control | Links |
|---|---|---|
| Prevotella histicola | ||
| Prevotella melaninogenica | ||
| Actinomyces graevenitzii | ||
| Haemophilus sputorum | ||
| Schaalia odontolytica |
Revision editor(s): Aleru Divine
Experiment 2
Subjects
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Participants in group 1 are professional football players who had stool samples taken.
Lab analysis
Statistical Analysis
Alpha Diversity
- Chao1 Abundance-based estimator of species richness
- increased
- Richness Number of species
- increased
Signature 1
Source: Figure 9b
Description: Linear discriminant analysis (LDA) scores of differentially abundant species among amateurs and professional football players
Abundance in Group 1: increased abundance in Professional football players
Revision editor(s): Aleru Divine
Signature 2
Source: Figure 9b
Description: Linear discriminant analysis (LDA) scores of differentially abundant species among amateurs and professional football players.
Abundance in Group 1: decreased abundance in Professional football players
| NCBI | Quality Control | Links |
|---|---|---|
| Bifidobacterium adolescentis | ||
| Bifidobacterium longum | ||
| Dialister succinatiphilus | ||
| Lachnospiraceae bacterium | ||
| Phocaeicola coprocola | ||
| Phocaeicola coprophilus | ||
| Roseburia inulinivorans |
Revision editor(s): Aleru Divine
Experiment 3
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Professional football players before intensive training
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Professional football players after intensive training
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Participants in group 1 are professional football players who had stool samples taken after intensive training.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 20
Lab analysis
Statistical Analysis
Alpha Diversity
- Chao1 Abundance-based estimator of species richness
- increased
- Richness Number of species
- increased
Signature 1
Source: Figure 10
Description: LEfSe algorithm showing the features with significant differential abundance in the gut microbiota of professional football players before intensive training and after intensive training.
Abundance in Group 1: increased abundance in Professional football players after intensive training
| NCBI | Quality Control | Links |
|---|---|---|
| Lactobacillales | ||
| Streptococcaceae | ||
| Streptococcus |
Revision editor(s): Aleru Divine
Signature 2
Source: Figure 10
Description: LEfSe algorithm showing the features with significant differential abundance in the gut microbiota of professional football players before intensive training and after intensive training.
Abundance in Group 1: decreased abundance in Professional football players after intensive training
| NCBI | Quality Control | Links |
|---|---|---|
| Barnesiella | ||
| Barnesiellaceae |
Revision editor(s): Aleru Divine
Experiment 4
Subjects
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Mouth Adult mouth,Cavital oralis,Cavitas oris,Cavum oris,Mouth cavity,Oral region,Oral vestibule,Regio oralis,Rima oris,Stoma,Stomatodaeum,Trophic apparatus,Vestibule of mouth,Vestibulum oris,Mouth,mouth
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Participants in group 1 are professional football players who had oral swabs taken after intensive training.
Lab analysis
Statistical Analysis
Alpha Diversity
- Chao1 Abundance-based estimator of species richness
- decreased
- Richness Number of species
- decreased
Signature 1
Source: Figure 11
Description: LEfSe algorithm showing the features with significant differential abundance in the oral microbiota of professional football players before intensive training and after intensive training.
Abundance in Group 1: increased abundance in Professional football players after intensive training
Revision editor(s): Aleru Divine
Signature 2
Source: Figure 11
Description: LEfSe algorithm showing the features with significant differential abundance in the oral microbiota of professional football players before intensive training and after intensive training.
Abundance in Group 1: decreased abundance in Professional football players after intensive training
| NCBI | Quality Control | Links |
|---|---|---|
| Bacteroidales | ||
| Carnobacteriaceae | ||
| Eubacteriales | ||
| Granulicatella | ||
| Lachnospiraceae | ||
| Neisseria perflava | ||
| Neisseriaceae | ||
| Neisseriales | ||
| Oribacterium | ||
| Prevotella | ||
| Prevotellaceae |
Revision editor(s): Aleru Divine
