The intensive physical activity causes changes in the composition of gut and oral microbiota/Experiment 2

From BugSigDB


Reviewed Marked as Reviewed by ChiomaBlessing on 2024-10-15

Curated date: 2024/10/02

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Subjects

Location of subjects
Poland
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Physical activity Activities, Locomotor,Activities, Motor,Activities, Physical,Activity, Locomotor,Activity, Motor,Activity, Physical,Locomotor Activities,Locomotor Activity,Motor Activities,Motor Activity,Physical Activities,Physical activity,physical activity
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Amateurs
Group 1 name Corresponds to the case (exposed) group for case-control studies
Professional football players
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Participants in group 1 are professional football players who had stool samples taken.
Group 0 sample size Number of subjects in the control (unexposed) group
12
Group 1 sample size Number of subjects in the case (exposed) group
20
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
2 months

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
LEfSe
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
2
Matched on Factors on which subjects have been matched on in a case-control study
age, body mass index, health

Alpha Diversity

Chao1 Abundance-based estimator of species richness
increased
Richness Number of species
increased

Signature 1

Reviewed Marked as Reviewed by ChiomaBlessing on 2024-10-15

Curated date: 2024/10/02

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Source: Figure 9b

Description: Linear discriminant analysis (LDA) scores of differentially abundant species among amateurs and professional football players

Abundance in Group 1: increased abundance in Professional football players

NCBI Quality ControlLinks
Blautia wexlerae
Holdemanella biformis
Mediterraneibacter faecis
Neglectibacter timonensis
Parabacteroides johnsonii
Phascolarctobacterium faecium
Streptococcus salivarius
[Ruminococcus] lactaris

Revision editor(s): Aleru Divine

Signature 2

Reviewed Marked as Reviewed by ChiomaBlessing on 2024-10-15

Curated date: 2024/10/02

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Source: Figure 9b

Description: Linear discriminant analysis (LDA) scores of differentially abundant species among amateurs and professional football players.

Abundance in Group 1: decreased abundance in Professional football players

NCBI Quality ControlLinks
Bifidobacterium adolescentis
Bifidobacterium longum
Dialister succinatiphilus
Lachnospiraceae bacterium
Phocaeicola coprocola
Phocaeicola coprophilus
Roseburia inulinivorans

Revision editor(s): Aleru Divine