Preconception helminth infection alters offspring microbiota and immune subsets in a mouse model/Experiment 3
From BugSigDB
Reviewed Marked as Reviewed by Svetlana up on 2024-11-5
Subjects
- Location of subjects
- South Africa
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Mus musculus
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Helminthiasis, animal animal Helminthiases,animal helminthiasis,Helminthiases, animal,Helminthiasis, animal,helminthiasis, animal
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- NvM pups
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- NbM pups
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- NbM pups are the babies given birth to by the NbM mothers.
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V6
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- log transformation
- Statistical test
- DESeq2
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- increased
Signature 1
Reviewed Marked as Reviewed by Svetlana up on 2024-11-5
Source: Figure 3D
Description: Differential abundant bacteria at different taxonomy level
Abundance in Group 1: increased abundance in NbM pups
| NCBI | Quality Control | Links |
|---|---|---|
| Erysipelotrichaceae | ||
| Coriobacteriaceae | ||
| Micrococcaceae | ||
| Comamonadaceae |
Revision editor(s): KateRasheed
Signature 2
Reviewed Marked as Reviewed by Svetlana up on 2024-11-5
Source: Figure 3D
Description: Differential abundant bacteria at different taxonomy level
Abundance in Group 1: decreased abundance in NbM pups
| NCBI | Quality Control | Links |
|---|---|---|
| Clostridiaceae | ||
| Enterobacteriaceae | ||
| Corynebacteriaceae | ||
| Carnobacteriaceae |
Revision editor(s): KateRasheed
