Preconception helminth infection alters offspring microbiota and immune subsets in a mouse model/Experiment 4

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Reviewed Marked as Reviewed by Svetlana up on 2024-11-5

Curated date: 2024/10/21

Curator: Prolific

Revision editor(s): Prolific, KateRasheed, Victoria

Subjects

Location of subjects

South Africa

Host species Species from which microbiome was sampled. Contact us to have more species added.

Mus musculus

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology

Spleen Lien,Spleen,spleen

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology

Helminthiasis, animal animal Helminthiases,animal helminthiasis,Helminthiases, animal,Helminthiasis, animal,helminthiasis, animal

Group 0 name Corresponds to the control (unexposed) group for case-control studies

Low CD4+CD44hi Group

Group 1 name Corresponds to the case (exposed) group for case-control studies

High CD4+CD44hi Group

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group

High refers to the CD4 + CD44hi frequency around the mean of samples from NbM pups.

Group 0 sample size Number of subjects in the control (unexposed) group

7

Group 1 sample size Number of subjects in the case (exposed) group

6

Lab analysis

Sequencing type

16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene

V6

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance

Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).

log transformation

Statistical test

DESeq2

Significance threshold p-value or FDR threshold used for differential abundance testing (if any)

0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?

Yes

Signature 1

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Reviewed Marked as Reviewed by Svetlana up on 2024-11-5

Curated date: 2024/10/22

Curator: Prolific

Revision editor(s): Prolific, KateRasheed

Source: Figure 5H

Description: Differential abundance of microbiota in the CD4 + CD44hi frequency around the mean of samples from NbM pups.

Abundance in Group 1: increased abundance in High CD4+CD44hi Group

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Coprobacter
Gordonibacter
Clostridium IVClostridium IV

Revision editor(s): Prolific, KateRasheed

Signature 2

EditHistoryDelete

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Your review change was submitted

Reviewed Marked as Reviewed by Svetlana up on 2024-11-5

Curated date: 2024/10/22

Curator: Prolific

Revision editor(s): Prolific, KateRasheed

Source: Figure 5H

Description: Differential abundance of microbiota in the CD4 + CD44hi frequency around the mean of samples from NbM pups.

Abundance in Group 1: decreased abundance in High CD4+CD44hi Group

NCBI	Quality Control	Links
Aerococcus
Mobilitalea

Revision editor(s): Prolific, KateRasheed