Dysbiosis of Gut Microbiota and Short-Chain Fatty Acids in Acute Ischemic Stroke and the Subsequent Risk for Poor Functional Outcomes
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Study information
-
Quality control
- Retracted paper
- Contamination issues suspected
- Batch effect issues suspected
- Uncontrolled confounding suspected
- Results are suspect (various reasons)
- Tags applied
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
Tan C, Wu Q, Wang H, Gao X, Xu R, Cui Z, Zhu J, Zeng X, Zhou H, He Y, Yin J
Journal
JPEN. Journal of parenteral and enteral nutrition
Year
2021
Keywords:
dysbiosis, functional outcome, gut microbiota, ischemic stroke, short-chain fatty acids
BACKGROUND: The intestinal microbiota and its metabolites have been reported to play an important role in stroke. Gut microbiota-originating short-chain fatty acids (SCFAs) modulate brain functions directly or indirectly through immune, endocrine, vagal, and other humoral pathways. However, relatively few investigations have evaluated the gut microbiome and SCFAs spectrum or their potential associations with stroke outcomes in acute ischemic stroke (AIS) patients with different stroke severities. METHODS: We used 16S rRNA gene sequencing and gas chromatography to compare the fecal microbial composition and SCFA spectrum between AIS patients (n = 140) and healthy controls (n = 92). Their associations with 90-day poor functional outcomes were evaluated by logistic regression models. RESULTS: We found that the intestinal microbiota distinguished AIS patients from healthy controls. A lack of SCFAs-producing bacteria and a low fecal SCFAs level defined dysbiosis in AIS patients, especially those with increased stroke severity. The SCFAs levels were negatively correlated with stroke severity and prognosis. Reduced SCFAs levels, especially acetate, were associated with an increased risk of 90-day poor functional outcomes even after adjustments. CONCLUSIONS: Dysbiosis of SCFAs-producing bacteria and SCFAs in AIS patients increased the subsequent risk for poor functional outcomes, indicating that SCFAs could be potential prognostic markers and therapeutic targets for stroke.
Experiment 1
Reviewed Marked as Reviewed by Folakunmi on 2024-11-7
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Ischemic stroke Ischaemic stroke,Ischemic stroke,ischemic stroke
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Healthy control group (HC)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Acute ischemic stroke (AIS)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- All acute ischemic stroke patients with ischemic stroke onset within 48 hours.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 92
- Group 1 sample size Number of subjects in the case (exposed) group
- 140
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- No antibiotics, prebiotics or probiotics taken in the past 3 months.
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- LEfSe
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 2.5
Signature 1
Reviewed Marked as Reviewed by Folakunmi on 2024-11-7
Source: Figure 2D
Description: The difference between the gut microbiota of patients with acute ischemic stroke and healthy controls by LEfSE
Abundance in Group 1: decreased abundance in Acute ischemic stroke (AIS)
| NCBI | Quality Control | Links |
|---|---|---|
| Alcaligenaceae | ||
| Bacteroidaceae | ||
| Lachnospiraceae | ||
| Moraxellaceae | ||
| Pasteurellaceae | ||
| Prevotellaceae |
Experiment 2
Reviewed Marked as Reviewed by Folakunmi on 2024-11-13
Differences from previous experiment shown
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Healthy control/moderate AIS/ severe AIS
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Mild acute ischemic stroke
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Ischemic stroke Patients with a NIHSS score < 5 points
- Group 0 sample size Number of subjects in the control (unexposed) group
- 154
- Group 1 sample size Number of subjects in the case (exposed) group
- 78
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- No antibiotic, probiotics or prebiotic taken in the past 3 months.
Lab analysis
Statistical Analysis
Signature 1
Reviewed Marked as Reviewed by Folakunmi on 2024-11-13
Source: figure 2D
Description: These taxons show an increased abundance in mild acute ischemic patients as compared to the moderate/severe AIS patients and healthy controls by LEfSE
Abundance in Group 1: increased abundance in Mild acute ischemic stroke
| NCBI | Quality Control | Links |
|---|---|---|
| Deferribacteraceae | ||
| Helicobacteraceae | ||
| Lactobacillaceae | ||
| Micrococcaceae | ||
| Nitrobacteraceae |
Experiment 3
Reviewed Marked as Reviewed by Folakunmi on 2024-11-7
Differences from previous experiment shown
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Healthy control/mild AIS/ severe AIS
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Moderate acute ischemic stroke
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Ischemic stroke Patients with a NIHSS score between 5 and 15 points
- Group 0 sample size Number of subjects in the control (unexposed) group
- 185
- Group 1 sample size Number of subjects in the case (exposed) group
- 47
Lab analysis
Statistical Analysis
Signature 1
Reviewed Marked as Reviewed by Folakunmi on 2024-11-7
Source: Figure 2D
Description: These taxons are increased in moderate AIS as compared to healthy control/mild AIS/severe AIS
Abundance in Group 1: increased abundance in Moderate acute ischemic stroke
| NCBI | Quality Control | Links |
|---|---|---|
| Enterobacteriaceae | ||
| Enterococcaceae | ||
| Erysipelotrichaceae | ||
| Methanobacteriaceae | ||
| Corynebacteriaceae | ||
| Eubacteriaceae |
Revision editor(s): InimfonD
Experiment 4
Reviewed Marked as Reviewed by Folakunmi on 2024-11-7
Differences from previous experiment shown
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Healthy control/mild AIS/ moderate AIS
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Severe acute ischemic stroke
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Ischemic stroke Patients with a NIHSS score >15 points
- Group 0 sample size Number of subjects in the control (unexposed) group
- 217
- Group 1 sample size Number of subjects in the case (exposed) group
- 15
Lab analysis
Statistical Analysis
Signature 1
Reviewed Marked as Reviewed by Folakunmi on 2024-11-7
Source: figure 2D
Description: These taxa show increase in severe AIS compared to healthy control/mild AIS/moderate AIS by LEfSE
Abundance in Group 1: increased abundance in Severe acute ischemic stroke
| NCBI | Quality Control | Links |
|---|---|---|
| Porphyromonadaceae | ||
| Mycoplasmataceae | ||
| Phyllobacteriaceae | ||
| Carnobacteriaceae | ||
| Staphylococcaceae |
Revision editor(s): InimfonD
Experiment 5
Reviewed Marked as Reviewed by Folakunmi on 2024-11-13
Differences from previous experiment shown
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Healthy control group (HC)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Acute ischemic stroke (AIS)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- All acute ischemic stroke patients with ischemic stroke onset within 48 hours.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 92
- Group 1 sample size Number of subjects in the case (exposed) group
- 140
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- No antibiotics, prebiotics or probiotics taken in the past 3 months.
Lab analysis
Statistical Analysis
Signature 1
Reviewed Marked as Reviewed by Folakunmi on 2024-11-13
Source: Figure 3
Description: Comparison of the abundance of short-chain fatty acid-producing gut microbiota between healthy controls and Acute Ischemic Stroke patients according to taxonomic classification and LEfSe analysis.
Abundance in Group 1: increased abundance in Acute ischemic stroke (AIS)
| NCBI | Quality Control | Links |
|---|---|---|
| Akkermansia | ||
| Enterobacteriaceae | ||
| Lactobacillaceae | ||
| Porphyromonadaceae |
Signature 2
Reviewed Marked as Reviewed by Folakunmi on 2024-11-13
Source: Figure 3
Description: Comparison of the abundance of short-chain fatty acid-producing microbiota in healthy controls versus acute ischemic stroke patients according to taxonomic classification and LEfSe analysis.
Abundance in Group 1: decreased abundance in Acute ischemic stroke (AIS)
| NCBI | Quality Control | Links |
|---|---|---|
| Anaerostipes | ||
| Bacteroides | ||
| Blautia | ||
| Faecalibacterium | ||
| Lachnospiraceae | ||
| Roseburia |
Experiment 6
Reviewed Marked as Reviewed by Folakunmi on 2024-11-13
Differences from previous experiment shown
Subjects
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Stroke outcome severity measurement Stroke outcome severity measurement,stroke outcome severity measurement
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Moderate Acute ischemic stroke (AIS)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Mild (AIS)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Acute ischemic stroke Patients with National Institutes of Health Stroke Scale (NIHSS) score < 5 points
- Group 0 sample size Number of subjects in the control (unexposed) group
- 47
- Group 1 sample size Number of subjects in the case (exposed) group
- 78
Lab analysis
Statistical Analysis
Signature 1
Reviewed Marked as Reviewed by Folakunmi on 2024-11-13
Source: Figure 3
Description: Comparison of the abundance of short-chain fatty acid-producing gut microbiota between moderate and mild acute ischemic patients according to taxonomic classification and LEfSe analysis.
Abundance in Group 1: increased abundance in Mild (AIS)
| NCBI | Quality Control | Links |
|---|---|---|
| Roseburia |
Signature 2
Reviewed Marked as Reviewed by Folakunmi on 2024-11-13
Source: Figure 3
Description: Comparison of the abundance of short-chain fatty acid-producing gut microbiota between moderate and mild acute ischemic patients according to taxonomic classification and LEfSe analysis.
Abundance in Group 1: decreased abundance in Mild (AIS)
| NCBI | Quality Control | Links |
|---|---|---|
| Akkermansia |
Experiment 7
Reviewed Marked as Reviewed by Folakunmi on 2024-11-13
Differences from previous experiment shown
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Mild Acute ischemic stroke (AIS)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Severe Acute Ischemic Stroke (AIS)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Acute ischemic stroke Patients with National Institutes of Health Stroke Scale (NIHSS) score > 15 points
- Group 0 sample size Number of subjects in the control (unexposed) group
- 78
- Group 1 sample size Number of subjects in the case (exposed) group
- 15
Lab analysis
Statistical Analysis
Signature 1
Reviewed Marked as Reviewed by Folakunmi on 2024-11-13
Source: Figure 3
Description: Comparison of the abundance of short-chain fatty acid-producing gut microbiota in severe acute ischemic stroke patients versus mild ischemic stroke patients according to taxonomic classification and LEfSe analysis”
Abundance in Group 1: decreased abundance in Severe Acute Ischemic Stroke (AIS)
| NCBI | Quality Control | Links |
|---|---|---|
| Roseburia |
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