Gut Microbiome Features of Chinese Patients Newly Diagnosed with Alzheimer's Disease or Mild Cognitive Impairment

From BugSigDB
Needs review
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
Guo M, Peng J, Huang X, Xiao L, Huang F, Zuo Z
Journal
Journal of Alzheimer's disease : JAD
Year
2021
Keywords:
Alzheimer’s disease, Chinese, gut microbiome, mild cognitive impairment
BACKGROUND: Patients with Alzheimer's disease (AD) have gut microbiome alterations compared with healthy controls. However, previous studies often assess AD patients who have been on medications or other interventions for the disease. Also, simultaneous determination of gut microbiome in patients with mild cognitive impairment (MCI) or AD in a study is rare. OBJECTIVE: To determine whether there was a gut microbiome alteration in patients newly diagnosed with AD or MCI and whether the degree of gut microbiome alteration was more severe in patients with AD than patients with MCI. METHODS: Fecal samples of 18 patients with AD, 20 patients with MCI, and 18 age-matched healthy controls were collected in the morning for 16S ribosomal RNA sequencing. No patient had medications or interventions for AD or MCI before the samples were collected. RESULTS: Although there was no difference in the microbial α-diversity among the three groups, patients with AD or MCI had increased β-diversity compared with healthy controls. Patients with AD had decreased Bacteroides, Lachnospira, and Ruminiclostridium_9 and increased Prevotella at the genus level compared with healthy controls. The changing direction of these genera in patients with MCI was the same as patients with AD. However, Lachnospira was the only genus whose abundance in patients with MCI was statistically significantly lower than healthy controls. Bacteroides, Lachnospira, and Ruminiclostridium_9 were positively associated with better cognitive functions whereas Prevotella was on the contrary when subjects of all three groups were considered. The negative correlation of Prevotella with cognitive functions remained among patients with MCI. CONCLUSION: Patients newly diagnosed with AD or MCI have gut dysbiosis that includes the decrease of potentially protective microbiome, such as Bacteroides, and the increase of microbiome that can promote inflammation, such as Prevotella. Our results support a novel idea that the degree of gut dysbiosis is worsened with the disease stage from MCI to AD.

Experiment 1


Needs review

Curated date: 2024/10/27

Curator: AaishahM

Revision editor(s): AaishahM

Subjects

Location of subjects
China
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Alzheimer's disease [X]Dementia in Alzheimer's disease,[X]Dementia in Alzheimer's disease (disorder),AD,AD - Alzheimer's disease,Alzheimer Dementia,Alzheimer dementia,Alzheimer Dementia, Presenile,ALZHEIMER DIS,Alzheimer Disease,Alzheimer disease,Alzheimer disease, familial,Alzheimer Type Dementia,Alzheimer's,Alzheimer's Dementia,Alzheimer's dementia,Alzheimer's disease,Alzheimer's disease (disorder),Alzheimer's disease, NOS,Alzheimers,Alzheimers Dementia,Alzheimers dementia,ALZHEIMERS DIS,Alzheimers disease,DAT - Dementia Alzheimer's type,Dementia in Alzheimer's disease,Dementia in Alzheimer's disease (disorder),Dementia in Alzheimer's disease, unspecified (disorder),Dementia of the Alzheimer's type,Dementia, Alzheimer Type,Dementia, Presenile,Dementia, Presenile Alzheimer,Disease, Alzheimer,Disease, Alzheimer's,Presenile Alzheimer Dementia,sporadic Alzheimer's disease,alzheimer's disease
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Healthy controls (CON)
Group 1 name Corresponds to the case (exposed) group for case-control studies
Alzheimer's Disease (AD)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Patients newly diagnosed with Alzheimer's Disease
Group 0 sample size Number of subjects in the control (unexposed) group
18
Group 1 sample size Number of subjects in the case (exposed) group
18

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
LEfSe
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.01
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
2.0
Matched on Factors on which subjects have been matched on in a case-control study
age, Matched on: "gender" is not in the list (abnormal glucose tolerance, acetaldehyde, acute graft vs. host disease, acute lymphoblastic leukemia, acute myeloid leukemia, adenoma, age, AIDS, alcohol consumption measurement, alcohol drinking, ...) of allowed values.gender


Signature 1

Needs review

Curated date: 2024/11/03

Curator: AaishahM

Revision editor(s): AaishahM

Source: Figure 3 A + Figure 4

Description: Different abundances in bacterial taxa among AD and control patients

Abundance in Group 1: increased abundance in Alzheimer's Disease (AD)

NCBI Quality ControlLinks
Aeromonadales
Paraprevotella
Prevotellaceae
Succinivibrionaceae
uncultured bacterium
s_Prevotella_9_Others_Prevotella_9_Other
g_Prevotella_9g_Prevotella_9

Revision editor(s): AaishahM

Signature 2

Needs review

Curated date: 2024/11/03

Curator: AaishahM

Revision editor(s): AaishahM

Source: Figure 3 A

Description: Different abundances in bacterial taxa among AD and control patients

Abundance in Group 1: decreased abundance in Alzheimer's Disease (AD)

NCBI Quality ControlLinks
Acidaminococcaceae
Acidaminococcales
Bacteroidaceae
Bacteroides
Bacteroides fragilis
Blautia
Burkholderiaceae
Cloacibacillus
Dorea
Flavobacteriaceae
Flavobacteriales
Gammaproteobacteria
Lachnospira
Lachnospiraceae
Parabacteroides
Phascolarctobacterium
Pseudomonadota
Synergistaceae
Synergistales
Synergistia
Synergistota
Tannerellaceae
g_Ruminiclostridium_9g_Ruminiclostridium_9
s_Blautia_Others_Blautia_Other
s_Parabacteroides_Others_Parabacteroides_Other
o_Betaproteobacterialeso_Betaproteobacteriales
s_Cloacibacillus_Others_Cloacibacillus_Other
s_Lachnospira_Others_Lachnospira_Other
s_Phascolarctobacterium_Others_Phascolarctobacterium_Other
g_Flavobacteriaceae_Otherg_Flavobacteriaceae_Other

Revision editor(s): AaishahM

Experiment 2


Needs review

Curated date: 2024/11/04

Curator: AaishahM

Revision editor(s): AaishahM

Differences from previous experiment shown

Subjects

Group 1 name Corresponds to the case (exposed) group for case-control studies
Mild Cognitive Impairment (MCI)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Patients with mild cognitive impairment
Group 1 sample size Number of subjects in the case (exposed) group
20

Lab analysis

Statistical Analysis

Signature 1

Needs review

Curated date: 2024/11/04

Curator: AaishahM

Revision editor(s): AaishahM

Source: Figure 3 B

Description: Different abundances in bacterial taxa among MCI and control patients

Abundance in Group 1: increased abundance in Mild Cognitive Impairment (MCI)

NCBI Quality ControlLinks
Prevotella
Mycoplasmatota
Mollicutes
Negativibacillus
uncultured Alistipes sp.

Revision editor(s): AaishahM

Signature 2

Needs review

Curated date: 2024/11/04

Curator: AaishahM

Revision editor(s): AaishahM

Source: Figure 3 B

Description: Different abundances in bacterial taxa among MCI and control patients

Abundance in Group 1: decreased abundance in Mild Cognitive Impairment (MCI)

NCBI Quality ControlLinks
Blautia massiliensis (ex Durand et al. 2017)
Dorea
Veillonella
Bacteroides fragilis
Lachnospira
Bacteroidaceae
Bacteroides

Revision editor(s): AaishahM

Experiment 3


Needs review

Curated date: 2024/11/14

Curator: AaishahM

Revision editor(s): AaishahM

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
Mild Cognitive Impairment (MCI)
Group 1 name Corresponds to the case (exposed) group for case-control studies
Alzheimer's Disease (AD)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Patients newly diagnosed with Alzheimer's Disease
Group 0 sample size Number of subjects in the control (unexposed) group
20
Group 1 sample size Number of subjects in the case (exposed) group
18
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
6 months

Lab analysis

Statistical Analysis

Signature 1

Needs review

Curated date: 2024/11/14

Curator: AaishahM

Revision editor(s): AaishahM

Source: Figure 3 C

Description: Different abundances in bacterial taxa among AD and MCI patients

Abundance in Group 1: decreased abundance in Alzheimer's Disease (AD)

NCBI Quality ControlLinks
Pyramidobacter piscolens
Pyramidobacter
Flavobacteriaceae
Victivallales
Lentisphaerota
Lentisphaeria
Flavobacteriales
Synergistota
Merdimmobilis hominis
Synergistales
Synergistia
Cloacibacillus
Synergistaceae
Merdimmobilis
uncultured Roseburia sp.
Negativibacillus
Mollicutes
Mycoplasmatota
Oscillibacter
Sutterella
Burkholderiaceae
Pseudomonadota

Revision editor(s): AaishahM