Inactivation of the MSTN gene expression changes the composition and function of the gut microbiome in sheep

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Reviewed Marked as Reviewed by Svetlana up on 2024-11-27
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
Du C, Zhou X, Zhang K, Huang S, Wang X, Zhou S, Chen Y
Journal
BMC microbiology
Year
2022
Keywords:
Gut microbiome, Microbiota function, Myostatin edited, Sheep
BACKGROUND: Myostatin (MSTN) negatively regulates the muscle growth in animals and MSTN deficient sheep have been widely reported previously. The goal of this study was to explore how MSTN inactivation influences their gut microbiota composition and potential functions. RESULTS: We compared the slaughter parameters and meat quality of 3 MSTN-edited male sheep and 3 wild-type male sheep, and analyzed the gut microbiome of the MSTN-edited sheep (8 female and 8 male sheep) and wild-type sheep (8 female and 8 male sheep) through metagenomic sequencing. The results showed that the body weight, carcass weight and eye muscle area of MSTN-edited sheep were significantly higher, but there were no significant differences in the meat quality indexes. At the microbial level, the alpha diversity was significantly higher in the MSTN-edited sheep (P < 0.05), and the microbial composition was significantly different by PCoA analysis in the MSTN-edited and wild-type sheep. The abundance of Firmicutes significantly increased and Bacteroidota significantly decreased in the MSTN-edited sheep. At genus level, the abundance of Flavonifractor, Subdoligranulum, Ruthenibacterium, Agathobaculum, Anaerotignum, Oribacterium and Lactobacillus were significantly increased in the MSTN-edited sheep (P < 0.05). Further analysis of functional differences was found that the carotenoid biosynthesis was significantly increased and the peroxisome, apoptosis, ferroptosis, N-glycan biosynthesis, thermogenesis, and adipocytokines pathways were decreased in the MSTN-edited sheep (P < 0.05). Moreover, carbohydrate-active enzymes (CAZymes) results certified the abundance of the GH13_39, GH4, GH137, GH71 and PL17 were upregulated, and the GT41 and CBM20 were downregulated in the MSTN-edited sheep (P < 0.05). CONCLUSIONS: Our study suggested that MSTN inactivation remarkably influenced the composition and potential function of hindgut microbial communities of the sheep, and significantly promoted growth performance without affecting meat quality.

Experiment 1


Reviewed Marked as Reviewed by Svetlana up on 2024-11-27

Curated date: 2024/10/22

Curator: KateRasheed

Revision editor(s): KateRasheed

Subjects

Location of subjects
China
Host species Species from which microbiome was sampled. Contact us to have more species added.
Ovis aries
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Myostatin-related muscle hypertrophy MSLHP,muscle hypertrophy,myostatin-related muscle hypertrophy,Myostatin-related muscle hypertrophy
Group 0 name Corresponds to the control (unexposed) group for case-control studies
WTF(Wild-type female)
Group 1 name Corresponds to the case (exposed) group for case-control studies
GEF(Gene-edited female)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
GEF refers to the Myostatin edited female sheep(MSTN-edited female sheep).
Group 0 sample size Number of subjects in the control (unexposed) group
8
Group 1 sample size Number of subjects in the case (exposed) group
8

Lab analysis

Sequencing type
WMS
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
Not specified
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
Mann-Whitney (Wilcoxon)
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
Yes

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged
Chao1 Abundance-based estimator of species richness
unchanged
Simpson Estimator of species richness and species evenness: more weight on species evenness
increased

Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2024-11-27

Curated date: 2024/10/22

Curator: KateRasheed

Revision editor(s): KateRasheed, MyleeeA

Source: Figure 2C, 2E and 2F

Description: Differential abundance of gut microbiota taxa between Gene-edited female (GEF) and Wild-type Female (WTF).

Abundance in Group 1: increased abundance in GEF(Gene-edited female)

NCBI Quality ControlLinks
Agathobaculum
Aminipila
Anaerotignum
Beduinella massiliensis
Clostridiales bacterium
Colidextribacter
Eubacteriaceae bacterium
Evtepia
Evtepia gabavorous
Firmicutes bacterium ADurb.Bin467
Firmicutes bacterium CAG:124
Firmicutes bacterium CAG:170
Flavonifractor
Flavonifractor plautii
Hungatella
Hungatella hathewayi
Intestinimonas
Lactobacillus
Oribacterium
Oscillibacter sp.
Pseudoflavonifractor
Ruthenibacterium
Streptococcus
Subdoligranulum
Bacillota
Synergistota
Chloroflexota
Nitrososphaerota

Revision editor(s): KateRasheed, MyleeeA

Signature 2

Reviewed Marked as Reviewed by Svetlana up on 2024-11-27

Curated date: 2024/10/22

Curator: KateRasheed

Revision editor(s): KateRasheed, MyleeeA

Source: Figure 2C, 2E and 2F

Description: Differential abundance of gut microbiota taxa between Gene-edited female (GEF) and Wild-type Female (WTF).

Abundance in Group 1: decreased abundance in GEF(Gene-edited female)

NCBI Quality ControlLinks
Alloprevotella
Bacteroidia bacterium
Bacteroidota
Phocaeicola vulgatus

Revision editor(s): KateRasheed, MyleeeA

Experiment 2


Reviewed Marked as Reviewed by Svetlana up on 2024-11-27

Curated date: 2024/10/22

Curator: KateRasheed

Revision editor(s): KateRasheed

Differences from previous experiment shown

Subjects

Lab analysis

Statistical Analysis

Statistical test
LEfSe
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
3

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged
Chao1 Abundance-based estimator of species richness
unchanged
Simpson Estimator of species richness and species evenness: more weight on species evenness
increased

Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2024-11-27

Curated date: 2024/10/22

Curator: KateRasheed

Revision editor(s): KateRasheed, MyleeeA

Source: Figure 2G

Description: Differential abundance of gut microbiota taxa between Gene-edited female (GEF) and Wild-type Female (WTF) using LEfSe.

Abundance in Group 1: increased abundance in GEF(Gene-edited female)

NCBI Quality ControlLinks
Bacillota
Clostridia
Clostridiales bacterium
Eubacteriales

Revision editor(s): KateRasheed, MyleeeA

Signature 2

Reviewed Marked as Reviewed by Svetlana up on 2024-11-27

Curated date: 2024/10/22

Curator: KateRasheed

Revision editor(s): KateRasheed, MyleeeA

Source: Figure 2G

Description: Differential abundance of gut microbiota taxa between Gene-edited female (GEF) and Wild-type Female (WTF) using LEfSe.

Abundance in Group 1: decreased abundance in GEF(Gene-edited female)

NCBI Quality ControlLinks
Bacteroidales
Bacteroidia
Bacteroidota
Prevotellaceae

Revision editor(s): KateRasheed, MyleeeA

Experiment 3


Reviewed Marked as Reviewed by Svetlana up on 2024-11-27

Curated date: 2024/10/22

Curator: KateRasheed

Revision editor(s): KateRasheed, MyleeeA

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
WTM(Wild-type male)
Group 1 name Corresponds to the case (exposed) group for case-control studies
GEM(Gene-edited male)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
GEM refers to the Myostatin edited male sheep(MSTN-edited male sheep).

Lab analysis

Statistical Analysis

Statistical test
Mann-Whitney (Wilcoxon)
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
Yes
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
Not specified

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
decreased
Chao1 Abundance-based estimator of species richness
unchanged
Simpson Estimator of species richness and species evenness: more weight on species evenness
increased

Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2024-11-27

Curated date: 2024/10/22

Curator: KateRasheed

Revision editor(s): KateRasheed, MyleeeA

Source: Supplementary Figure 2C, Supplementary Figure 2D and Supplementary Figure 2F

Description: Differential abundance of gut microbiota taxa between Gene-edited Male (GEM) and Wild-type Male (WTM).

Abundance in Group 1: increased abundance in GEM(Gene-edited male)

NCBI Quality ControlLinks
Agathobaculum
Aminipila
Anaerotignum
Bacillota
Beduinella massiliensis
Chloroflexota
Clostridiales bacterium
Colidextribacter
Eubacteriaceae bacterium
Evtepia
Evtepia gabavorous
Firmicutes bacterium ADurb.Bin467
Firmicutes bacterium CAG:124
Firmicutes bacterium CAG:170
Flavonifractor
Flavonifractor plautii
Hungatella
Hungatella hathewayi
Intestinimonas
Lactobacillus
Nitrososphaerota
Oribacterium
Oscillibacter sp.
Pseudoflavonifractor
Ruthenibacterium
Streptococcus
Subdoligranulum
Synergistota

Revision editor(s): KateRasheed, MyleeeA

Signature 2

Reviewed Marked as Reviewed by Svetlana up on 2024-11-27

Curated date: 2024/10/22

Curator: KateRasheed

Revision editor(s): KateRasheed, MyleeeA

Source: Supplementary Figure 2C, Supplementary Figure 2D and Supplementary Figure 2F

Description: Differential abundance of gut microbiota taxa between Gene-edited Male (GEM) and Wild-type Male (WTM).

Abundance in Group 1: decreased abundance in GEM(Gene-edited male)

NCBI Quality ControlLinks
Alloprevotella
Bacteroidia bacterium
Bacteroidota
Phocaeicola vulgatus

Revision editor(s): KateRasheed, MyleeeA

Experiment 4


Reviewed Marked as Reviewed by Svetlana up on 2024-11-27

Curated date: 2024/10/22

Curator: KateRasheed

Revision editor(s): KateRasheed, MyleeeA

Differences from previous experiment shown

Subjects

Lab analysis

Statistical Analysis

Statistical test
LEfSe
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
3

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
decreased
Chao1 Abundance-based estimator of species richness
unchanged
Simpson Estimator of species richness and species evenness: more weight on species evenness
increased

Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2024-11-27

Curated date: 2024/10/22

Curator: KateRasheed

Revision editor(s): KateRasheed, MyleeeA

Source: Supplementary Figure 2G

Description: Differential abundance of gut microbiota taxa between Gene-edited Male (GEM) and Wild-type Male (WTM) using LEfSe.

Abundance in Group 1: increased abundance in GEM(Gene-edited male)

NCBI Quality ControlLinks
Bacillota
Clostridia
Clostridiales bacterium
Eubacteriales

Revision editor(s): KateRasheed, MyleeeA

Signature 2

Reviewed Marked as Reviewed by Svetlana up on 2024-11-27

Curated date: 2024/10/22

Curator: KateRasheed

Revision editor(s): KateRasheed, MyleeeA

Source: Supplementary Figure 2G

Description: Differential abundance of gut microbiota taxa between Gene-edited Male (GEM) and Wild-type Male (WTM) using LEfSe.

Abundance in Group 1: decreased abundance in GEM(Gene-edited male)

NCBI Quality ControlLinks
Bacteroidales
Bacteroidia
Bacteroidota
Prevotellaceae

Revision editor(s): KateRasheed, MyleeeA