Altered gut microbial profile is associated with abnormal metabolism activity of Autism Spectrum Disorder
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Study information
-
Quality control
- Retracted paper
- Contamination issues suspected
- Batch effect issues suspected
- Uncontrolled confounding suspected
- Results are suspect (various reasons)
- Tags applied
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
Dan Z, Mao X, Liu Q, Guo M, Zhuang Y, Liu Z, Chen K, Chen J, Xu R, Tang J, Qin L, Gu B, Liu K, Su C, Zhang F, Xia Y, Hu Z, Liu X
Journal
Gut microbes
Year
2020
Keywords:
16s rRNA, ASD, gut microbiota, metabolism, metagenomics, neurotransmitter
Autism Spectrum Disorder (ASD) is a severe neurodevelopmental disorder. To enhance the understanding of the gut microbiota structure in ASD children at different ages as well as the relationship between gut microbiota and fecal metabolites, we first used the 16S rRNA sequencing to evaluate the gut microbial population in a cohort of 143 children aged 2-13 years old. We found that the α-diversity of ASD group showed no significant change with age, while the TD group showed increased α-diversity with age, which indicates that the compositional development of the gut microbiota in ASD varies at different ages in ways that are not consistent with TD group. Recent studies have shown that chronic constipation is one of the most commonly obvious gastrointestinal (GI) symptoms along with ASD core symptoms. To further investigate the potential interaction effects between ASD and GI symptoms, the 30 C-ASD and their aged-matched TD were picked out to perform metagenomics analysis. We observed that C-ASD group displayed decreased diversity, depletion of species of Sutterella, Prevotella, and Bacteroides as well as dysregulation of associated metabolism activities, which may involve in the pathogenesis of C-ASD. Consistent with metagenomic analysis, liquid chromatography-mass spectrometry (LC/MS) revealed some of the differential metabolites between C-ASD and TD group were involved in the metabolic network of neurotransmitters including serotonin, dopamine, histidine, and GABA. Furthermore, we found these differences in metabolites were associated with altered abundance of specific bacteria. The study suggested possible future modalities for ASD intervention through targeting the specific bacteria associated with neurotransmitter metabolism.
Experiment 1
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Autism spectrum disorder atypical autism,autism spectrum disease,autism spectrum disorder,Autism Spectrum Disorders,autistic spectrum disorder,CHILD DEVELOPMENT DIS PERVASIVE,Child Development Disorders, Pervasive,Development Disorder, Pervasive,Development Disorders, Pervasive,Disorder, Autism Spectrum,Disorder, Pervasive Development,Disorders, Autism Spectrum,Disorders, Pervasive Development,PDD,PERVASIVE CHILD DEVELOPMENT DIS,Pervasive Child Development Disorders,Pervasive Development Disorder,Pervasive Development Disorders,pervasive developmental disorder - not otherwise specified,pervasive developmental disorders,Spectrum Disorder, Autism,Spectrum Disorders, Autism,Autism spectrum disorder
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Typically developing individuals
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Children with Autism Spectrum Disorder (ASD)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Diagnosed according to the Diagnostic and Statistical Manual of Mental Disorders, 5th Edition
- Group 0 sample size Number of subjects in the control (unexposed) group
- 143
- Group 1 sample size Number of subjects in the case (exposed) group
- 143
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- 3 months
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- LEfSe
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 3.5
- Matched on Factors on which subjects have been matched on in a case-control study
- age, sex
Alpha Diversity
- Richness Number of species
- decreased
Signature 1
Source: Figure 1 f-g
Description: Bacterial taxa differentially abundant between TD and ASD
Abundance in Group 1: increased abundance in Children with Autism Spectrum Disorder (ASD)
Revision editor(s): AlishaM
Signature 2
Source: Figure 1 f-g
Description: Bacterial taxa differentially abundant between TD and ASD
Abundance in Group 1: decreased abundance in Children with Autism Spectrum Disorder (ASD)
| NCBI | Quality Control | Links |
|---|---|---|
| Bacteroidia | ||
| Bacteroidales | ||
| Prevotellaceae | ||
| Prevotella_9Prevotella_9 | ||
| Megamonas | ||
| Ruminococcus_2Ruminococcus_2 |
Revision editor(s): AlishaM
Experiment 2
Differences from previous experiment shown
Subjects
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Children with constipated Autism Spectrum Disorder (C-ASD)
- Group 0 sample size Number of subjects in the control (unexposed) group
- 30
- Group 1 sample size Number of subjects in the case (exposed) group
- 30
Lab analysis
Statistical Analysis
- Statistical test
- Mann-Whitney (Wilcoxon)
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- Not specified
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- increased
- Richness Number of species
- increased
Signature 1
Source: Figure 3F, Table S6
Description: Relative abundance of species enriched in TD versus C-ASD
Abundance in Group 1: decreased abundance in Children with constipated Autism Spectrum Disorder (C-ASD)
Revision editor(s): AlishaM
Experiment 3
Differences from previous experiment shown
Subjects
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Children with Autism Spectrum Disorder (ASD)
- Group 0 sample size Number of subjects in the control (unexposed) group
- 143
- Group 1 sample size Number of subjects in the case (exposed) group
- 143
Lab analysis
Statistical Analysis
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
Signature 1
Source: Table S3
Description: Operational taxonomic units with significantly different relative abundance between ASD and TD group.
Abundance in Group 1: increased abundance in Children with Autism Spectrum Disorder (ASD)
Revision editor(s): AlishaM
Signature 2
Source: Table S3
Description: Operational taxonomic units with significantly different relative abundance between ASD and TD group
Abundance in Group 1: decreased abundance in Children with Autism Spectrum Disorder (ASD)
Revision editor(s): AlishaM
Experiment 4
Differences from previous experiment shown
Subjects
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Children with non-constipated Autism Spectrum Disorder (NC-ASD)
- Group 0 sample size Number of subjects in the control (unexposed) group
- 86
- Group 1 sample size Number of subjects in the case (exposed) group
- 86
Lab analysis
Statistical Analysis
Signature 1
Source: Table S4
Description: Operational taxonomic units, phylum and genus with significantly different relative abundance between NC-ASD and TD group
Abundance in Group 1: increased abundance in Children with non-constipated Autism Spectrum Disorder (NC-ASD)
Revision editor(s): AlishaM
Signature 2
Source: Table S4
Description: Operational taxonomic units, phylum and genus with significantly different relative abundance between NC-ASD and TD group
Abundance in Group 1: decreased abundance in Children with non-constipated Autism Spectrum Disorder (NC-ASD)
Revision editor(s): AlishaM
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