Comparative Analysis of the Gut Microbiota of Bat Species with Different Feeding Habits

Study information

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study design

cross-sectional observational, not case-control

Citation

PMID PubMed identifier for scientific articles.

38927243

DOI Digital object identifier for electronic documents.

10.3390/biology13060363

URI

Authors

Mena Canata DA, Benfato MS, Pereira FD, Ramos Pereira MJ, Hackenhaar FS, Mann MB, Frazzon APG, Rampelotto PH

Journal

Biology

Year

2024

Keywords:

diet, frugivore, insectivore, microbiome, nectarivore, vampire bat

Bats are a diverse and ecologically important group of mammals that exhibit remarkable diversity in their feeding habits. These diverse feeding habits are thought to be reflected in the composition and function of their gut microbiota, which plays important roles in nutrient acquisition, immune function, and overall health. Despite the rich biodiversity of bat species in South America, there is a lack of microbiome studies focusing on bats from this region. Such studies could offer major insights into conservation efforts and the preservation of biodiversity in South America. In this work, we aimed to compare the gut microbiota of four bat species with different feeding habits from Southern Brazil, including nectarivorous, frugivorous, insectivorous, and hematophagous bats. Our findings demonstrate that feeding habits can have a significant impact on the diversity and composition of bat gut microbiotas, with each species exhibiting unique metabolic potentials related to their dietary niches. In addition, the identification of potentially pathogenic bacteria suggests that the carriage of microbial pathogens by bats may vary, depending on feeding habits and host-specific factors. These findings provide novel insights into the relationship between bat feeding habits and gut microbiota composition, highlighting the need to promote diverse habitats and food sources to support these ecologically important species.

Experiment 1

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Curated date: 2024/12/16

Curator: YokoC

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Subjects

Location of subjects

Brazil

Host species Species from which microbiome was sampled. Contact us to have more species added.

Glossophaga soricina

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology

Material entity in digestive tract Digestive tract contents,Ingested material entity,Material entity in digestive tract,material entity in digestive tract

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology

Diet Dietary,Diets,Diet,diet

Group 0 name Corresponds to the control (unexposed) group for case-control studies

Sturnira lilium, Molossus molossus, Desmodus rotundus

Group 1 name Corresponds to the case (exposed) group for case-control studies

Glossophaga soricina

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group

A nectarivorous bat.

Group 0 sample size Number of subjects in the control (unexposed) group

26

Group 1 sample size Number of subjects in the case (exposed) group

7

Lab analysis

Sequencing type

16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene

V4

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance

Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).

relative abundances

Statistical test

LEfSe

Significance threshold p-value or FDR threshold used for differential abundance testing (if any)

0.1

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?

Yes

LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool

2.0

Signature 1

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Curated date: 2024/12/18

Curator: YokoC

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Source: Figure 4

Description: LEfSe analysis of bacteria genera from four bat species with different feeding habits.

Abundance in Group 1: increased abundance in Glossophaga soricina

NCBI	Quality Control	Links
Actinomyces
Terrisporobacter
Ureaplasma
unclassified Clostridiaceae
unclassified Enterobacteriaceae
unclassified Neisseriaceae
Clostridium sensu stricto 1Clostridium sensu stricto 1

Revision editor(s): YokoC

Signature 2

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Curated date: 2024/12/18

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Source: Figure 4

Description: LEfSe analysis of bacteria genera from four bat species with different feeding habits.

Abundance in Group 1: decreased abundance in Glossophaga soricina

NCBI	Quality Control	Links
Atopobium
Campylobacter
Candidatus Arthromitus
Edwardsiella
Fusobacterium
Helicobacter
Ligilactobacillus
Peptostreptococcaceae
unclassified Acholeplasmataceae
unclassified Actinomycetota
unclassified Bacilli
unclassified Erysipelotrichales
unclassified Lactobacillales
unclassified Pasteurellaceae

Revision editor(s): YokoC

Experiment 2

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Curated date: 2024/12/18

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Differences from previous experiment shown

Subjects

Host species Species from which microbiome was sampled. Contact us to have more species added.

Sturnira lilium

Group 0 name Corresponds to the control (unexposed) group for case-control studies

Glossophaga soricina, Molossus molossus, Desmodus rotundus

Group 1 name Corresponds to the case (exposed) group for case-control studies

Sturnira lilium

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group

A frugivorous bat.

Group 0 sample size Number of subjects in the control (unexposed) group

23

Group 1 sample size Number of subjects in the case (exposed) group

10

Lab analysis

Statistical Analysis

Signature 1

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Curated date: 2024/12/18

Curator: YokoC

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Source: Figure 4

Description: LEfSe analysis of bacteria genera from four bat species with different feeding habits.

Abundance in Group 1: increased abundance in Sturnira lilium

NCBI	Quality Control	Links
Campylobacter
Helicobacter
unclassified Acholeplasmataceae

Revision editor(s): YokoC

Signature 2

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Curated date: 2024/12/18

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Source: Figure 4

Description: LEfSe analysis of bacteria genera from four bat species with different feeding habits.

Abundance in Group 1: decreased abundance in Sturnira lilium

NCBI	Quality Control	Links
Actinomyces
Atopobium
Candidatus Arthromitus
Edwardsiella
Fusobacterium
Ligilactobacillus
Peptostreptococcaceae
Terrisporobacter
Ureaplasma
unclassified Actinomycetota
unclassified Bacilli
unclassified Clostridiaceae
unclassified Enterobacteriaceae
unclassified Erysipelotrichales
unclassified Lactobacillales
unclassified Neisseriaceae
unclassified Pasteurellaceae
Clostridium sensu stricto 1Clostridium sensu stricto 1

Revision editor(s): YokoC

Experiment 3

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Curated date: 2024/12/18

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Differences from previous experiment shown

Subjects

Host species Species from which microbiome was sampled. Contact us to have more species added.

Molossus molossus

Group 0 name Corresponds to the control (unexposed) group for case-control studies

Glossophaga soricina, Sturnira lilium, Desmodus rotundus

Group 1 name Corresponds to the case (exposed) group for case-control studies

Molossus molossus

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group

An insectivorous bat.

Lab analysis

Statistical Analysis

Signature 1

EditHistoryDelete

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Needs review

Curated date: 2024/12/18

Curator: YokoC

Revision editor(s): YokoC

Source: Figure 4

Description: LEfSe analysis of bacteria genera from four bat species with different feeding habits.

Abundance in Group 1: increased abundance in Molossus molossus

NCBI	Quality Control	Links
Atopobium
Candidatus Arthromitus
Fusobacterium
Ligilactobacillus
unclassified Actinomycetota
unclassified Bacilli
unclassified Erysipelotrichales
unclassified Lactobacillales
unclassified Pasteurellaceae

Revision editor(s): YokoC

Signature 2

EditHistoryDelete

Mark reviewed

Your review change was submitted

Needs review

Curated date: 2024/12/18

Curator: YokoC

Revision editor(s): YokoC

Source: Figure 4

Description: LEfSe analysis of bacteria genera from four bat species with different feeding habits.

Abundance in Group 1: decreased abundance in Molossus molossus

NCBI	Quality Control	Links
Actinomyces
Campylobacter
Edwardsiella
Helicobacter
Peptostreptococcaceae
Terrisporobacter
Ureaplasma
unclassified Acholeplasmataceae
unclassified Clostridiaceae
unclassified Enterobacteriaceae
unclassified Neisseriaceae
Clostridium sensu stricto 1Clostridium sensu stricto 1

Revision editor(s): YokoC

Experiment 4

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Curated date: 2024/12/18

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Differences from previous experiment shown

Subjects

Host species Species from which microbiome was sampled. Contact us to have more species added.

Desmodus rotundus

Group 0 name Corresponds to the control (unexposed) group for case-control studies

Glossophaga soricina, Sturnira lilium, Molossus molossus

Group 1 name Corresponds to the case (exposed) group for case-control studies

Desmodus rotundus

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group

A hematophagous bat.

Group 0 sample size Number of subjects in the control (unexposed) group

27

Group 1 sample size Number of subjects in the case (exposed) group

6

Lab analysis

Statistical Analysis

Signature 1

EditHistoryDelete

Mark reviewed

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Needs review

Curated date: 2024/12/18

Curator: YokoC

Revision editor(s): YokoC

Source: Figure 4

Description: LEfSe analysis of bacteria genera from four bat species with different feeding habits.

Abundance in Group 1: increased abundance in Desmodus rotundus

NCBI	Quality Control	Links
Edwardsiella
Peptostreptococcaceae

Revision editor(s): YokoC

Signature 2

EditHistoryDelete

Mark reviewed

Your review change was submitted

Needs review

Curated date: 2024/12/18

Curator: YokoC

Revision editor(s): YokoC

Source: Figure 4

Description: LEfSe analysis of bacteria genera from four bat species with different feeding habits.

Abundance in Group 1: decreased abundance in Desmodus rotundus

NCBI	Quality Control	Links
Actinomyces
Atopobium
Campylobacter
Candidatus Arthromitus
Fusobacterium
Helicobacter
Ligilactobacillus
Terrisporobacter
Ureaplasma
unclassified Acholeplasmataceae
unclassified Actinomycetota
unclassified Bacilli
unclassified Clostridiaceae
unclassified Enterobacteriaceae
unclassified Erysipelotrichales
unclassified Lactobacillales
unclassified Neisseriaceae
unclassified Pasteurellaceae
Clostridium sensu stricto 1Clostridium sensu stricto 1

Revision editor(s): YokoC