Multiple sclerosis patients have a distinct gut microbiota compared to healthy controls

Study information

Needs review

study design

case-control

Citation

PMID PubMed identifier for scientific articles.

27346372

DOI Digital object identifier for electronic documents.

10.1038/srep28484

URI Uniform resource identifier for web resources.

Authors

Chen J, Chia N, Kalari KR, Yao JZ, Novotna M, Paz Soldan MM, Luckey DH, Marietta EV, Jeraldo PR, Chen X, Weinshenker BG, Rodriguez M, Kantarci OH, Nelson H, Murray JA, Mangalam AK

Journal

Scientific reports

Year

2016

Multiple sclerosis (MS) is an immune-mediated disease, the etiology of which involves both genetic and environmental factors. The exact nature of the environmental factors responsible for predisposition to MS remains elusive; however, it's hypothesized that gastrointestinal microbiota might play an important role in pathogenesis of MS. Therefore, this study was designed to investigate whether gut microbiota are altered in MS by comparing the fecal microbiota in relapsing remitting MS (RRMS) (n = 31) patients to that of age- and gender-matched healthy controls (n = 36). Phylotype profiles of the gut microbial populations were generated using hypervariable tag sequencing of the V3-V5 region of the 16S ribosomal RNA gene. Detailed fecal microbiome analyses revealed that MS patients had distinct microbial community profile compared to healthy controls. We observed an increased abundance of Psuedomonas, Mycoplana, Haemophilus, Blautia, and Dorea genera in MS patients, whereas control group showed increased abundance of Parabacteroides, Adlercreutzia and Prevotella genera. Thus our study is consistent with the hypothesis that MS patients have gut microbial dysbiosis and further study is needed to better understand their role in the etiopathogenesis of MS.

Experiment 1

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Curated date: 2021/01/10

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Subjects

Location of subjects: United States of America

Host species Species from which microbiome was sampled (if applicable): Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: multiple sclerosis Disseminated Sclerosis,generalized multiple sclerosis,insular sclerosis,MS,MS (Multiple Sclerosis),multiple sclerosis,MULTIPLE SCLEROSIS ACUTE FULMINATING,Multiple Sclerosis, Acute Fulminating,Sclerosis, Disseminated,Sclerosis, Multiple

Group 0 name Corresponds to the control (unexposed) group for case-control studies: healthy controls
Group 1 name Corresponds to the case (exposed) group for case-control studies: multiple sclerosis
Group 0 sample size Number of subjects in the control (unexposed) group: 36
Group 1 sample size Number of subjects in the case (exposed) group: 31
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any): current

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V3-V5

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Statistical Analysis

Statistical test: Mann-Whitney (Wilcoxon)

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: Yes

Matched on Factors on which subjects have been matched on in a case-control study: age, sex

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: unchanged

Signature 1

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Curated date: 2019-07-24

Curator: Fatima Zohra

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Source: Figure 3, Table 2

Description: microbial signatures of the gut microbiota of MS patients

Abundance in Group 1: increased abundance in multiple sclerosis

NCBI	Links
Sphingobacteriaceae
Lachnospiraceae
Caulobacteraceae
Flavobacteriaceae
Azotobacter group
Flavobacterium ⚠
Pedobacter ⚠
Blautia
Dorea
Mycoplana
Pseudomonas ⚠

Revision editor(s): WikiWorks743

Signature 2

Edit History Delete

Mark reviewed

Your review change was submitted

Needs review

Curated date: 2019-07-24

Curator: Fatima Zohra

Revision editor(s): WikiWorks743

Source: Figure 3, Table 2

Description: microbial signatures of the gut microbiota of MS patients

Abundance in Group 1: decreased abundance in multiple sclerosis

NCBI	Links
Actinomycetota
Pseudomonadota
Coriobacteriaceae
Porphyromonadaceae
Erysipelotrichaceae
Lactobacillaceae
Veillonellaceae
Enterobacteriaceae
Pasteurellaceae
Collinsella
Parabacteroides
Coprobacillus
Lactobacillus
Haemophilus

Revision editor(s): WikiWorks743