Influence of food consumption patterns and Galician lifestyle on human gut microbiota

From BugSigDB
Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
Castro-Penalonga M, Roca-Saavedra P, Miranda JM, Porto-Arias JJ, Nebot C, Cardelle-Cobas A, Franco CM, Cepeda A
Journal
Journal of physiology and biochemistry
Year
2018
Keywords:
Actinobacteria, Atlantic Diet, Bifidobacterium, Gut microbiota, Lactobacillus, Southern European Atlantic Diet
The proportion of different microbial populations in the human gut is an important factor that in recent years has been linked to obesity and numerous metabolic diseases. Because there are many factors that can affect the composition of human gut microbiota, it is of interest to have information about what is the composition of the gut microbiota in different populations in order to better understand the possibilities for improving nutritional management. A group of 31 volunteers were selected according to established inclusion and exclusion criteria and were asked about their diet history, lifestyle patterns, and adherence to the Southern European Atlantic Diet. Fecal samples were taken and subsequently analyzed by real-time PCR. The results indicated different dietary patterns for subjects who consumed a higher amount of fruits, vegetables, legumes, and fish and a lower amount of bakery foods and precooked foods and snacks compared to Spanish consumption data. Most participants showed intermediate or high adherence to Southern European Atlantic Diet, and an analysis of gut microbiota showed high numbers of total bacteria and Actinobacteria, as well as high amounts of bacteria belonging to the genera Lactobacillus spp. and Bifidobacterium spp. A subsequent statistical comparison also revealed differences in gut microbiota depending on the subject's body weight, age, or degree of adherence to the Southern European Atlantic Diet.

Experiment 1


Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, Victoria

Subjects

Location of subjects
Spain
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Diet Dietary,Diets,Diet,diet
Group 0 name Corresponds to the control (unexposed) group for case-control studies
low Southern European Atlantic Diet
Group 1 name Corresponds to the case (exposed) group for case-control studies
high Southern European Atlantic Diet
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
SEAD: Southern European Atlantic Diet. SEAD adherence was measured using an index of nine food components (fresh fish, cod, red meat and pork products, dairy products, legumes and vegetables, vegetable soup, po- tatoes, wholegrain bread and wine) that ranged from 0 (lowest adherence) to 9 (highest adherence).
Group 0 sample size Number of subjects in the control (unexposed) group
3
Group 1 sample size Number of subjects in the case (exposed) group
7
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
6 months

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
Not specified
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
RT-qPCR

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
ANOVA
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No


Signature 1

Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

Curated date: 2021/01/10

Curator: Lora Kasselman

Revision editor(s): WikiWorks

Source: Table 5

Description: Bacterial groups quantified in human fecal samples in subjects with low, intermediate, and high adherence to Southern European Atlantic Diet (SEAD)

Abundance in Group 1: increased abundance in high Southern European Atlantic Diet

NCBI Quality ControlLinks
Bifidobacterium

Revision editor(s): WikiWorks

Experiment 2


Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, Victoria

Differences from previous experiment shown

Subjects

Group 1 name Corresponds to the case (exposed) group for case-control studies
intermediate Southern European Atlantic Diet
Group 1 sample size Number of subjects in the case (exposed) group
21

Lab analysis

Statistical Analysis

Signature 1

Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

Curated date: 2021/01/10

Curator: Lora Kasselman

Revision editor(s): WikiWorks

Source: Table 5

Description: Bacterial groups quantified in human fecal samples in subjects with low, intermediate, and high adherence to Southern European Atlantic Diet (SEAD)

Abundance in Group 1: increased abundance in intermediate Southern European Atlantic Diet

NCBI Quality ControlLinks
Bifidobacterium

Revision editor(s): WikiWorks

Experiment 3


Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, Victoria

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
<20 years participants
Group 1 name Corresponds to the case (exposed) group for case-control studies
> 30 years participants
Group 0 sample size Number of subjects in the control (unexposed) group
5
Group 1 sample size Number of subjects in the case (exposed) group
11

Lab analysis

Statistical Analysis

Signature 1

Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

Curated date: 2021/01/10

Curator: Lora Kasselman

Revision editor(s): WikiWorks

Source: Table 4

Description: Gut microbiota composition in subjects of different age

Abundance in Group 1: increased abundance in > 30 years participants

NCBI Quality ControlLinks
Pseudomonadota

Revision editor(s): WikiWorks

Experiment 4


Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, Victoria

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
>20 years participants
Group 1 name Corresponds to the case (exposed) group for case-control studies
20-30 years participants
Group 1 sample size Number of subjects in the case (exposed) group
15

Lab analysis

Statistical Analysis

Signature 1

Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

Curated date: 2021/01/10

Curator: Shaimaa Elsafoury

Revision editor(s): WikiWorks

Source: Table 4

Description: Gut microbiota composition in subjects of different age

Abundance in Group 1: increased abundance in 20-30 years participants

NCBI Quality ControlLinks
Pseudomonadota

Revision editor(s): WikiWorks

Experiment 5


Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, Victoria

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
20-30 years participants
Group 1 name Corresponds to the case (exposed) group for case-control studies
> 30 years participants
Group 0 sample size Number of subjects in the control (unexposed) group
15
Group 1 sample size Number of subjects in the case (exposed) group
11

Lab analysis

Statistical Analysis

Signature 1

Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

Curated date: 2021/01/10

Curator: Shaimaa Elsafoury

Revision editor(s): WikiWorks

Source: Table 4

Description: Gut microbiota composition in subjects of different age

Abundance in Group 1: increased abundance in > 30 years participants

NCBI Quality ControlLinks
Lactobacillus

Revision editor(s): WikiWorks

Experiment 6


Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, Victoria

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
BMI < 20
Group 1 name Corresponds to the case (exposed) group for case-control studies
BMI >25
Group 0 sample size Number of subjects in the control (unexposed) group
5

Lab analysis

Statistical Analysis

Signature 1

Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

Curated date: 2021/01/10

Curator: Shaimaa Elsafoury

Revision editor(s): WikiWorks

Source: Table 3

Description: Bacterial groups quantified in human fecal samples in subjects with different body mass index (BMI)

Abundance in Group 1: increased abundance in BMI >25

NCBI Quality ControlLinks
Actinomycetota
Bacteroidota

Revision editor(s): WikiWorks

Signature 2

Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

Curated date: 2021/01/10

Curator: Shaimaa Elsafoury

Revision editor(s): WikiWorks

Source: Table 3

Description: Bacterial groups quantified in human fecal samples in subjects with different body mass index (BMI)

Abundance in Group 1: decreased abundance in BMI >25

NCBI Quality ControlLinks
Bifidobacterium

Revision editor(s): WikiWorks

Experiment 7


Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, Victoria

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
BMI <20
Group 1 name Corresponds to the case (exposed) group for case-control studies
BMI 20-25
Group 1 sample size Number of subjects in the case (exposed) group
15

Lab analysis

Statistical Analysis

Signature 1

Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

Curated date: 2021/01/10

Curator: Shaimaa Elsafoury

Revision editor(s): WikiWorks

Source: Table 3

Description: Bacterial groups quantified in human fecal samples in subjects with different body mass index (BMI)

Abundance in Group 1: decreased abundance in BMI 20-25

NCBI Quality ControlLinks
Bifidobacterium

Revision editor(s): WikiWorks

Experiment 8


Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, Victoria

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
BMI 20-25
Group 1 name Corresponds to the case (exposed) group for case-control studies
BMI >25
Group 0 sample size Number of subjects in the control (unexposed) group
15
Group 1 sample size Number of subjects in the case (exposed) group
11

Lab analysis

Statistical Analysis

Signature 1

Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

Curated date: 2021/01/10

Curator: Shaimaa Elsafoury

Revision editor(s): WikiWorks

Source: Table 3

Description: Bacterial groups quantified in human fecal samples in subjects with different body mass index (BMI)

Abundance in Group 1: increased abundance in BMI >25

NCBI Quality ControlLinks
Actinomycetota

Revision editor(s): WikiWorks