The Microbiome of Prostate Fluid Is Associated With Prostate Cancer

Study information

Reviewed Marked as Reviewed by Peace Sandy on 2024-1-29

study design

cross-sectional observational, not case-control

Citation

PMID PubMed identifier for scientific articles.

31379800

DOI Digital object identifier for electronic documents.

10.3389/fmicb.2019.01664

URI

Authors

Ma X, Chi C, Fan L, Dong B, Shao X, Xie S, Li M, Xue W

Journal

Frontiers in microbiology

Year

2019

Keywords:

cancer, microbiome, prostate, prostate cancer, prostatic fluid

Objectives: To explore the microbiome of the prostatic fluid in high prostate-specific antigen (PSA) patients. Patients and Methods: The microbiome profiles of prostatic fluid samples from 32 prostate cancer (PCa) patients and 27 non-PCa people were assessed. Microbiome analysis was assessed by massive 16S ribosomal RNA gene sequencing. Results: Compared with the NCA group, the microbial diversity was lower in the CA group. There were no specific microbial species in the CA group or NCA group. However, many species, such as those in the genera Alkaliphilus, Enterobacter, Lactococcus, Cronobacter, Carnobacterium, and Streptococcus, showed a significant difference between the CA group and NCA group. Conclusion: The prostate contains reduced bacteria, suggesting a possible pathophysiological correlation between the composition of the microbiome and PCa. Meanwhile, this study uncovered that the microbiome may be beneficial in maintaining the stability of the microenvironment of the prostate and provides interesting perspectives for the identification of novel biomarkers in high-PSA patients.

Experiment 1

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Reviewed Marked as Reviewed by Peace Sandy on 2024-1-29

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): Fatima, WikiWorks, Peace Sandy

Subjects

Location of subjects: China

Host species Species from which microbiome was sampled. Contact us to have more species added.: Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Prostate gland secretion Prostate fluid,Prostate secretion,Prostatic fluid,Secretion of prostate,Secretion of prostate gland,Prostate gland secretion,prostate gland secretion

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Prostate cancer cancer of prostate gland,hereditary prostate cancer,malignant neoplasm of prostate,malignant neoplasm of prostate gland,malignant neoplasm of the prostate,malignant prostate gland neoplasm,malignant prostate neoplasm,malignant prostate tumor,malignant tumor of prostate,malignant tumor of the prostate,NGP - new growth of prostate,prostate cancer,prostate cancer, familial,prostate gland cancer,prostate neoplasm,prostatic cancer,prostatic neoplasm,tumor of the prostate,Prostate cancer

Group 0 name Corresponds to the control (unexposed) group for case-control studies: non-prostate cancer

Group 1 name Corresponds to the case (exposed) group for case-control studies: prostate cancer

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: Prostate cancer commonest male malignant tumors determined by pathology of prostate biopsy

Group 0 sample size Number of subjects in the control (unexposed) group: 27

Group 1 sample size Number of subjects in the case (exposed) group: 32

Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any): 4 weeks

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V3-V4

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).: relative abundances

Statistical test: Mann-Whitney (Wilcoxon)

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: Yes

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: decreased

Simpson Estimator of species richness and species evenness: more weight on species evenness: increased

Richness Number of species: unchanged

Signature 1

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Reviewed Marked as Reviewed by Peace Sandy on 2024-1-29

Curated date: 2021/01/10

Curator: Rimsha Azhar

Revision editor(s): WikiWorks, Peace Sandy

Source: Figure 5

Description: Analysis of the specific microbial species differences. (A) The differences in microbial species between the two groups were explored.

Abundance in Group 1: increased abundance in prostate cancer

NCBI	Quality Control	Links
Carnobacterium
Geobacillus
Lactococcus
Streptococcus

Revision editor(s): WikiWorks, Peace Sandy

Signature 2

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Reviewed Marked as Reviewed by Peace Sandy on 2024-1-29

Curated date: 2021/01/10

Curator: Rimsha Azhar

Revision editor(s): WikiWorks, Peace Sandy

Source: Figure 5

Description: Analysis of the specific microbial species differences. (A) The differences in microbial species between the two groups were explored.

Abundance in Group 1: decreased abundance in prostate cancer

NCBI	Quality Control	Links
Alkaliphilus
Cronobacter
Enterococcus
Paenibacillus

Revision editor(s): WikiWorks, Peace Sandy