Relationship between acetaldehyde concentration in mouth air and characteristics of microbiota of tongue dorsum in Japanese healthy adults: a cross-sectional study

Study information

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study design

cross-sectional observational, not case-control

Citation

PMID PubMed identifier for scientific articles.

31215600

DOI Digital object identifier for electronic documents.

10.1590/1678-7757-2018-0635

URI

Authors

Yokoi A, Ekuni D, Hata H, Yamane-Takeuchi M, Maruyama T, Yamanaka R, Morita M

Journal

Journal of applied oral science : revista FOB

Year

2019

OBJECTIVE: Acetaldehyde, associated with consumption of alcoholic beverages, is known to be a carcinogen and to be related to the tongue dorsum. The aim of this study was to investigate the relationship between acetaldehyde concentration in mouth air and bacterial characteristics on the tongue dorsum. METHODOLOGY: Thirty-nine healthy volunteers participated in the study. Acetaldehyde concentrations in mouth air were evaluated by a high-sensitivity semiconductor gas sensor. A 16S rRNA gene sequencing technique was used to compare microbiomes between two groups, focusing on the six samples with the highest acetaldehyde concentrations (HG) and the six samples with lowest acetaldehyde concentrations (LG). RESULTS: Acetaldehyde concentration increased in correlation with the increase in bacterial count (p=0.048). The number of species observed in the oral microbiome of the HG was higher than that in the oral microbiome of the LG (p=0.011). The relative abundances of Gemella sanguinis, Veillonella parvula and Neisseria flavescens in the oral microbiome of the HG were higher than those in the oral microbiome of the LG (p<0.05). CONCLUSION: Acetaldehyde concentration in mouth air was associated with bacterial count, diversity of microbiome, and relative abundance of G. sanguinis, V. parvula, and N. flavescens.

Experiment 1

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Curated date: 2021/01/10

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Subjects

Location of subjects: Japan

Host species Species from which microbiome was sampled. Contact us to have more species added.: Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Mouth Adult mouth,Cavital oralis,Cavitas oris,Cavum oris,Mouth cavity,Oral region,Oral vestibule,Regio oralis,Rima oris,Stoma,Stomatodaeum,Trophic apparatus,Vestibule of mouth,Vestibulum oris,Mouth,mouth

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Acetaldehyde Acetaldehyd,ACETALDEHYDE,Acetaldehyde,acetaldehyde,acetaldehydes,acetic aldehyde,Azetaldehyd,C2H4O,Ethanal,ethyl aldehyde

Group 0 name Corresponds to the control (unexposed) group for case-control studies: low acetaldehyde concentration group

Group 1 name Corresponds to the case (exposed) group for case-control studies: high acetaldehyde concentration group

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: The Sensor Gas Chromatograph SGEA-P2 (FIS Inc., Itami, Japan) was used to measure acetaldehyde concentrations in mouth air.

Group 0 sample size Number of subjects in the control (unexposed) group: 6

Group 1 sample size Number of subjects in the case (exposed) group: 6

Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any): current use

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V3-V4

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Statistical Analysis

Statistical test: Mann-Whitney (Wilcoxon)

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: No

Signature 1

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Curated date: 2021/01/10

Curator: Shaimaa Elsafoury

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Source: Figure 4

Description: Difference in relative abundance of tongue microbiome between High acetaldehyde concentration group and Low acetaldehyde concentration group.

Abundance in Group 1: increased abundance in high acetaldehyde concentration group

NCBI	Quality Control	Links
Gemella sanguinis
Veillonella parvula
Neisseria flavescens

Revision editor(s): WikiWorks

Signature 2

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Curated date: 2021/01/10

Curator: Shaimaa Elsafoury

Revision editor(s): WikiWorks

Source: Figure 4

Description: Difference in relative abundance of tongue microbiome between High acetaldehyde concentration group and Low acetaldehyde concentration group.

Abundance in Group 1: decreased abundance in high acetaldehyde concentration group

NCBI	Quality Control	Links
Streptococcus parasanguinis
Prevotella histicola

Revision editor(s): WikiWorks