Oral microbiota dysbiosis and its association with Henoch-Schönlein Purpura in children

Study information

Needs review

study design

case-control

Citation

PMID PubMed identifier for scientific articles.

30342346

DOI Digital object identifier for electronic documents.

10.1016/j.intimp.2018.10.017

URI

Authors

Chen B, Wang J, Wang Y, Zhang J, Zhao C, Shen N, Yang J, Gai Z, Zhang L

Journal

International immunopharmacology

Year

2018

Keywords:

Dysbiosis, Henoch-Schönlein Purpura, Immunoglobulin, Oral microbiota

BACKGROUND: The pathogenesis of microbes in allergic diseases has been demonstrated and our previous research indicates that microbiota causing gut disorders in children is associated with Henoch-Schönlein Purpura. However, the role of oral microbiota in Henoch-Schönlein Purpura remains unknown. METHOD: A total of 164 children were enrolled, of which 98 were patients with HSP and 66 were healthy children. Oral swab samples were collected for DNA extraction and 16S rRNA gene sequencing, then analyzed for oral microbiota composition. RESULTS: Oral microbiota differed between healthy children and those with HSP. Children with HSP exhibited higher oral microbial diversity and richness than the controls. Firmicutes, Proteobacteria, and Bacteroidetes are the dominant phyla in children with HSP. We used linear discriminant analysis (LDA) effect size (LEfSe) algorithm and detected 21 bacterial taxonomic clades showing statistical differences (12 increased and 9 decreased) in children with HSP. The correlation analyses between clinical data and abundance in microbial community indicated that an abundance of Butyrivibrio sp. negatively correlated with the length of hospital stay (LOS). Haemophilus sp. negatively correlated to IgE and IgM but positively correlated to LOS, with decreasing significantly in patients with HSP. Prevotella positively correlated with IgM. Prevotella nanceiensis positively correlated with IgA, and were abundant in children with HSP. CONCLUSIONS: These results indicate that children with HSP have significantly different oral microbiota compared to healthy children. Although this study does not imply causality, it is helpful to identify the types and pathways of bacteria that can be used to prevent or treat HSP.

Experiment 1

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Curated date: 2021/01/10

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Subjects

Location of subjects: China

Host species Species from which microbiome was sampled. Contact us to have more species added.: Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Mouth Adult mouth,Cavital oralis,Cavitas oris,Cavum oris,Mouth cavity,Oral region,Oral vestibule,Regio oralis,Rima oris,Stoma,Stomatodaeum,Trophic apparatus,Vestibule of mouth,Vestibulum oris,Mouth,mouth

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Henoch-Schoenlein purpura Allergic purpura,allergic purpura,Allergic purpura (disorder),Allergic purpura NOS (disorder),autoimmune purpura,Autoimmune purpura (disorder) [Ambiguous],Henoch-Schoenlein purpura,Henoch-Scholein purpura,Henoch-Schonlein Purpura,Henoch-Schonlein purpura,Henoch-Schonlein purpura (disorder),Henoch-Schönlein purpura,HSP,IgA vasculitis,Purpura, autoimmune,purpura, autoimmune,Purpura, Schoenlein-Henoch,Purpura: [allergic] or [Henoch-Schonlein allergy],Schoenlein-Henoch purpura,henoch-Schoenlein purpura

Group 0 name Corresponds to the control (unexposed) group for case-control studies: controls

Group 1 name Corresponds to the case (exposed) group for case-control studies: Henoch-Schönlein Purpura

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: Henoch-Schönlein Purpura in children

Group 0 sample size Number of subjects in the control (unexposed) group: 66

Group 1 sample size Number of subjects in the case (exposed) group: 98

Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any): 4 weeks

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V1-V2

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).: relative abundances

Statistical test: LEfSe

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: No

LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool: 3.5

Matched on Factors on which subjects have been matched on in a case-control study: age, sex

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: increased

Richness Number of species: increased

Signature 1

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Curated date: 2021/01/10

Curator: Rimsha Azhar

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Source: Figure 6b, 6c

Description: Histogram of the LDA scores computed for different abundance levels between children with HSP and healthy children, as detected by the LEfSe tool

Abundance in Group 1: increased abundance in Henoch-Schönlein Purpura

NCBI	Quality Control	Links
Neisseriales
Neisseriaceae
Neisseria
Veillonella
Negativicutes
Veillonellales
Veillonellaceae
Prevotella
Prevotellaceae
Bacteroidota
Bacteroidia
Bacteroidales

Revision editor(s): WikiWorks

Signature 2

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Needs review

Curated date: 2021/01/10

Curator: Rimsha Azhar

Revision editor(s): WikiWorks

Source: Figure 6b, 6c

Description: Histogram of the LDA scores computed for different abundance levels between children with HSP and healthy children, as detected by the LEfSe tool

Abundance in Group 1: decreased abundance in Henoch-Schönlein Purpura

NCBI	Quality Control	Links
Haemophilus
Pasteurellales
Pasteurellaceae
Alphaproteobacteria
Acinetobacter
Moraxellaceae
Pseudomonadales
Gammaproteobacteria
Pseudomonadota

Revision editor(s): WikiWorks