Oral microbiota dysbiosis and its association with Henoch-Schönlein Purpura in children/Experiment 1
From BugSigDB
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Mouth Adult mouth,Cavital oralis,Cavitas oris,Cavum oris,Mouth cavity,Oral region,Oral vestibule,Regio oralis,Rima oris,Stoma,Stomatodaeum,Trophic apparatus,Vestibule of mouth,Vestibulum oris,Mouth,mouth
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Henoch-Schoenlein purpura Allergic purpura,allergic purpura,Allergic purpura (disorder),Allergic purpura NOS (disorder),autoimmune purpura,Autoimmune purpura (disorder) [Ambiguous],Henoch-Schoenlein purpura,Henoch-Scholein purpura,Henoch-Schonlein Purpura,Henoch-Schonlein purpura,Henoch-Schonlein purpura (disorder),Henoch-Schönlein purpura,HSP,IgA vasculitis,Purpura, autoimmune,purpura, autoimmune,Purpura, Schoenlein-Henoch,Purpura: [allergic] or [Henoch-Schonlein allergy],Schoenlein-Henoch purpura,henoch-Schoenlein purpura
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- controls
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Henoch-Schönlein Purpura
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Henoch-Schönlein Purpura in children
- Group 0 sample size Number of subjects in the control (unexposed) group
- 66
- Group 1 sample size Number of subjects in the case (exposed) group
- 98
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- 4 weeks
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V1-V2
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- LEfSe
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 3.5
- Matched on Factors on which subjects have been matched on in a case-control study
- age, sex
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- increased
- Richness Number of species
- increased
Signature 1
Needs review
Source: Figure 6b, 6c
Description: Histogram of the LDA scores computed for different abundance levels between children with HSP and healthy children, as detected by the LEfSe tool
Abundance in Group 1: increased abundance in Henoch-Schönlein Purpura
| NCBI | Quality Control | Links |
|---|---|---|
| Neisseriales | ||
| Neisseriaceae | ||
| Neisseria | ||
| Veillonella | ||
| Negativicutes | ||
| Veillonellales | ||
| Veillonellaceae | ||
| Prevotella | ||
| Prevotellaceae | ||
| Bacteroidota | ||
| Bacteroidia | ||
| Bacteroidales |
Revision editor(s): WikiWorks
Signature 2
Needs review
Source: Figure 6b, 6c
Description: Histogram of the LDA scores computed for different abundance levels between children with HSP and healthy children, as detected by the LEfSe tool
Abundance in Group 1: decreased abundance in Henoch-Schönlein Purpura
| NCBI | Quality Control | Links |
|---|---|---|
| Haemophilus | ||
| Pasteurellales | ||
| Pasteurellaceae | ||
| Alphaproteobacteria | ||
| Acinetobacter | ||
| Moraxellaceae | ||
| Pseudomonadales | ||
| Gammaproteobacteria | ||
| Pseudomonadota |
Revision editor(s): WikiWorks
