Diverse vaginal microbiomes in reproductive-age women with vulvovaginal candidiasis

From BugSigDB
Reviewed Marked as Reviewed by Claregrieve1 on 2022/08/31
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
Liu MB, Xu SR, He Y, Deng GH, Sheng HF, Huang XM, Ouyang CY, Zhou HW
Journal
PloS one
Year
2013
Vulvovaginal candidiasis (VVC) is one of the most prevalent vaginal infectious diseases, and there are controversial reports regarding the diversity of the associated vaginal microbiota. We determined the vaginal microbial community in patients with VVC, bacterial vaginosis (BV), and mixed infection of VVC and BV using Illumina sequencing of 16S rRNA tags. Our results revealed for the first time the highly variable patterns of the vaginal microbiome from VVC patients. In general, the alpha-diversity results of species richness and evenness showed the following order: normal control < VVC only < mixed BV and VVC infection < BV only. The beta-diversity comparison of community structures also showed an intermediate composition of VVC between the control and BV samples. A detailed comparison showed that, although the control and BV communities had typical patterns, the vaginal microbiota of VVC is complex. The mixed BV and VVC infection group showed a unique pattern, with a relatively higher abundance of Lactobacillus than the BV group and higher abundance of Prevotella, Gardnerella, and Atopobium than the normal control. In contrast, the VVC-only group could not be described by any single profile, ranging from a community structure similar to the normal control (predominated with Lactobacillus) to BV-like community structures (abundant with Gardnerella and Atopobium). Treatment of VVC resulted in inconsistent changes of the vaginal microbiota, with four BV/VVC samples recovering to a higher Lactobacillus level, whereas many VVC-only patients did not. These results will be useful for future studies on the role of vaginal microbiota in VVC and related infectious diseases.

Experiment 1


Reviewed Marked as Reviewed by Claregrieve1 on 2022/08/31

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): Claregrieve1, WikiWorks, Victoria

Subjects

Location of subjects
China
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Vagina Vagina,vagina,Distal oviductal region,Distal portion of oviduct,Vaginae
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Vulvovaginitis Vulvo-vaginitis,Vulvovaginitis,vulvovaginitis
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Normal controls
Group 1 name Corresponds to the case (exposed) group for case-control studies
Women with BV
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
BV assessment with a gram-stain score => 7
Group 0 sample size Number of subjects in the control (unexposed) group
30
Group 1 sample size Number of subjects in the case (exposed) group
10
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
1 month

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V6
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
LEfSe
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
increased

Signature 1

Reviewed Marked as Reviewed by Claregrieve1 on 2022/08/31

Curated date: 2021/01/10

Curator: Fatima Zohra

Revision editor(s): Claregrieve1, WikiWorks

Source: Supplemental Figure 1

Description: Differential microbial abundance between normal controls and women with BV

Abundance in Group 1: increased abundance in Women with BV

NCBI Quality ControlLinks
Actinomycetota
Actinomycetaceae
Actinomycetales
Atopobium
Bifidobacteriaceae
Bifidobacteriales
Bulleidia
Clostridia
Coriobacteriaceae
Coriobacteriales
Dialister
Erysipelotrichaceae
Erysipelotrichales
Erysipelotrichia
Eubacteriales
Fusobacteriota
Fusobacteriales
Gardnerella
Mobiluncus
Porphyromonadaceae
Porphyromonas
Pseudomonadota
Sneathia
Veillonellaceae
Leptotrichiaceae

Revision editor(s): Claregrieve1, WikiWorks

Signature 2

Reviewed Marked as Reviewed by Claregrieve1 on 2022/08/31

Curated date: 2021/01/10

Curator: Fatima Zohra

Revision editor(s): Claregrieve1, WikiWorks

Source: Supplemental Figure 1

Description: Differential microbial abundance between normal controls and women with BV

Abundance in Group 1: decreased abundance in Women with BV

NCBI Quality ControlLinks
Bacillota
Lactobacillaceae
Lactobacillus

Revision editor(s): Claregrieve1, WikiWorks

Experiment 2


Reviewed Marked as Reviewed by Claregrieve1 on 2022/08/31

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): Claregrieve1, WikiWorks, Victoria

Differences from previous experiment shown

Subjects

Group 1 name Corresponds to the case (exposed) group for case-control studies
Women with BV and VVC
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Women infected with both BV and VVC
Group 1 sample size Number of subjects in the case (exposed) group
16

Lab analysis

Statistical Analysis

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
increased

Signature 1

Reviewed Marked as Reviewed by Claregrieve1 on 2022/08/31

Curated date: 2021/01/10

Curator: Fatima Zohra

Revision editor(s): Claregrieve1, WikiWorks

Source: Supplemental Figure 1

Description: Differential microbial abundance between normal controls and women with candida vulvovaginitis and BV

Abundance in Group 1: increased abundance in Women with BV and VVC

NCBI Quality ControlLinks
Bacteroidales
Bacteroidota
Bacteroidia
Prevotella
Prevotellaceae

Revision editor(s): Claregrieve1, WikiWorks