Diverse vaginal microbiomes in reproductive-age women with vulvovaginal candidiasis
-
Quality control
- Retracted paper
- Contamination issues suspected
- Batch effect issues suspected
- Uncontrolled confounding suspected
- Results are suspect (various reasons)
- Tags applied
Experiment 1
Curated date: 2021/01/10
Curator: WikiWorks743
Revision editor(s): WikiWorks753, WikiWorks743, Claregrieve1
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled (if applicable)
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Vagina Distal oviductal region,Distal portion of oviduct,Vaginae,Vagina
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- vulvovaginitis Vulvo-vaginitis,Vulvovaginitis,vulvovaginitis
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Normal controls
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Women with BV
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- BV assessment with a gram-stain score => 7
- Group 0 sample size Number of subjects in the control (unexposed) group
- 30
- Group 1 sample size Number of subjects in the case (exposed) group
- 10
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- 1 month
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V6
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Statistical test
- LEfSe
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- increased
Signature 1
Source: Supplemental Figure 1
Description: Differential microbial abundance between normal controls and women with BV
Abundance in Group 1: increased abundance in Women with BV
Revision editor(s): WikiWorks743, Claregrieve1
Signature 2
Source: Supplemental Figure 1
Description: Differential microbial abundance between normal controls and women with BV
Abundance in Group 1: decreased abundance in Women with BV
NCBI | Links |
---|---|
Bacillota | |
Lactobacillaceae | |
Lactobacillus |
Revision editor(s): WikiWorks743, Claregrieve1
Experiment 2
Curated date: 2021/01/10
Curator: WikiWorks743
Revision editor(s): WikiWorks753, WikiWorks743, Claregrieve1
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled (if applicable)
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Vagina Distal oviductal region,Distal portion of oviduct,Vaginae,Vagina
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- vulvovaginitis Vulvo-vaginitis,Vulvovaginitis,vulvovaginitis
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Normal controls
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Women with BV and VVC
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Women infected with both BV and VVC
- Group 0 sample size Number of subjects in the control (unexposed) group
- 30
- Group 1 sample size Number of subjects in the case (exposed) group
- 16
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- 1 month
Lab analysis
- Sequencing type
- 16S
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Statistical test
- LEfSe
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- increased
Signature 1
Source: Supplemental Figure 1
Description: Differential microbial abundance between normal controls and women with candida vulvovaginitis and BV
Abundance in Group 1: increased abundance in Women with BV and VVC
NCBI | Links |
---|---|
Bacteroidales | |
Bacteroidota | |
Bacteroidia | |
Prevotella | |
Prevotellaceae |
Revision editor(s): WikiWorks743, Claregrieve1