Gut Microbiota Signatures in Gestational Anemia

Study information

Reviewed Marked as Reviewed by Chinelsy on 2023-10-30

study design

case-control

Citation

PMID PubMed identifier for scientific articles.

33718259

DOI Digital object identifier for electronic documents.

10.3389/fcimb.2021.549678

URI

Authors

Long Y, Liang F, Guo R, Zhu C, Zhao X, Wang X, Liu F, Jiang M, Liang Q, Zeng S, Han M, Qin J, Li S, Li S, Yang H

Journal

Frontiers in cellular and infection microbiology

Year

2021

Keywords:

16S rRNA gene sequencing, gestational anemia, gut microbiota, microbial dysbiosis, pregnant women

Gestational diseases are associated with altered intestinal microbiota in pregnant women. Characterizing the gut microbiota of gestational anemia (GA) may describe a novel role of gut microbial abnormality in GA. In this study, we investigated differences in gut microbiota between GA patients and healthy pregnant women from the first trimester (n = 24 vs. 54) and the third trimester (n = 30 vs. 56) based on the 16S rRNA gene sequencing method. No statistically significant differences in α-diversity were identified between GA patients and controls in the first trimester of pregnancy, whereas the Shannon index and observed OTUs were significantly lower in GA patients than in healthy controls in the third trimester. Distance-based redundancy analysis revealed striking differences in microbial communities in the third trimester between GA patients and controls. Four genera were significantly different in relative abundance between GA patients and healthy controls, while 12 genera differentiated significantly between GA patients and healthy controls in the third trimester. At the operational taxonomic unit (OTU) level, 17 OTUs and 30 OTUs were identified to be different between GA patients and healthy controls in the first and third trimesters, respectively. Changes in gut microbial composition of GA patients suggest a potential relation with GA, and provide insights into the prediction and intervention of gestational anemia.

Experiment 1

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Reviewed Marked as Reviewed by Chinelsy on 2023-10-30

Curated date: 2023/10/29

Curator: Chinelsy

Revision editor(s): Chinelsy, ChiomaBlessing

Subjects

Location of subjects: China

Host species Species from which microbiome was sampled. Contact us to have more species added.: Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Anemia anaemia,anemia,anemia (disease),Anemia

Group 0 name Corresponds to the control (unexposed) group for case-control studies: healthy controls(HC1 group)

Group 1 name Corresponds to the case (exposed) group for case-control studies: gestational anemia (GA1 group)

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: Gestational diseases are associated with altered intestinal microbiota in pregnant women in the first trimester.

Group 0 sample size Number of subjects in the control (unexposed) group: 54

Group 1 sample size Number of subjects in the case (exposed) group: 24

Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any): 4 weeks

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V4

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).: relative abundances

Statistical test: Mann-Whitney (Wilcoxon)

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05

Matched on Factors on which subjects have been matched on in a case-control study: age, body mass index, gestational age, sex

Alpha Diversity

Pielou Quantifies how equal the community is numerically: unchanged

Shannon Estimator of species richness and species evenness: more weight on species richness: unchanged

Richness Number of species: unchanged

Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample: unchanged

Signature 1

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Reviewed Marked as Reviewed by Chinelsy on 2023-10-30

Curated date: 2021/01/10

Curator: Lora Kasselman

Revision editor(s): WikiWorks, Chinelsy, ChiomaBlessing

Source: FIGURE 2B.

Description: Bacterial genera significantly enriched in the GA1 group compared to the HC1 group (first trimester).

Abundance in Group 1: increased abundance in gestational anemia (GA1 group)

NCBI	Quality Control	Links
Anaerotruncus
Oribacterium
Parvimonas
Turicibacter

Revision editor(s): WikiWorks, Chinelsy, ChiomaBlessing

Experiment 2

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Curated date: 2023/10/29

Curator: Chinelsy

Revision editor(s): Chinelsy, ChiomaBlessing

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies: healthy controls (HC3 group)

Group 1 name Corresponds to the case (exposed) group for case-control studies: gestational anemia (GA3 group)

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: Gestational diseases are associated with altered intestinal microbiota in pregnant women in the third trimester.