The influence of in vitro pectin fermentation on the human fecal microbiome/Experiment 1

From BugSigDB


Reviewed Marked as Reviewed by Folakunmi on 2024-1-19

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, Folakunmi

Subjects

Location of subjects
South Korea
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Diet Dietary,Diets,Diet,diet
Group 0 name Corresponds to the control (unexposed) group for case-control studies
late pectin incubation time
Group 1 name Corresponds to the case (exposed) group for case-control studies
early pectin incubation time
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
before/early pectin incubation
Group 0 sample size Number of subjects in the control (unexposed) group
3
Group 1 sample size Number of subjects in the case (exposed) group
3
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
6 months

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V1-V2
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Ion Torrent

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
Linear Regression
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.1
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
Yes


Signature 1

Reviewed Marked as Reviewed by Folakunmi on 2024-1-19

Curated date: 2021/01/10

Curator: Lora Kasselman

Revision editor(s): WikiWorks

Source: Figure 4 and text

Description: Significantly changed taxa according to pectin incubation time (q value < 0.1)

Abundance in Group 1: increased abundance in early pectin incubation time

NCBI Quality ControlLinks
Bifidobacterium
Coriobacteriaceae
Coprobacillus
Peptostreptococcaceae
Oxalobacter
Christensenellaceae
Clostridiaceae
Odoribacter
Coprococcus
Butyricimonas
Ruminococcus
Anaerostipes
Blautia
Parabacteroides
Phascolarctobacterium
Roseburia
Rikenellaceae
Bacteroides

Revision editor(s): WikiWorks

Signature 2

Reviewed Marked as Reviewed by Folakunmi on 2024-1-19

Curated date: 2021/01/10

Curator: Lora Kasselman

Revision editor(s): WikiWorks

Source: Figure 4 and text

Description: Significantly changed taxa according to pectin incubation time (q value < 0.1)

Abundance in Group 1: decreased abundance in early pectin incubation time

NCBI Quality ControlLinks
Lachnospira
Sutterella
Dorea
Clostridium
Burkholderiales
Bacteroidales

Revision editor(s): WikiWorks